Novel Multiplex Oligonucleotide-Conjugated Bead Suspension Array for Rapid Identification of Enterovirus 71 Subgenogroups

A high-throughput multiplex bead suspension array was developed for the rapid subgenogrouping of EV71 strains, based on single nucleotide polymorphisms observed within the VP1 region with a high sensitivity as low as 1 PFU. Of 33 viral isolates and 55 clinical samples, all EV71 strains were successf...

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Main Authors: Wu, Y., Tan, E. L., Yeo, A., Chan, K.P., Nishimura, H., Cardosa, M.J, Poh, C.L., Quak, S.H., Chow, Vincent T.
Format: Article
Language:English
Published: American Society for Microbiology 2011
Subjects:
Online Access:http://ir.unimas.my/id/eprint/6939/
http://ir.unimas.my/id/eprint/6939/1/Novel%20Multiplex%20Oligonucleotide-Conjugated%20Bead%20Suspension%20Array%20for%20Rapid%20Identification%20of%20Enterovirus%2071%20Subgenogroups.pdf
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author Wu, Y.
Tan, E. L.
Yeo, A.
Chan, K.P.
Nishimura, H.
Cardosa, M.J
Poh, C.L.
Quak, S.H.
Chow, Vincent T.
author_facet Wu, Y.
Tan, E. L.
Yeo, A.
Chan, K.P.
Nishimura, H.
Cardosa, M.J
Poh, C.L.
Quak, S.H.
Chow, Vincent T.
author_sort Wu, Y.
building UNIMAS Institutional Repository
collection Online Access
description A high-throughput multiplex bead suspension array was developed for the rapid subgenogrouping of EV71 strains, based on single nucleotide polymorphisms observed within the VP1 region with a high sensitivity as low as 1 PFU. Of 33 viral isolates and 55 clinical samples, all EV71 strains were successfully detected and correctly subgenogrouped.
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format Article
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institution Universiti Malaysia Sarawak
institution_category Local University
language English
last_indexed 2025-11-15T06:17:13Z
publishDate 2011
publisher American Society for Microbiology
recordtype eprints
repository_type Digital Repository
spelling unimas-69392015-04-16T02:28:50Z http://ir.unimas.my/id/eprint/6939/ Novel Multiplex Oligonucleotide-Conjugated Bead Suspension Array for Rapid Identification of Enterovirus 71 Subgenogroups Wu, Y. Tan, E. L. Yeo, A. Chan, K.P. Nishimura, H. Cardosa, M.J Poh, C.L. Quak, S.H. Chow, Vincent T. Q Science (General) QR355 Virology R Medicine (General) A high-throughput multiplex bead suspension array was developed for the rapid subgenogrouping of EV71 strains, based on single nucleotide polymorphisms observed within the VP1 region with a high sensitivity as low as 1 PFU. Of 33 viral isolates and 55 clinical samples, all EV71 strains were successfully detected and correctly subgenogrouped. American Society for Microbiology 2011-01 Article PeerReviewed text en http://ir.unimas.my/id/eprint/6939/1/Novel%20Multiplex%20Oligonucleotide-Conjugated%20Bead%20Suspension%20Array%20for%20Rapid%20Identification%20of%20Enterovirus%2071%20Subgenogroups.pdf Wu, Y. and Tan, E. L. and Yeo, A. and Chan, K.P. and Nishimura, H. and Cardosa, M.J and Poh, C.L. and Quak, S.H. and Chow, Vincent T. (2011) Novel Multiplex Oligonucleotide-Conjugated Bead Suspension Array for Rapid Identification of Enterovirus 71 Subgenogroups. Journal of Clinical Microbiology, 49 (1). pp. 419-422. ISSN 0095-1137 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020481/ doi:10.1128/JCM.01871-10
spellingShingle Q Science (General)
QR355 Virology
R Medicine (General)
Wu, Y.
Tan, E. L.
Yeo, A.
Chan, K.P.
Nishimura, H.
Cardosa, M.J
Poh, C.L.
Quak, S.H.
Chow, Vincent T.
Novel Multiplex Oligonucleotide-Conjugated Bead Suspension Array for Rapid Identification of Enterovirus 71 Subgenogroups
title Novel Multiplex Oligonucleotide-Conjugated Bead Suspension Array for Rapid Identification of Enterovirus 71 Subgenogroups
title_full Novel Multiplex Oligonucleotide-Conjugated Bead Suspension Array for Rapid Identification of Enterovirus 71 Subgenogroups
title_fullStr Novel Multiplex Oligonucleotide-Conjugated Bead Suspension Array for Rapid Identification of Enterovirus 71 Subgenogroups
title_full_unstemmed Novel Multiplex Oligonucleotide-Conjugated Bead Suspension Array for Rapid Identification of Enterovirus 71 Subgenogroups
title_short Novel Multiplex Oligonucleotide-Conjugated Bead Suspension Array for Rapid Identification of Enterovirus 71 Subgenogroups
title_sort novel multiplex oligonucleotide-conjugated bead suspension array for rapid identification of enterovirus 71 subgenogroups
topic Q Science (General)
QR355 Virology
R Medicine (General)
url http://ir.unimas.my/id/eprint/6939/
http://ir.unimas.my/id/eprint/6939/
http://ir.unimas.my/id/eprint/6939/
http://ir.unimas.my/id/eprint/6939/1/Novel%20Multiplex%20Oligonucleotide-Conjugated%20Bead%20Suspension%20Array%20for%20Rapid%20Identification%20of%20Enterovirus%2071%20Subgenogroups.pdf