Screening and characterization of methane producing bacteria from sago waste
A study was done to determine the presence of methane producing bacteria, methanogens, in sago wastes. The bacteria was cultured in sealed bottles containing Luria broth and the sago wastes. One of the bottles was incubated at 30°C and the other one at room temperature. The bacteria were then inoc...
| Main Author: | |
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2012
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/6252/ http://ir.unimas.my/id/eprint/6252/11/Dahalia%20Anak%20Jerim%20ft.pdf |
| _version_ | 1848835874967519232 |
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| author | Dahalia, anak Jerim |
| author_facet | Dahalia, anak Jerim |
| author_sort | Dahalia, anak Jerim |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | A study was done to determine the presence of methane producing bacteria, methanogens, in sago wastes. The
bacteria was cultured in sealed bottles containing Luria broth and the sago wastes. One of the bottles was
incubated at 30°C and the other one at room temperature. The bacteria were then inoculated and culutured in LB
agar plates for several days. The growth of bacteria was being observed. The bacteria colonies have white
colonies. Through staining, the bacteria was Gram positive bacteria and microscopic observation determined the
characteristic of the bacteria showed to be rod shaped. Further study was done by using DNA extraction to
determine the presence of 16S ribosomal bacteria. The sample from bacteria incubated in 30°C temperature
showed the appearance of bands during AGE. After running the AGE, the template from extraction was used for
PCR processes. The primer ME1, ME2, mmox f, mmox r, 27, 1525, mb 1007, mc 1005, mm 1007 and ms 1020
were used as forward and reverse primers during PCR processes. The AGE process of PCR products showed the
appearanced of bands in the primer samples except in sample with primer mmoX. After the PCR process, the
samples were sent to 1st Base Sequencing Company for direct sequencing. |
| first_indexed | 2025-11-15T06:14:49Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-6252 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:14:49Z |
| publishDate | 2012 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-62522023-02-27T04:07:18Z http://ir.unimas.my/id/eprint/6252/ Screening and characterization of methane producing bacteria from sago waste Dahalia, anak Jerim Q Science (General) QR Microbiology A study was done to determine the presence of methane producing bacteria, methanogens, in sago wastes. The bacteria was cultured in sealed bottles containing Luria broth and the sago wastes. One of the bottles was incubated at 30°C and the other one at room temperature. The bacteria were then inoculated and culutured in LB agar plates for several days. The growth of bacteria was being observed. The bacteria colonies have white colonies. Through staining, the bacteria was Gram positive bacteria and microscopic observation determined the characteristic of the bacteria showed to be rod shaped. Further study was done by using DNA extraction to determine the presence of 16S ribosomal bacteria. The sample from bacteria incubated in 30°C temperature showed the appearance of bands during AGE. After running the AGE, the template from extraction was used for PCR processes. The primer ME1, ME2, mmox f, mmox r, 27, 1525, mb 1007, mc 1005, mm 1007 and ms 1020 were used as forward and reverse primers during PCR processes. The AGE process of PCR products showed the appearanced of bands in the primer samples except in sample with primer mmoX. After the PCR process, the samples were sent to 1st Base Sequencing Company for direct sequencing. Universiti Malaysia Sarawak, (UNIMAS) 2012 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/6252/11/Dahalia%20Anak%20Jerim%20ft.pdf Dahalia, anak Jerim (2012) Screening and characterization of methane producing bacteria from sago waste. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | Q Science (General) QR Microbiology Dahalia, anak Jerim Screening and characterization of methane producing bacteria from sago waste |
| title | Screening and characterization of methane producing bacteria from sago waste |
| title_full | Screening and characterization of methane producing bacteria from sago waste |
| title_fullStr | Screening and characterization of methane producing bacteria from sago waste |
| title_full_unstemmed | Screening and characterization of methane producing bacteria from sago waste |
| title_short | Screening and characterization of methane producing bacteria from sago waste |
| title_sort | screening and characterization of methane producing bacteria from sago waste |
| topic | Q Science (General) QR Microbiology |
| url | http://ir.unimas.my/id/eprint/6252/ http://ir.unimas.my/id/eprint/6252/11/Dahalia%20Anak%20Jerim%20ft.pdf |