Polymorphism in the tumor necrosis factor alpha promoter region and its influence on colorectal cancer predisposition risk in Malaysian population
objective:A case control study was designed to investigate the TNF-a -308 G>A polymorphism allele frequencies and to determine the influence of the polymorphic genotype on sporadic CRC susceptibility risk in Malaysia population. Material and methods: Peripheral blood samples of 164 normal contro...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Japan International Cultural Exchange Foundation (JICEF),
2011
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/706/ http://ir.unimas.my/id/eprint/706/1/Polymorphism%20in%20the%20tumor%20necrosis%20factor%20alpha%20promoter.pdf |
| _version_ | 1848834599985086464 |
|---|---|
| author | Mohd Aminudin, Mustapha Siti Nurfatimah, Mohd Shahpudin Ahmad Aizat, Abdul Aziz Bhavaraju, Venkata Muralikrishna Naik, Venkatesh R Zaidi, Zakaria Ahmad Shanwani, Mohd Sidek Mohammad Radzi, Abu Hasan Ankathil, Ravindran |
| author_facet | Mohd Aminudin, Mustapha Siti Nurfatimah, Mohd Shahpudin Ahmad Aizat, Abdul Aziz Bhavaraju, Venkata Muralikrishna Naik, Venkatesh R Zaidi, Zakaria Ahmad Shanwani, Mohd Sidek Mohammad Radzi, Abu Hasan Ankathil, Ravindran |
| author_sort | Mohd Aminudin, Mustapha |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | objective:A case control study was designed to investigate the TNF-a -308 G>A polymorphism allele frequencies and to determine the influence of the polymorphic genotype on sporadic CRC susceptibility risk in Malaysia population.
Material and methods: Peripheral blood samples of 164 normal controls and 161 clinically and histopathhologically confirm CRC patients were genotyped for TNF-a -308 G>A polymorphism employing allele specific PCR. The relative associations of various genotypes with CRC susceptibility risk was determined by calculating Odd ratios. Corresponding Chi-square tests on CRC patients and controls were carried out and 95% confidence interval (95% CI) were determined using fisher exact tests.
Results: On comparing the frequencies of genotypes of patients and controls, the homozygous variant AA was significabtly higher in CRC patients (p=0.030) compared to controls. On investigating the association of the polymorphic genotypes with CRC susceptibility risk, the homozygous variant TNF-a -308AA showed significantly increased risk with OR 2.5842.
Conclusion: Our results suggest that, polymorphic genotype of inflammation response gene TNF-a is significantly associated with CRC susceptibility risk and colud be considered as a high risk variant for CRC predisposition. |
| first_indexed | 2025-11-15T05:54:33Z |
| format | Article |
| id | unimas-706 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T05:54:33Z |
| publishDate | 2011 |
| publisher | Japan International Cultural Exchange Foundation (JICEF), |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-7062019-07-08T00:23:43Z http://ir.unimas.my/id/eprint/706/ Polymorphism in the tumor necrosis factor alpha promoter region and its influence on colorectal cancer predisposition risk in Malaysian population Mohd Aminudin, Mustapha Siti Nurfatimah, Mohd Shahpudin Ahmad Aizat, Abdul Aziz Bhavaraju, Venkata Muralikrishna Naik, Venkatesh R Zaidi, Zakaria Ahmad Shanwani, Mohd Sidek Mohammad Radzi, Abu Hasan Ankathil, Ravindran R Medicine (General) objective:A case control study was designed to investigate the TNF-a -308 G>A polymorphism allele frequencies and to determine the influence of the polymorphic genotype on sporadic CRC susceptibility risk in Malaysia population. Material and methods: Peripheral blood samples of 164 normal controls and 161 clinically and histopathhologically confirm CRC patients were genotyped for TNF-a -308 G>A polymorphism employing allele specific PCR. The relative associations of various genotypes with CRC susceptibility risk was determined by calculating Odd ratios. Corresponding Chi-square tests on CRC patients and controls were carried out and 95% confidence interval (95% CI) were determined using fisher exact tests. Results: On comparing the frequencies of genotypes of patients and controls, the homozygous variant AA was significabtly higher in CRC patients (p=0.030) compared to controls. On investigating the association of the polymorphic genotypes with CRC susceptibility risk, the homozygous variant TNF-a -308AA showed significantly increased risk with OR 2.5842. Conclusion: Our results suggest that, polymorphic genotype of inflammation response gene TNF-a is significantly associated with CRC susceptibility risk and colud be considered as a high risk variant for CRC predisposition. Japan International Cultural Exchange Foundation (JICEF), 2011-12 Article PeerReviewed text en http://ir.unimas.my/id/eprint/706/1/Polymorphism%20in%20the%20tumor%20necrosis%20factor%20alpha%20promoter.pdf Mohd Aminudin, Mustapha and Siti Nurfatimah, Mohd Shahpudin and Ahmad Aizat, Abdul Aziz and Bhavaraju, Venkata Muralikrishna and Naik, Venkatesh R and Zaidi, Zakaria and Ahmad Shanwani, Mohd Sidek and Mohammad Radzi, Abu Hasan and Ankathil, Ravindran (2011) Polymorphism in the tumor necrosis factor alpha promoter region and its influence on colorectal cancer predisposition risk in Malaysian population. International Medical Journal, 18 (4). pp. 275-278. http://www.seronjihou.co.jp/imj/ |
| spellingShingle | R Medicine (General) Mohd Aminudin, Mustapha Siti Nurfatimah, Mohd Shahpudin Ahmad Aizat, Abdul Aziz Bhavaraju, Venkata Muralikrishna Naik, Venkatesh R Zaidi, Zakaria Ahmad Shanwani, Mohd Sidek Mohammad Radzi, Abu Hasan Ankathil, Ravindran Polymorphism in the tumor necrosis factor alpha promoter region and its influence on colorectal cancer predisposition risk in Malaysian population |
| title | Polymorphism in the tumor necrosis factor alpha promoter region and its influence on colorectal cancer predisposition risk in Malaysian population |
| title_full | Polymorphism in the tumor necrosis factor alpha promoter region and its influence on colorectal cancer predisposition risk in Malaysian population |
| title_fullStr | Polymorphism in the tumor necrosis factor alpha promoter region and its influence on colorectal cancer predisposition risk in Malaysian population |
| title_full_unstemmed | Polymorphism in the tumor necrosis factor alpha promoter region and its influence on colorectal cancer predisposition risk in Malaysian population |
| title_short | Polymorphism in the tumor necrosis factor alpha promoter region and its influence on colorectal cancer predisposition risk in Malaysian population |
| title_sort | polymorphism in the tumor necrosis factor alpha promoter region and its influence on colorectal cancer predisposition risk in malaysian population |
| topic | R Medicine (General) |
| url | http://ir.unimas.my/id/eprint/706/ http://ir.unimas.my/id/eprint/706/ http://ir.unimas.my/id/eprint/706/1/Polymorphism%20in%20the%20tumor%20necrosis%20factor%20alpha%20promoter.pdf |