The effects of sucrose concentrations on somatic embryogenesis of limau manis (citrus sinensis L.)
Citrus sinensis or Limau Manis belongs to the family of Rutaceae.It is a species among most cultivated worldwide. This species can be cultivated sexually or asexually. Despite of it, the reproduction by seeds for this species leads to many problems. The species is susceptible to pathogens and dise...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, UNIMAS
2012
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| Online Access: | http://ir.unimas.my/id/eprint/6280/ http://ir.unimas.my/id/eprint/6280/8/SITI%20NURHAZLINDA.pdf |
| Summary: | Citrus sinensis or Limau Manis belongs to the family of Rutaceae.It is a species among most cultivated
worldwide. This species can be cultivated sexually or asexually. Despite of it, the reproduction by seeds for this
species leads to many problems. The species is susceptible to pathogens and diseases too. Somatic embryogenesis
is one of the efficient methods in plants tissue culture for mass propagation within a short period of time. Limau
Manis seeds were successfully germinated axenically in 2.0 mg/L BAP. The node and leaf segments were
purposely used in this experiment. Seven different sucrose concentrations were used to identify its effects
towards the development of callus and somatic embryos. Limau Manis callus were gave different growth
performance towards callus induction media, 20% coconut water, 2.0 mg/L of 2,4-D and different sucrose
concentration. No somatic embryo Was developed when callus were cultured on embryos induction media, 1.5
mg L-1 Kinetin + 0.1 mg L-1 NAA even after 3 months of the culture established. |
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