Lactic acid production using enteroccos faecalis in hydrolyzed sago starch by repeated batch fermentation
The production of lactic acid (LA) by utilizing the raw carbon source of hydrolyzed sago starch (HSS) using newly isolate Enterococcus faecalis strain by repeated batch fermentation (RBF) methodology was investigated in this study. The aim was to investigate if E. faecalis is able to produce LA a...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, UNIMAS
2012
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| Online Access: | http://ir.unimas.my/id/eprint/6207/ http://ir.unimas.my/id/eprint/6207/3/Afri%20Shafrulniiza%20ft.pdf |
| _version_ | 1848835862413967360 |
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| author | Afri Shafrulniiza, Binti Shafriil |
| author_facet | Afri Shafrulniiza, Binti Shafriil |
| author_sort | Afri Shafrulniiza, Binti Shafriil |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | The production of lactic acid (LA) by utilizing the raw carbon source of hydrolyzed sago
starch (HSS) using newly isolate Enterococcus faecalis strain by repeated batch
fermentation (RBF) methodology was investigated in this study. The aim was to
investigate if E. faecalis is able to produce LA at high concentration for a possible
industrial application. The fermentation was carried out at pH 6.5 by addition of sodium
hydroxide, controlled at 40°C and agitated at 250 rpm. HSS concentration was varied
ranging from 30 to 100 g/l to as consequence of the increasing of biomass. The results
showed that the production of LA is the highest during the fourth cycle providing high
concentration of HSS, 100 g/l at the beginning of the cycle, despite low volumetric
productivity and yield. However, it was found that the optimum HSS concentration for the
LA production using this strain is at 80 g/l which produced volumetric productivity and
yield of 1.27 g/lh and 0.54 g/g, respectively. In overall, the total LA production throughout
the 7 cycles of RBF is 130.53 g/l with volumetric productivity and yield of 1.02 g/lh and
0.54 g/g, respectively. |
| first_indexed | 2025-11-15T06:14:37Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-6207 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:14:37Z |
| publishDate | 2012 |
| publisher | Universiti Malaysia Sarawak, UNIMAS |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-62072024-02-05T01:42:48Z http://ir.unimas.my/id/eprint/6207/ Lactic acid production using enteroccos faecalis in hydrolyzed sago starch by repeated batch fermentation Afri Shafrulniiza, Binti Shafriil GE Environmental Sciences QH Natural history The production of lactic acid (LA) by utilizing the raw carbon source of hydrolyzed sago starch (HSS) using newly isolate Enterococcus faecalis strain by repeated batch fermentation (RBF) methodology was investigated in this study. The aim was to investigate if E. faecalis is able to produce LA at high concentration for a possible industrial application. The fermentation was carried out at pH 6.5 by addition of sodium hydroxide, controlled at 40°C and agitated at 250 rpm. HSS concentration was varied ranging from 30 to 100 g/l to as consequence of the increasing of biomass. The results showed that the production of LA is the highest during the fourth cycle providing high concentration of HSS, 100 g/l at the beginning of the cycle, despite low volumetric productivity and yield. However, it was found that the optimum HSS concentration for the LA production using this strain is at 80 g/l which produced volumetric productivity and yield of 1.27 g/lh and 0.54 g/g, respectively. In overall, the total LA production throughout the 7 cycles of RBF is 130.53 g/l with volumetric productivity and yield of 1.02 g/lh and 0.54 g/g, respectively. Universiti Malaysia Sarawak, UNIMAS 2012 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/6207/3/Afri%20Shafrulniiza%20ft.pdf Afri Shafrulniiza, Binti Shafriil (2012) Lactic acid production using enteroccos faecalis in hydrolyzed sago starch by repeated batch fermentation. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | GE Environmental Sciences QH Natural history Afri Shafrulniiza, Binti Shafriil Lactic acid production using enteroccos faecalis in hydrolyzed sago starch by repeated batch fermentation |
| title | Lactic acid production using enteroccos faecalis in hydrolyzed sago starch by repeated batch fermentation |
| title_full | Lactic acid production using enteroccos faecalis in hydrolyzed sago starch by repeated batch fermentation |
| title_fullStr | Lactic acid production using enteroccos faecalis in hydrolyzed sago starch by repeated batch fermentation |
| title_full_unstemmed | Lactic acid production using enteroccos faecalis in hydrolyzed sago starch by repeated batch fermentation |
| title_short | Lactic acid production using enteroccos faecalis in hydrolyzed sago starch by repeated batch fermentation |
| title_sort | lactic acid production using enteroccos faecalis in hydrolyzed sago starch by repeated batch fermentation |
| topic | GE Environmental Sciences QH Natural history |
| url | http://ir.unimas.my/id/eprint/6207/ http://ir.unimas.my/id/eprint/6207/3/Afri%20Shafrulniiza%20ft.pdf |