Formaldehyde-Inactivated Whole-Virus Vaccine Protects a Murine Model of Enterovirus 71 Encephalomyelitis against Disease
Enterovirus 71 (EV71) causes childhood hand, foot, and mouth disease and neurological complications, and no vaccines or therapeutic drugs are currently available. Formaldehyde-inactivated whole-virus vaccines derived from EV71 clinical isolates and a mouse-adapted virus (MAV) were tested in a mouse...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2010
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/6978/ http://ir.unimas.my/id/eprint/6978/1/Formaldehyde-Inactivated%20Whole-Virus%20Vaccine%20Protects%20a%20Murine%20Model%20of%20Enterovirus%2071%20Encephalomyelitis%20against%20Disease.pdf |
| _version_ | 1848836036514283520 |
|---|---|
| author | Ong, Kien Chai Devi, Shamala Cardosa, Mary Jane Wong, Kum Thong |
| author_facet | Ong, Kien Chai Devi, Shamala Cardosa, Mary Jane Wong, Kum Thong |
| author_sort | Ong, Kien Chai |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Enterovirus 71 (EV71) causes childhood hand, foot, and mouth disease and neurological complications, and no vaccines or therapeutic drugs are currently available. Formaldehyde-inactivated whole-virus vaccines
derived from EV71 clinical isolates and a mouse-adapted virus (MAV) were tested in a mouse model of EV71
encephalomyelitis. After only two immunizations, given to mice at 1 and 7 days of age, the MAV vaccine
protected mice at 14 days of age from disease. Tissues from immunized mice were negative for virus by viral
culture, reverse transcriptase PCR, immunohistochemistry analysis, and in situ hybridization. Cross-neutralizing
EV71 antibodies to strains with genotypes B3, B4, and C1 to C5 generated in immunized adult mice were
able to passively protect 14-day-old mice from disease. |
| first_indexed | 2025-11-15T06:17:23Z |
| format | Article |
| id | unimas-6978 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:17:23Z |
| publishDate | 2010 |
| publisher | American Society for Microbiology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-69782015-04-10T03:34:01Z http://ir.unimas.my/id/eprint/6978/ Formaldehyde-Inactivated Whole-Virus Vaccine Protects a Murine Model of Enterovirus 71 Encephalomyelitis against Disease Ong, Kien Chai Devi, Shamala Cardosa, Mary Jane Wong, Kum Thong Q Science (General) QR355 Virology R Medicine (General) Enterovirus 71 (EV71) causes childhood hand, foot, and mouth disease and neurological complications, and no vaccines or therapeutic drugs are currently available. Formaldehyde-inactivated whole-virus vaccines derived from EV71 clinical isolates and a mouse-adapted virus (MAV) were tested in a mouse model of EV71 encephalomyelitis. After only two immunizations, given to mice at 1 and 7 days of age, the MAV vaccine protected mice at 14 days of age from disease. Tissues from immunized mice were negative for virus by viral culture, reverse transcriptase PCR, immunohistochemistry analysis, and in situ hybridization. Cross-neutralizing EV71 antibodies to strains with genotypes B3, B4, and C1 to C5 generated in immunized adult mice were able to passively protect 14-day-old mice from disease. American Society for Microbiology 2010-01 Article PeerReviewed text en http://ir.unimas.my/id/eprint/6978/1/Formaldehyde-Inactivated%20Whole-Virus%20Vaccine%20Protects%20a%20Murine%20Model%20of%20Enterovirus%2071%20Encephalomyelitis%20against%20Disease.pdf Ong, Kien Chai and Devi, Shamala and Cardosa, Mary Jane and Wong, Kum Thong (2010) Formaldehyde-Inactivated Whole-Virus Vaccine Protects a Murine Model of Enterovirus 71 Encephalomyelitis against Disease. Journal Of Virology, 84 (1). pp. 661-665. ISSN 0022-538X http://www.ncbi.nlm.nih.gov/pubmed/?term=19864378 |
| spellingShingle | Q Science (General) QR355 Virology R Medicine (General) Ong, Kien Chai Devi, Shamala Cardosa, Mary Jane Wong, Kum Thong Formaldehyde-Inactivated Whole-Virus Vaccine Protects a Murine Model of Enterovirus 71 Encephalomyelitis against Disease |
| title | Formaldehyde-Inactivated Whole-Virus Vaccine Protects a Murine Model of Enterovirus 71 Encephalomyelitis against Disease |
| title_full | Formaldehyde-Inactivated Whole-Virus Vaccine Protects a Murine Model of Enterovirus 71 Encephalomyelitis against Disease |
| title_fullStr | Formaldehyde-Inactivated Whole-Virus Vaccine Protects a Murine Model of Enterovirus 71 Encephalomyelitis against Disease |
| title_full_unstemmed | Formaldehyde-Inactivated Whole-Virus Vaccine Protects a Murine Model of Enterovirus 71 Encephalomyelitis against Disease |
| title_short | Formaldehyde-Inactivated Whole-Virus Vaccine Protects a Murine Model of Enterovirus 71 Encephalomyelitis against Disease |
| title_sort | formaldehyde-inactivated whole-virus vaccine protects a murine model of enterovirus 71 encephalomyelitis against disease |
| topic | Q Science (General) QR355 Virology R Medicine (General) |
| url | http://ir.unimas.my/id/eprint/6978/ http://ir.unimas.my/id/eprint/6978/ http://ir.unimas.my/id/eprint/6978/1/Formaldehyde-Inactivated%20Whole-Virus%20Vaccine%20Protects%20a%20Murine%20Model%20of%20Enterovirus%2071%20Encephalomyelitis%20against%20Disease.pdf |