Genome walking of chitinase gene in Sago Palm
Metroxylon sagu which is known as "balau" is a type of plant where its trunk has highly content of starch. The discovery of Metroxylon sagu chitinase gene (MsChi) is important in plant defense response against fungal pathogen. It is a type of protein that catalyzes the hydrolysis of the β...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, (UNIMAS)
2012
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| Online Access: | http://ir.unimas.my/id/eprint/6189/ http://ir.unimas.my/id/eprint/6189/8/SAIFUL%20SHAKIRIN.pdf |
| _version_ | 1848835857633509376 |
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| author | Saiful Shakirin, Bin Rosli |
| author_facet | Saiful Shakirin, Bin Rosli |
| author_sort | Saiful Shakirin, Bin Rosli |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Metroxylon sagu which is known as "balau" is a type of plant where its trunk has highly content of starch. The discovery of Metroxylon sagu chitinase gene (MsChi) is important in plant defense response against fungal pathogen. It is a type of protein that catalyzes the
hydrolysis of the β-1, 4- linkages of N-acetyl-D-glucosamine polymer of chitin which is a major component of the exoskeleton of insects. In order for some gene to be expressed, regulatory element is required in regulating their expression. This project was aimed to
determine the regulatory elements for chitinase gene expression via genomic walking. CTAB method which has been modified is applied for the extraction of total genomic DNA of the sago palm. Via primer-based genomic walking method, the upstream region of chitinase gene in sago palm will be isolated and send for sequencing. However, since no band can be obtained after PCR re-amplification, DNA sequencing step cannot be proceed. |
| first_indexed | 2025-11-15T06:14:32Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-6189 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:14:32Z |
| publishDate | 2012 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-61892024-02-08T02:57:43Z http://ir.unimas.my/id/eprint/6189/ Genome walking of chitinase gene in Sago Palm Saiful Shakirin, Bin Rosli Q Science (General) S Agriculture (General) SB Plant culture Metroxylon sagu which is known as "balau" is a type of plant where its trunk has highly content of starch. The discovery of Metroxylon sagu chitinase gene (MsChi) is important in plant defense response against fungal pathogen. It is a type of protein that catalyzes the hydrolysis of the β-1, 4- linkages of N-acetyl-D-glucosamine polymer of chitin which is a major component of the exoskeleton of insects. In order for some gene to be expressed, regulatory element is required in regulating their expression. This project was aimed to determine the regulatory elements for chitinase gene expression via genomic walking. CTAB method which has been modified is applied for the extraction of total genomic DNA of the sago palm. Via primer-based genomic walking method, the upstream region of chitinase gene in sago palm will be isolated and send for sequencing. However, since no band can be obtained after PCR re-amplification, DNA sequencing step cannot be proceed. Universiti Malaysia Sarawak, (UNIMAS) 2012 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/6189/8/SAIFUL%20SHAKIRIN.pdf Saiful Shakirin, Bin Rosli (2012) Genome walking of chitinase gene in Sago Palm. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | Q Science (General) S Agriculture (General) SB Plant culture Saiful Shakirin, Bin Rosli Genome walking of chitinase gene in Sago Palm |
| title | Genome walking of chitinase gene in Sago Palm |
| title_full | Genome walking of chitinase gene in Sago Palm |
| title_fullStr | Genome walking of chitinase gene in Sago Palm |
| title_full_unstemmed | Genome walking of chitinase gene in Sago Palm |
| title_short | Genome walking of chitinase gene in Sago Palm |
| title_sort | genome walking of chitinase gene in sago palm |
| topic | Q Science (General) S Agriculture (General) SB Plant culture |
| url | http://ir.unimas.my/id/eprint/6189/ http://ir.unimas.my/id/eprint/6189/8/SAIFUL%20SHAKIRIN.pdf |