Preliminary morphological and molecular study on ghost crabs from Satang Island and Sematan, Sarawak

Ocypode ceratophthalmus commonly known as ghost crabs could be found abundance around the sandy beaches area worldwide. They act as bio-indicator for clean sandy beaches as well as pest to turtles conservation sites. Several studies related to O.ceratophthalmus on ecology, behaviour and morphology...

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Main Author: Uzma Hazwani, bt Arif
Format: Final Year Project Report / IMRAD
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2011
Subjects:
Online Access:http://ir.unimas.my/id/eprint/6432/
http://ir.unimas.my/id/eprint/6432/4/Uzma%28fulltext%29.pdf
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author Uzma Hazwani, bt Arif
author_facet Uzma Hazwani, bt Arif
author_sort Uzma Hazwani, bt Arif
building UNIMAS Institutional Repository
collection Online Access
description Ocypode ceratophthalmus commonly known as ghost crabs could be found abundance around the sandy beaches area worldwide. They act as bio-indicator for clean sandy beaches as well as pest to turtles conservation sites. Several studies related to O.ceratophthalmus on ecology, behaviour and morphology had been carried out outside Malaysia but no study had been carried out in Sarawak beaches. Therefore, this study is designed to document the morphological characteristics of the ghost crabs and to access the genetic diversity among ghost crabs from Satang Island and Sematan area. Modified CTAB protocol was used to obtain the total genomic DNA , followed by PCR-RAPD technique using primer OPA-01. In addition, weight, carapace length, carapace width, chelipeds height and chelipeds length were recorded. PCR-RAPD profile using OPA-01 primer was successfully generated 86.36% polymorphism. For male ghost crabs, there was no significant different (T-test, p>0.05) between Satang Island and Sematan sample in term of carapace width, carapace length, chelipeds height, chelipeds width and weight. For female ghost crabs, there only have significant different (T-test, p>0.05) between Satang Island and Sematan sample in term of carapace length and carapace width while there is no significant different (T-test, p<0.05) in terms of chelipeds height, chelipeds length and weight.
first_indexed 2025-11-15T06:15:28Z
format Final Year Project Report / IMRAD
id unimas-6432
institution Universiti Malaysia Sarawak
institution_category Local University
language English
last_indexed 2025-11-15T06:15:28Z
publishDate 2011
publisher Universiti Malaysia Sarawak, (UNIMAS)
recordtype eprints
repository_type Digital Repository
spelling unimas-64322024-09-12T08:00:08Z http://ir.unimas.my/id/eprint/6432/ Preliminary morphological and molecular study on ghost crabs from Satang Island and Sematan, Sarawak Uzma Hazwani, bt Arif QE Geology SH Aquaculture. Fisheries. Angling Ocypode ceratophthalmus commonly known as ghost crabs could be found abundance around the sandy beaches area worldwide. They act as bio-indicator for clean sandy beaches as well as pest to turtles conservation sites. Several studies related to O.ceratophthalmus on ecology, behaviour and morphology had been carried out outside Malaysia but no study had been carried out in Sarawak beaches. Therefore, this study is designed to document the morphological characteristics of the ghost crabs and to access the genetic diversity among ghost crabs from Satang Island and Sematan area. Modified CTAB protocol was used to obtain the total genomic DNA , followed by PCR-RAPD technique using primer OPA-01. In addition, weight, carapace length, carapace width, chelipeds height and chelipeds length were recorded. PCR-RAPD profile using OPA-01 primer was successfully generated 86.36% polymorphism. For male ghost crabs, there was no significant different (T-test, p>0.05) between Satang Island and Sematan sample in term of carapace width, carapace length, chelipeds height, chelipeds width and weight. For female ghost crabs, there only have significant different (T-test, p>0.05) between Satang Island and Sematan sample in term of carapace length and carapace width while there is no significant different (T-test, p<0.05) in terms of chelipeds height, chelipeds length and weight. Universiti Malaysia Sarawak, (UNIMAS) 2011 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/6432/4/Uzma%28fulltext%29.pdf Uzma Hazwani, bt Arif (2011) Preliminary morphological and molecular study on ghost crabs from Satang Island and Sematan, Sarawak. [Final Year Project Report / IMRAD] (Unpublished)
spellingShingle QE Geology
SH Aquaculture. Fisheries. Angling
Uzma Hazwani, bt Arif
Preliminary morphological and molecular study on ghost crabs from Satang Island and Sematan, Sarawak
title Preliminary morphological and molecular study on ghost crabs from Satang Island and Sematan, Sarawak
title_full Preliminary morphological and molecular study on ghost crabs from Satang Island and Sematan, Sarawak
title_fullStr Preliminary morphological and molecular study on ghost crabs from Satang Island and Sematan, Sarawak
title_full_unstemmed Preliminary morphological and molecular study on ghost crabs from Satang Island and Sematan, Sarawak
title_short Preliminary morphological and molecular study on ghost crabs from Satang Island and Sematan, Sarawak
title_sort preliminary morphological and molecular study on ghost crabs from satang island and sematan, sarawak
topic QE Geology
SH Aquaculture. Fisheries. Angling
url http://ir.unimas.my/id/eprint/6432/
http://ir.unimas.my/id/eprint/6432/4/Uzma%28fulltext%29.pdf