Genetic diversity of saltwater crocodiles (Crocodylus porosus) from Sarawak using microsatellite approach
Crocodylus porosus (saltwater crocodile) locally known as Bujang Senang is the common crocodile found in Sarawak. There were several genetic studies related to C. porosus in Sarawak that have been conducted, however relationships between C. porosus in Sarawak remained unresolved. Therefore, this stu...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, (UNIMAS)
2011
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| Online Access: | http://ir.unimas.my/id/eprint/6422/ http://ir.unimas.my/id/eprint/6422/8/Genetic%20Diversity%20of%20Saltwater%20Crocodiles%20%28Crocodylus%20porosus%29%20%28OCR%29.pdf |
| _version_ | 1848835913892757504 |
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| author | Nor Yasmin, Kasim |
| author_facet | Nor Yasmin, Kasim |
| author_sort | Nor Yasmin, Kasim |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Crocodylus porosus (saltwater crocodile) locally known as Bujang Senang is the common crocodile found in Sarawak. There were several genetic studies related to C. porosus in Sarawak that have been conducted, however relationships between C. porosus in Sarawak remained unresolved. Therefore, this study is aimed to assess genetic diversity of C. porosus obtained from different locations in Sarawak using microsatellite approach. Available C. porosus tissues samples were subjected to total genomic DNA extraction using modified CTAB method, Polymerase Chain Reaction – Simple Sequence Repeats (PCR-SSRs) and data analysis. Microsatellite loci for C. porosus from Sibu, Miri and Bako of approximately 115 to 122 base pairs (bp) were successfully obtained during this study. C. porosus from Sibu showed repeat motifs of (GT)22 (GA)5, Miri (GT)21 (GA)5 and Bako (GT)20 (GA)5. Phylogenetic tree construction using Unweighted Pair Group with Arithmetic Averages (UPGMA) and Maximum Parsimony (MP) methods revealed monophyletic grouping of C. porosus. |
| first_indexed | 2025-11-15T06:15:26Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-6422 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:15:26Z |
| publishDate | 2011 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-64222023-11-29T03:52:57Z http://ir.unimas.my/id/eprint/6422/ Genetic diversity of saltwater crocodiles (Crocodylus porosus) from Sarawak using microsatellite approach Nor Yasmin, Kasim QL Zoology SH Aquaculture. Fisheries. Angling Crocodylus porosus (saltwater crocodile) locally known as Bujang Senang is the common crocodile found in Sarawak. There were several genetic studies related to C. porosus in Sarawak that have been conducted, however relationships between C. porosus in Sarawak remained unresolved. Therefore, this study is aimed to assess genetic diversity of C. porosus obtained from different locations in Sarawak using microsatellite approach. Available C. porosus tissues samples were subjected to total genomic DNA extraction using modified CTAB method, Polymerase Chain Reaction – Simple Sequence Repeats (PCR-SSRs) and data analysis. Microsatellite loci for C. porosus from Sibu, Miri and Bako of approximately 115 to 122 base pairs (bp) were successfully obtained during this study. C. porosus from Sibu showed repeat motifs of (GT)22 (GA)5, Miri (GT)21 (GA)5 and Bako (GT)20 (GA)5. Phylogenetic tree construction using Unweighted Pair Group with Arithmetic Averages (UPGMA) and Maximum Parsimony (MP) methods revealed monophyletic grouping of C. porosus. Universiti Malaysia Sarawak, (UNIMAS) 2011 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/6422/8/Genetic%20Diversity%20of%20Saltwater%20Crocodiles%20%28Crocodylus%20porosus%29%20%28OCR%29.pdf Nor Yasmin, Kasim (2011) Genetic diversity of saltwater crocodiles (Crocodylus porosus) from Sarawak using microsatellite approach. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | QL Zoology SH Aquaculture. Fisheries. Angling Nor Yasmin, Kasim Genetic diversity of saltwater crocodiles (Crocodylus porosus) from Sarawak using microsatellite approach |
| title | Genetic diversity of saltwater crocodiles (Crocodylus porosus) from Sarawak using microsatellite approach |
| title_full | Genetic diversity of saltwater crocodiles (Crocodylus porosus) from Sarawak using microsatellite approach |
| title_fullStr | Genetic diversity of saltwater crocodiles (Crocodylus porosus) from Sarawak using microsatellite approach |
| title_full_unstemmed | Genetic diversity of saltwater crocodiles (Crocodylus porosus) from Sarawak using microsatellite approach |
| title_short | Genetic diversity of saltwater crocodiles (Crocodylus porosus) from Sarawak using microsatellite approach |
| title_sort | genetic diversity of saltwater crocodiles (crocodylus porosus) from sarawak using microsatellite approach |
| topic | QL Zoology SH Aquaculture. Fisheries. Angling |
| url | http://ir.unimas.my/id/eprint/6422/ http://ir.unimas.my/id/eprint/6422/8/Genetic%20Diversity%20of%20Saltwater%20Crocodiles%20%28Crocodylus%20porosus%29%20%28OCR%29.pdf |