Enterovirus 71 encephalomyelitis and Japanese encephalitis can be distinguished by topographic distribution of inflammation and specific intraneuronal detection of viral antigen and RNA
Aims: To investigate if two important epidemic viral encephalitis in children, Enterovirus 71 (EV71)e ncephalomyelitis and Japanese encephalitis (JE) whose clinical and pathological features may be nonspecific and overlapping, could be distinguished. Methods: Tissue sections from the central ne...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
British Neuropathological Society
2012
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/6697/ http://ir.unimas.my/id/eprint/6697/1/Enterovirus.pdf |
| _version_ | 1848835975199850496 |
|---|---|
| author | Wong, K.T. Ng, K.Y. Ong, K.C. Ng, W.F Shankar, S.K. Mahadevan, A. Radotra, B. Su, I.J. Lau, G. Ling, A.E. Chan, K.P. Macorelles, P. Vallet, S. Cardosa, Mary Jane Desai, A. Ravi, V. Nagata, N. Shimizu, H. Takasaki, T. |
| author_facet | Wong, K.T. Ng, K.Y. Ong, K.C. Ng, W.F Shankar, S.K. Mahadevan, A. Radotra, B. Su, I.J. Lau, G. Ling, A.E. Chan, K.P. Macorelles, P. Vallet, S. Cardosa, Mary Jane Desai, A. Ravi, V. Nagata, N. Shimizu, H. Takasaki, T. |
| author_sort | Wong, K.T. |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Aims: To investigate if two important epidemic viral
encephalitis in children, Enterovirus 71 (EV71)e ncephalomyelitis and Japanese encephalitis (JE) whose clinical and pathological features may be nonspecific and overlapping, could be distinguished.
Methods: Tissue sections from the central nervous system of infected cases were examined by light microscopy, immunohistochemistry and in situ hybridization. Results: All 13 cases of EV71 encephalomyelitis collected from Asia and France invariably showed stereotyped distribution of inflammation in the spinal cord, brainstem, hypothalamus, cerebellar dentate nucleus and, to a lesser extent, cerebral cortex and meninges. Anterior pons, corpus striatum, thalamus, temporal lobe, hippocampus and cerebellar cortex were always uninflamed. In contrast, the eight JE cases studied showed inflammation involving most neuronal areas of the central nervous system, including the areas that were uninflamed in EV71 encephalomyelitis. Lesions in both infections were nonspecific, consisting of perivascular and parenchymal infiltration by inflammatory cells,
oedematous/necrolytic areas, microglial nodules and neuronophagia. Viral inclusions were absent.
Conclusions:
Immunohistochemistry and in situ hybridization assays
were useful to identify the causative virus, localizing viral antigens and RNA, respectively, almost exclusively to neurones. The stereotyped distribution of inflammatory
lesions in EV71 encephalomyelitis appears to be very
useful to help distinguish it from JE. |
| first_indexed | 2025-11-15T06:16:24Z |
| format | Article |
| id | unimas-6697 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:16:24Z |
| publishDate | 2012 |
| publisher | British Neuropathological Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-66972021-06-05T05:40:36Z http://ir.unimas.my/id/eprint/6697/ Enterovirus 71 encephalomyelitis and Japanese encephalitis can be distinguished by topographic distribution of inflammation and specific intraneuronal detection of viral antigen and RNA Wong, K.T. Ng, K.Y. Ong, K.C. Ng, W.F Shankar, S.K. Mahadevan, A. Radotra, B. Su, I.J. Lau, G. Ling, A.E. Chan, K.P. Macorelles, P. Vallet, S. Cardosa, Mary Jane Desai, A. Ravi, V. Nagata, N. Shimizu, H. Takasaki, T. Q Science (General) QR355 Virology R Medicine (General) Aims: To investigate if two important epidemic viral encephalitis in children, Enterovirus 71 (EV71)e ncephalomyelitis and Japanese encephalitis (JE) whose clinical and pathological features may be nonspecific and overlapping, could be distinguished. Methods: Tissue sections from the central nervous system of infected cases were examined by light microscopy, immunohistochemistry and in situ hybridization. Results: All 13 cases of EV71 encephalomyelitis collected from Asia and France invariably showed stereotyped distribution of inflammation in the spinal cord, brainstem, hypothalamus, cerebellar dentate nucleus and, to a lesser extent, cerebral cortex and meninges. Anterior pons, corpus striatum, thalamus, temporal lobe, hippocampus and cerebellar cortex were always uninflamed. In contrast, the eight JE cases studied showed inflammation involving most neuronal areas of the central nervous system, including the areas that were uninflamed in EV71 encephalomyelitis. Lesions in both infections were nonspecific, consisting of perivascular and parenchymal infiltration by inflammatory cells, oedematous/necrolytic areas, microglial nodules and neuronophagia. Viral inclusions were absent. Conclusions: Immunohistochemistry and in situ hybridization assays were useful to identify the causative virus, localizing viral antigens and RNA, respectively, almost exclusively to neurones. The stereotyped distribution of inflammatory lesions in EV71 encephalomyelitis appears to be very useful to help distinguish it from JE. British Neuropathological Society 2012 Article PeerReviewed text en http://ir.unimas.my/id/eprint/6697/1/Enterovirus.pdf Wong, K.T. and Ng, K.Y. and Ong, K.C. and Ng, W.F and Shankar, S.K. and Mahadevan, A. and Radotra, B. and Su, I.J. and Lau, G. and Ling, A.E. and Chan, K.P. and Macorelles, P. and Vallet, S. and Cardosa, Mary Jane and Desai, A. and Ravi, V. and Nagata, N. and Shimizu, H. and Takasaki, T. (2012) Enterovirus 71 encephalomyelitis and Japanese encephalitis can be distinguished by topographic distribution of inflammation and specific intraneuronal detection of viral antigen and RNA. Neuropathology and Applied Neurobiology, 38. pp. 443-453. ISSN 1365-2990 https://pubmed.ncbi.nlm.nih.gov/22236252/ doi: 10.1111/j.1365-2990.2011.01247.x |
| spellingShingle | Q Science (General) QR355 Virology R Medicine (General) Wong, K.T. Ng, K.Y. Ong, K.C. Ng, W.F Shankar, S.K. Mahadevan, A. Radotra, B. Su, I.J. Lau, G. Ling, A.E. Chan, K.P. Macorelles, P. Vallet, S. Cardosa, Mary Jane Desai, A. Ravi, V. Nagata, N. Shimizu, H. Takasaki, T. Enterovirus 71 encephalomyelitis and Japanese encephalitis can be distinguished by topographic distribution of inflammation and specific intraneuronal detection of viral antigen and RNA |
| title | Enterovirus 71 encephalomyelitis and Japanese encephalitis can be distinguished by topographic distribution of inflammation and specific intraneuronal detection of viral antigen and RNA |
| title_full | Enterovirus 71 encephalomyelitis and Japanese encephalitis can be distinguished by topographic distribution of inflammation and specific intraneuronal detection of viral antigen and RNA |
| title_fullStr | Enterovirus 71 encephalomyelitis and Japanese encephalitis can be distinguished by topographic distribution of inflammation and specific intraneuronal detection of viral antigen and RNA |
| title_full_unstemmed | Enterovirus 71 encephalomyelitis and Japanese encephalitis can be distinguished by topographic distribution of inflammation and specific intraneuronal detection of viral antigen and RNA |
| title_short | Enterovirus 71 encephalomyelitis and Japanese encephalitis can be distinguished by topographic distribution of inflammation and specific intraneuronal detection of viral antigen and RNA |
| title_sort | enterovirus 71 encephalomyelitis and japanese encephalitis can be distinguished by topographic distribution of inflammation and specific intraneuronal detection of viral antigen and rna |
| topic | Q Science (General) QR355 Virology R Medicine (General) |
| url | http://ir.unimas.my/id/eprint/6697/ http://ir.unimas.my/id/eprint/6697/ http://ir.unimas.my/id/eprint/6697/ http://ir.unimas.my/id/eprint/6697/1/Enterovirus.pdf |