Isolation and nucleotide sequence survey of the ribosomal protein gene (RPL27) from human nasopharyngeal-derived cell lines
This study is about isolation and nucleotide sequence survey of RPL27 gene in human nasopharyngeal cell line. Literature has been stated that RPL27 gene found to be downregulated in nasopharyngeal carcinoma cases. The ribosomal protein L27 gene has been used in expression studies of human nasopha...
| Main Author: | |
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, UNIMAS
2012
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/6200/ http://ir.unimas.my/id/eprint/6200/1/BUWANESWARI%20ft.pdf |
| _version_ | 1848835860707934208 |
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| author | Buwaneswari, Nagayan |
| author_facet | Buwaneswari, Nagayan |
| author_sort | Buwaneswari, Nagayan |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | This study is about isolation and nucleotide sequence survey of RPL27 gene in human
nasopharyngeal cell line. Literature has been stated that RPL27 gene found to be
downregulated in nasopharyngeal carcinoma cases. The ribosomal protein L27 gene has been
used in expression studies of human nasopharyngeal carcinoma but there is no studies related
to the nucleotide sequence analysis of RPL27 gene in nasopharyngeal carcinoma cases.
Moreover, most of the studies that have been conducted using this gene are using tissue rather
than cell line as sample. The main aim of the study is to isolate and study the nucleotide
sequence of RPL27 gene from human nasopharyngeal carcinoma and normal cell line. The
methods used in this study are isolation of total RNA using TRIzol method, reverse
transcriptase polymerase chain reaction, and amplification of DNA, purifying, sequencing and
finally analyzing the nucleotide sequence of the RPL27 gene using bioinformatics. From this
study, it can be found that, there is no nucleotide variation occurs due to mutation in the
region of study in RPL27 gene derived from both nasopharyngeal normal and carcinoma cell
line. |
| first_indexed | 2025-11-15T06:14:35Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-6200 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:14:35Z |
| publishDate | 2012 |
| publisher | Universiti Malaysia Sarawak, UNIMAS |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-62002023-02-14T07:02:54Z http://ir.unimas.my/id/eprint/6200/ Isolation and nucleotide sequence survey of the ribosomal protein gene (RPL27) from human nasopharyngeal-derived cell lines Buwaneswari, Nagayan Q Science (General) QH301 Biology This study is about isolation and nucleotide sequence survey of RPL27 gene in human nasopharyngeal cell line. Literature has been stated that RPL27 gene found to be downregulated in nasopharyngeal carcinoma cases. The ribosomal protein L27 gene has been used in expression studies of human nasopharyngeal carcinoma but there is no studies related to the nucleotide sequence analysis of RPL27 gene in nasopharyngeal carcinoma cases. Moreover, most of the studies that have been conducted using this gene are using tissue rather than cell line as sample. The main aim of the study is to isolate and study the nucleotide sequence of RPL27 gene from human nasopharyngeal carcinoma and normal cell line. The methods used in this study are isolation of total RNA using TRIzol method, reverse transcriptase polymerase chain reaction, and amplification of DNA, purifying, sequencing and finally analyzing the nucleotide sequence of the RPL27 gene using bioinformatics. From this study, it can be found that, there is no nucleotide variation occurs due to mutation in the region of study in RPL27 gene derived from both nasopharyngeal normal and carcinoma cell line. Universiti Malaysia Sarawak, UNIMAS 2012 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/6200/1/BUWANESWARI%20ft.pdf Buwaneswari, Nagayan (2012) Isolation and nucleotide sequence survey of the ribosomal protein gene (RPL27) from human nasopharyngeal-derived cell lines. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | Q Science (General) QH301 Biology Buwaneswari, Nagayan Isolation and nucleotide sequence survey of the ribosomal protein gene (RPL27) from human nasopharyngeal-derived cell lines |
| title | Isolation and nucleotide sequence survey of the ribosomal protein gene (RPL27) from human nasopharyngeal-derived cell lines |
| title_full | Isolation and nucleotide sequence survey of the ribosomal protein gene (RPL27) from human nasopharyngeal-derived cell lines |
| title_fullStr | Isolation and nucleotide sequence survey of the ribosomal protein gene (RPL27) from human nasopharyngeal-derived cell lines |
| title_full_unstemmed | Isolation and nucleotide sequence survey of the ribosomal protein gene (RPL27) from human nasopharyngeal-derived cell lines |
| title_short | Isolation and nucleotide sequence survey of the ribosomal protein gene (RPL27) from human nasopharyngeal-derived cell lines |
| title_sort | isolation and nucleotide sequence survey of the ribosomal protein gene (rpl27) from human nasopharyngeal-derived cell lines |
| topic | Q Science (General) QH301 Biology |
| url | http://ir.unimas.my/id/eprint/6200/ http://ir.unimas.my/id/eprint/6200/1/BUWANESWARI%20ft.pdf |