Lactic Acid production using alkaliphile lactic acid bacterium
Batch fermentation of glucose to lactic acid was conducted using new isolated bacteria from laboratory of Biofuel R&D (UNIMAS) at alkali pH of 9 and 10. Commercial glucose was used to produce lactic acid and the effect of alkali pH on biomass, lactic acid production, and presence of contamina...
| Main Author: | |
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, UNIMAS
2012
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/6206/ http://ir.unimas.my/id/eprint/6206/1/Amirul%20Bin%20Mahmud%20ft.pdf |
| _version_ | 1848835862099394560 |
|---|---|
| author | Amirul, Bin Mahmud |
| author_facet | Amirul, Bin Mahmud |
| author_sort | Amirul, Bin Mahmud |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Batch fermentation of glucose to lactic acid was conducted using new isolated bacteria from
laboratory of Biofuel R&D (UNIMAS) at alkali pH of 9 and 10. Commercial glucose was
used to produce lactic acid and the effect of alkali pH on biomass, lactic acid production, and
presence of contamination were investigated. Batch fermentation system was done at 37 °C
and agitated at 250 rpm with controlled pH environment. Fermentation at pH 9 gave high
production biomass and lactic acid of about 1.80 g/L and 45.21 g/L while less production is
shown at pH 10 with 1.27 g/L and 42.66 g/L. Spread plate of both fermentation show growth
only single colony which no contamination. Consequently, it shows that high pH affect cell
growth, lactic acid production and prevents contamination. Complete fermentation at pH 9
show is more favourable with high rate production of lactic acid in 42 h compared to at pH 10
in 72 h. |
| first_indexed | 2025-11-15T06:14:36Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-6206 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:14:36Z |
| publishDate | 2012 |
| publisher | Universiti Malaysia Sarawak, UNIMAS |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-62062023-02-22T05:20:30Z http://ir.unimas.my/id/eprint/6206/ Lactic Acid production using alkaliphile lactic acid bacterium Amirul, Bin Mahmud GE Environmental Sciences Q Science (General) QH301 Biology Batch fermentation of glucose to lactic acid was conducted using new isolated bacteria from laboratory of Biofuel R&D (UNIMAS) at alkali pH of 9 and 10. Commercial glucose was used to produce lactic acid and the effect of alkali pH on biomass, lactic acid production, and presence of contamination were investigated. Batch fermentation system was done at 37 °C and agitated at 250 rpm with controlled pH environment. Fermentation at pH 9 gave high production biomass and lactic acid of about 1.80 g/L and 45.21 g/L while less production is shown at pH 10 with 1.27 g/L and 42.66 g/L. Spread plate of both fermentation show growth only single colony which no contamination. Consequently, it shows that high pH affect cell growth, lactic acid production and prevents contamination. Complete fermentation at pH 9 show is more favourable with high rate production of lactic acid in 42 h compared to at pH 10 in 72 h. Universiti Malaysia Sarawak, UNIMAS 2012 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/6206/1/Amirul%20Bin%20Mahmud%20ft.pdf Amirul, Bin Mahmud (2012) Lactic Acid production using alkaliphile lactic acid bacterium. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | GE Environmental Sciences Q Science (General) QH301 Biology Amirul, Bin Mahmud Lactic Acid production using alkaliphile lactic acid bacterium |
| title | Lactic Acid production using alkaliphile lactic acid bacterium |
| title_full | Lactic Acid production using alkaliphile lactic acid bacterium |
| title_fullStr | Lactic Acid production using alkaliphile lactic acid bacterium |
| title_full_unstemmed | Lactic Acid production using alkaliphile lactic acid bacterium |
| title_short | Lactic Acid production using alkaliphile lactic acid bacterium |
| title_sort | lactic acid production using alkaliphile lactic acid bacterium |
| topic | GE Environmental Sciences Q Science (General) QH301 Biology |
| url | http://ir.unimas.my/id/eprint/6206/ http://ir.unimas.my/id/eprint/6206/1/Amirul%20Bin%20Mahmud%20ft.pdf |