Isolation and characterization of carbazole-degrading bacteria from mangrove environment
Carbazole is an aromatic heterocyclic organic compound usually found in river sediment that can possess to toxic activities that will lend serious environmental problems. This study was conducted to isolate and characterize carbazole-degrading bacteria from mangrove environment. The samples were c...
| Main Author: | |
|---|---|
| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2012
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/6198/ http://ir.unimas.my/id/eprint/6198/4/Kho%20full.pdf |
| Summary: | Carbazole is an aromatic heterocyclic organic compound usually found in river sediment that can possess to
toxic activities that will lend serious environmental problems. This study was conducted to isolate and
characterize carbazole-degrading bacteria from mangrove environment. The samples were collected from Bako
National Park and the presence of carbazole-degrading bacteria was confirmed through enrichment culture of the
samples. Screening of the bacteria was successfully done by observing the growth of the strains on ONR7a agar
containing carbazole as sole carbon source. Four different strains of carbazole degrading bacteria that are able to
grow on carbazole source were isolated. Isolate strains were characterized using phenotypic and molecular
techniques. From the BLAST search, four different strains of carbazole degrading bacteria were identified as
Sphingomonas sp., Flavobacterium sp., Gaetbulibacter sp., and Thalassospira sp. with maximum identity of
100%, 97%, 99%, and 99% respectively. All strains appeared as a non-motile Gram-negative rod shaped
bacterium |
|---|