Isolation and characterization of carbazole-degrading bacteria from mangrove environment
Carbazole is an aromatic heterocyclic organic compound usually found in river sediment that can possess to toxic activities that will lend serious environmental problems. This study was conducted to isolate and characterize carbazole-degrading bacteria from mangrove environment. The samples were c...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2012
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/6198/ http://ir.unimas.my/id/eprint/6198/4/Kho%20full.pdf |
| _version_ | 1848835860168966144 |
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| author | Kho, Tze Chiat |
| author_facet | Kho, Tze Chiat |
| author_sort | Kho, Tze Chiat |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Carbazole is an aromatic heterocyclic organic compound usually found in river sediment that can possess to
toxic activities that will lend serious environmental problems. This study was conducted to isolate and
characterize carbazole-degrading bacteria from mangrove environment. The samples were collected from Bako
National Park and the presence of carbazole-degrading bacteria was confirmed through enrichment culture of the
samples. Screening of the bacteria was successfully done by observing the growth of the strains on ONR7a agar
containing carbazole as sole carbon source. Four different strains of carbazole degrading bacteria that are able to
grow on carbazole source were isolated. Isolate strains were characterized using phenotypic and molecular
techniques. From the BLAST search, four different strains of carbazole degrading bacteria were identified as
Sphingomonas sp., Flavobacterium sp., Gaetbulibacter sp., and Thalassospira sp. with maximum identity of
100%, 97%, 99%, and 99% respectively. All strains appeared as a non-motile Gram-negative rod shaped
bacterium |
| first_indexed | 2025-11-15T06:14:35Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-6198 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:14:35Z |
| publishDate | 2012 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-61982023-08-11T03:23:08Z http://ir.unimas.my/id/eprint/6198/ Isolation and characterization of carbazole-degrading bacteria from mangrove environment Kho, Tze Chiat GE Environmental Sciences QK Botany S Agriculture (General) Carbazole is an aromatic heterocyclic organic compound usually found in river sediment that can possess to toxic activities that will lend serious environmental problems. This study was conducted to isolate and characterize carbazole-degrading bacteria from mangrove environment. The samples were collected from Bako National Park and the presence of carbazole-degrading bacteria was confirmed through enrichment culture of the samples. Screening of the bacteria was successfully done by observing the growth of the strains on ONR7a agar containing carbazole as sole carbon source. Four different strains of carbazole degrading bacteria that are able to grow on carbazole source were isolated. Isolate strains were characterized using phenotypic and molecular techniques. From the BLAST search, four different strains of carbazole degrading bacteria were identified as Sphingomonas sp., Flavobacterium sp., Gaetbulibacter sp., and Thalassospira sp. with maximum identity of 100%, 97%, 99%, and 99% respectively. All strains appeared as a non-motile Gram-negative rod shaped bacterium Universiti Malaysia Sarawak, (UNIMAS) 2012 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/6198/4/Kho%20full.pdf Kho, Tze Chiat (2012) Isolation and characterization of carbazole-degrading bacteria from mangrove environment. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | GE Environmental Sciences QK Botany S Agriculture (General) Kho, Tze Chiat Isolation and characterization of carbazole-degrading bacteria from mangrove environment |
| title | Isolation and characterization of carbazole-degrading bacteria from mangrove environment |
| title_full | Isolation and characterization of carbazole-degrading bacteria from mangrove environment |
| title_fullStr | Isolation and characterization of carbazole-degrading bacteria from mangrove environment |
| title_full_unstemmed | Isolation and characterization of carbazole-degrading bacteria from mangrove environment |
| title_short | Isolation and characterization of carbazole-degrading bacteria from mangrove environment |
| title_sort | isolation and characterization of carbazole-degrading bacteria from mangrove environment |
| topic | GE Environmental Sciences QK Botany S Agriculture (General) |
| url | http://ir.unimas.my/id/eprint/6198/ http://ir.unimas.my/id/eprint/6198/4/Kho%20full.pdf |