Genetic diversity of escherichia coli isolated from birds and bats by using pulsed field gel electrophoresis (PFGE)
Escherichia coli (E. coli) belongs to the family of Enterobacteriaceae. E. coli can be found in animals such as birds and bats which cause zoonoses. In this study, a total of 22 isolates of E. coli were used which 11 isolates from birds in Kuching; 6 isolates from broiler chicken and 5 isolates fr...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English English |
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Universiti Malaysia Sarawak, (UNIMAS)
2012
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/6187/ http://ir.unimas.my/id/eprint/6187/1/Larmina.pdf http://ir.unimas.my/id/eprint/6187/4/Larmina%20full.pdf |
| _version_ | 1848835856999120896 |
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| author | Larmina, anak Brown |
| author_facet | Larmina, anak Brown |
| author_sort | Larmina, anak Brown |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Escherichia coli (E. coli) belongs to the family of Enterobacteriaceae. E. coli can be found in animals such as
birds and bats which cause zoonoses. In this study, a total of 22 isolates of E. coli were used which 11
isolates from birds in Kuching; 6 isolates from broiler chicken and 5 isolates from soil while another 11
isolates were bats from different habitats; 2 isolates from Bukit Aup Sibu, 3 isolates from Nanga Merit Kapit,
4 isolates from Kubah National Park and 2 isolates from Matang Wildlife Centre. The E. coli isolates
digested with XbaI generated 6 to 13 bands with sizes of approximately 54.7-668.9 kb. The PFGE profiles
were further analysed by using the RAPDistance program and a dendrogram was constructed based on the
difference of genetic distance. In this study, the genetic diversity of E. coli isolated from birds and bats was
determined from the analysis. Among these samples, the isolates from broiler chicken and soil were more
diverse compared to isolates of bats. |
| first_indexed | 2025-11-15T06:14:31Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-6187 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T06:14:31Z |
| publishDate | 2012 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-61872024-03-06T07:24:53Z http://ir.unimas.my/id/eprint/6187/ Genetic diversity of escherichia coli isolated from birds and bats by using pulsed field gel electrophoresis (PFGE) Larmina, anak Brown GE Environmental Sciences QH426 Genetics QL Zoology Escherichia coli (E. coli) belongs to the family of Enterobacteriaceae. E. coli can be found in animals such as birds and bats which cause zoonoses. In this study, a total of 22 isolates of E. coli were used which 11 isolates from birds in Kuching; 6 isolates from broiler chicken and 5 isolates from soil while another 11 isolates were bats from different habitats; 2 isolates from Bukit Aup Sibu, 3 isolates from Nanga Merit Kapit, 4 isolates from Kubah National Park and 2 isolates from Matang Wildlife Centre. The E. coli isolates digested with XbaI generated 6 to 13 bands with sizes of approximately 54.7-668.9 kb. The PFGE profiles were further analysed by using the RAPDistance program and a dendrogram was constructed based on the difference of genetic distance. In this study, the genetic diversity of E. coli isolated from birds and bats was determined from the analysis. Among these samples, the isolates from broiler chicken and soil were more diverse compared to isolates of bats. Universiti Malaysia Sarawak, (UNIMAS) 2012 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/6187/1/Larmina.pdf text en http://ir.unimas.my/id/eprint/6187/4/Larmina%20full.pdf Larmina, anak Brown (2012) Genetic diversity of escherichia coli isolated from birds and bats by using pulsed field gel electrophoresis (PFGE). [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | GE Environmental Sciences QH426 Genetics QL Zoology Larmina, anak Brown Genetic diversity of escherichia coli isolated from birds and bats by using pulsed field gel electrophoresis (PFGE) |
| title | Genetic diversity of escherichia coli isolated from birds and bats by using pulsed field gel electrophoresis (PFGE) |
| title_full | Genetic diversity of escherichia coli isolated from birds and bats by using pulsed field gel electrophoresis (PFGE) |
| title_fullStr | Genetic diversity of escherichia coli isolated from birds and bats by using pulsed field gel electrophoresis (PFGE) |
| title_full_unstemmed | Genetic diversity of escherichia coli isolated from birds and bats by using pulsed field gel electrophoresis (PFGE) |
| title_short | Genetic diversity of escherichia coli isolated from birds and bats by using pulsed field gel electrophoresis (PFGE) |
| title_sort | genetic diversity of escherichia coli isolated from birds and bats by using pulsed field gel electrophoresis (pfge) |
| topic | GE Environmental Sciences QH426 Genetics QL Zoology |
| url | http://ir.unimas.my/id/eprint/6187/ http://ir.unimas.my/id/eprint/6187/1/Larmina.pdf http://ir.unimas.my/id/eprint/6187/4/Larmina%20full.pdf |