Possible occurrence of a genetic bottleneck in dengue serotype 2 viruses between the 1980 and 1987 epidemic seasons in Bangkok Thailand
Cocirculation of two genetic subtypes of denguc serotype 2 viruses was first observed in the 1980 epidemic season in Thailand. To further delineate the evolutionary history and the contribution of these subtypes to subsequent epidemics, we determined the envelope glycoprotcin gene sequence of 20 den...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The American Society of Tropical Medicine and Hygiene
1997
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/7029/ http://ir.unimas.my/id/eprint/7029/1/Possible%20occurrence%20of%20a%20genetic%20bottleneck%20in%20dengue%20serotype%202%20viruses%20between%20the%201980%20and%201987%20epidemic%20seasons%20in%20Bangkok%20Thailand.pdf |
| _version_ | 1848836044931203072 |
|---|---|
| author | Sittisombut, Nopporn Sistayanarain, Anchalee Cardosa, Mary Jane Salminen, Mika Damrongdachakul, Supaporn Kalayanarooj, Siripen Rojanasuphot, Suntharee Supawadee, Jiraporn Maneekarn, Niwat |
| author_facet | Sittisombut, Nopporn Sistayanarain, Anchalee Cardosa, Mary Jane Salminen, Mika Damrongdachakul, Supaporn Kalayanarooj, Siripen Rojanasuphot, Suntharee Supawadee, Jiraporn Maneekarn, Niwat |
| author_sort | Sittisombut, Nopporn |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Cocirculation of two genetic subtypes of denguc serotype 2 viruses was first observed in the 1980 epidemic season in Thailand. To further delineate the evolutionary history and the contribution of these subtypes to subsequent epidemics, we determined the envelope glycoprotcin gene sequence of 20 dengue serotype 2 viruses isolated from infected patients during 1987 and compared them with those derived from earlier years. Subtype ifia strains represented the majority (18 of 19) of dengue type 2 viruses derived from Bangkok metropolitan area, whereas all three strains from a province in the northeastern region belonged to subtype Ilib, indicating uneven local distni
bution of dengue subtypes within the same year. Three types of sequence variation were identified in both subtypes:
substitutions that were unique to individual strains; substitutions that were shared among all subtype HIa on Ilib
viruses of both the 1980 and 1987 epidemics; and those that were shared only among all subtypes lila or IIlb viruses
of the 1987 epidemic, but were absent from the corresponding subtypes of 1980. While the first and second types of
substitution were indicative of the most recent random mutations and previous mutations that had been fixed in virus
populations, respectively, the third type suggested possible occurrence of a genetic bottleneck and subsequent expan
sion of one or a limited number of subtype lila strains in Bangkok between 1980 and 1987. Immunobbot analysis of
intracellular NS1 antigen with anti-NS1 monocbonal antibodies also revealed antigenic heterogeneity of the NSl
protein that correlated with the subdivision based on envelope protein variation. |
| first_indexed | 2025-11-15T06:17:31Z |
| format | Article |
| id | unimas-7029 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:17:31Z |
| publishDate | 1997 |
| publisher | The American Society of Tropical Medicine and Hygiene |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-70292015-04-14T07:33:56Z http://ir.unimas.my/id/eprint/7029/ Possible occurrence of a genetic bottleneck in dengue serotype 2 viruses between the 1980 and 1987 epidemic seasons in Bangkok Thailand Sittisombut, Nopporn Sistayanarain, Anchalee Cardosa, Mary Jane Salminen, Mika Damrongdachakul, Supaporn Kalayanarooj, Siripen Rojanasuphot, Suntharee Supawadee, Jiraporn Maneekarn, Niwat Q Science (General) QR355 Virology R Medicine (General) Cocirculation of two genetic subtypes of denguc serotype 2 viruses was first observed in the 1980 epidemic season in Thailand. To further delineate the evolutionary history and the contribution of these subtypes to subsequent epidemics, we determined the envelope glycoprotcin gene sequence of 20 dengue serotype 2 viruses isolated from infected patients during 1987 and compared them with those derived from earlier years. Subtype ifia strains represented the majority (18 of 19) of dengue type 2 viruses derived from Bangkok metropolitan area, whereas all three strains from a province in the northeastern region belonged to subtype Ilib, indicating uneven local distni bution of dengue subtypes within the same year. Three types of sequence variation were identified in both subtypes: substitutions that were unique to individual strains; substitutions that were shared among all subtype HIa on Ilib viruses of both the 1980 and 1987 epidemics; and those that were shared only among all subtypes lila or IIlb viruses of the 1987 epidemic, but were absent from the corresponding subtypes of 1980. While the first and second types of substitution were indicative of the most recent random mutations and previous mutations that had been fixed in virus populations, respectively, the third type suggested possible occurrence of a genetic bottleneck and subsequent expan sion of one or a limited number of subtype lila strains in Bangkok between 1980 and 1987. Immunobbot analysis of intracellular NS1 antigen with anti-NS1 monocbonal antibodies also revealed antigenic heterogeneity of the NSl protein that correlated with the subdivision based on envelope protein variation. The American Society of Tropical Medicine and Hygiene 1997 Article PeerReviewed text en http://ir.unimas.my/id/eprint/7029/1/Possible%20occurrence%20of%20a%20genetic%20bottleneck%20in%20dengue%20serotype%202%20viruses%20between%20the%201980%20and%201987%20epidemic%20seasons%20in%20Bangkok%20Thailand.pdf Sittisombut, Nopporn and Sistayanarain, Anchalee and Cardosa, Mary Jane and Salminen, Mika and Damrongdachakul, Supaporn and Kalayanarooj, Siripen and Rojanasuphot, Suntharee and Supawadee, Jiraporn and Maneekarn, Niwat (1997) Possible occurrence of a genetic bottleneck in dengue serotype 2 viruses between the 1980 and 1987 epidemic seasons in Bangkok Thailand. Am J Tropical Medicine and Hygiene, 57 (1). pp. 100-108. ISSN Print ISSN: 0002-9637 http://www.ajtmh.org/content/57/1/100.full.pdf |
| spellingShingle | Q Science (General) QR355 Virology R Medicine (General) Sittisombut, Nopporn Sistayanarain, Anchalee Cardosa, Mary Jane Salminen, Mika Damrongdachakul, Supaporn Kalayanarooj, Siripen Rojanasuphot, Suntharee Supawadee, Jiraporn Maneekarn, Niwat Possible occurrence of a genetic bottleneck in dengue serotype 2 viruses between the 1980 and 1987 epidemic seasons in Bangkok Thailand |
| title | Possible occurrence of a genetic bottleneck in dengue serotype 2 viruses between the 1980 and 1987 epidemic seasons in Bangkok Thailand |
| title_full | Possible occurrence of a genetic bottleneck in dengue serotype 2 viruses between the 1980 and 1987 epidemic seasons in Bangkok Thailand |
| title_fullStr | Possible occurrence of a genetic bottleneck in dengue serotype 2 viruses between the 1980 and 1987 epidemic seasons in Bangkok Thailand |
| title_full_unstemmed | Possible occurrence of a genetic bottleneck in dengue serotype 2 viruses between the 1980 and 1987 epidemic seasons in Bangkok Thailand |
| title_short | Possible occurrence of a genetic bottleneck in dengue serotype 2 viruses between the 1980 and 1987 epidemic seasons in Bangkok Thailand |
| title_sort | possible occurrence of a genetic bottleneck in dengue serotype 2 viruses between the 1980 and 1987 epidemic seasons in bangkok thailand |
| topic | Q Science (General) QR355 Virology R Medicine (General) |
| url | http://ir.unimas.my/id/eprint/7029/ http://ir.unimas.my/id/eprint/7029/ http://ir.unimas.my/id/eprint/7029/1/Possible%20occurrence%20of%20a%20genetic%20bottleneck%20in%20dengue%20serotype%202%20viruses%20between%20the%201980%20and%201987%20epidemic%20seasons%20in%20Bangkok%20Thailand.pdf |