Identification and analysis of expressed sequence tags present in xylem tissues of kelampayan (Neolamarckia cadamba (Roxb.) Bosser)
The large-scale genomic resource for kelampayan was generated from a developing xylem cDNA library. A total of 6,622 high quality expressed sequence tags (ESTs) were generated through high-throughput 5’ EST sequencing of cDNA clones. The ESTs were analyzed and assembled to generate 4,728 xylogenesi...
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| Format: | Article |
| Language: | English |
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Springer India
2014
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| Online Access: | http://ir.unimas.my/id/eprint/7134/ http://ir.unimas.my/id/eprint/7134/1/Pang.pdf |
| _version_ | 1848836063420743680 |
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| author | Wei-Seng, Ho Shek-Ling, Pang Julaihi, Abdullah |
| author_facet | Wei-Seng, Ho Shek-Ling, Pang Julaihi, Abdullah |
| author_sort | Wei-Seng, Ho |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | The large-scale genomic resource for kelampayan
was generated from a developing xylem cDNA library. A total of 6,622 high quality expressed sequence tags (ESTs) were generated through high-throughput 5’ EST sequencing of cDNA clones. The ESTs were analyzed and assembled to generate 4,728 xylogenesis unigenes distributed in 2,100 contigs and 2,628 singletons. About 59.3 % of the ESTs were assigned with putative identifications whereas 40.7 % of the sequences showed no significant similarity to any sequences in GenBank. Interestingly, most genes involved in lignin
biosynthesis and several other cell wall biosynthesis genes were identified in the kelampayan EST database. The identified genes in this study will be candidates for functional genomics and association genetic studies in kelampayan aiming at the production of high value forests. |
| first_indexed | 2025-11-15T06:17:48Z |
| format | Article |
| id | unimas-7134 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:17:48Z |
| publishDate | 2014 |
| publisher | Springer India |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-71342021-06-05T05:19:27Z http://ir.unimas.my/id/eprint/7134/ Identification and analysis of expressed sequence tags present in xylem tissues of kelampayan (Neolamarckia cadamba (Roxb.) Bosser) Wei-Seng, Ho Shek-Ling, Pang Julaihi, Abdullah QC Physics QP Physiology SB Plant culture The large-scale genomic resource for kelampayan was generated from a developing xylem cDNA library. A total of 6,622 high quality expressed sequence tags (ESTs) were generated through high-throughput 5’ EST sequencing of cDNA clones. The ESTs were analyzed and assembled to generate 4,728 xylogenesis unigenes distributed in 2,100 contigs and 2,628 singletons. About 59.3 % of the ESTs were assigned with putative identifications whereas 40.7 % of the sequences showed no significant similarity to any sequences in GenBank. Interestingly, most genes involved in lignin biosynthesis and several other cell wall biosynthesis genes were identified in the kelampayan EST database. The identified genes in this study will be candidates for functional genomics and association genetic studies in kelampayan aiming at the production of high value forests. Springer India 2014 Article PeerReviewed text en http://ir.unimas.my/id/eprint/7134/1/Pang.pdf Wei-Seng, Ho and Shek-Ling, Pang and Julaihi, Abdullah (2014) Identification and analysis of expressed sequence tags present in xylem tissues of kelampayan (Neolamarckia cadamba (Roxb.) Bosser). Physiology and Molecular Biology of Plants, 20 (3). pp. 393-397. ISSN 0974-0430 http://www.ncbi.nlm.nih.gov/pubmed/25049467 DOI 10.1007/s12298-014-0230-x |
| spellingShingle | QC Physics QP Physiology SB Plant culture Wei-Seng, Ho Shek-Ling, Pang Julaihi, Abdullah Identification and analysis of expressed sequence tags present in xylem tissues of kelampayan (Neolamarckia cadamba (Roxb.) Bosser) |
| title | Identification and analysis of expressed sequence tags present in xylem tissues of kelampayan (Neolamarckia cadamba (Roxb.) Bosser) |
| title_full | Identification and analysis of expressed sequence tags present in xylem tissues of kelampayan (Neolamarckia cadamba (Roxb.) Bosser) |
| title_fullStr | Identification and analysis of expressed sequence tags present in xylem tissues of kelampayan (Neolamarckia cadamba (Roxb.) Bosser) |
| title_full_unstemmed | Identification and analysis of expressed sequence tags present in xylem tissues of kelampayan (Neolamarckia cadamba (Roxb.) Bosser) |
| title_short | Identification and analysis of expressed sequence tags present in xylem tissues of kelampayan (Neolamarckia cadamba (Roxb.) Bosser) |
| title_sort | identification and analysis of expressed sequence tags present in xylem tissues of kelampayan (neolamarckia cadamba (roxb.) bosser) |
| topic | QC Physics QP Physiology SB Plant culture |
| url | http://ir.unimas.my/id/eprint/7134/ http://ir.unimas.my/id/eprint/7134/ http://ir.unimas.my/id/eprint/7134/ http://ir.unimas.my/id/eprint/7134/1/Pang.pdf |