Extracting high-quality DNA and PCR amplification from anuran skin (Bornean toads)
The modified DNA extraction protocol of anuran skin was determined by using mitochondrial DNA from cytochrome oxidase I (COI) gene. There were nine species of 44 individuals comprising two family of anurans; family Bufonidae that comprised genera Phrynoidis, Pesdostibes, Ingerophrynus, Dutthaphrynus...
| Main Author: | |
|---|---|
| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2012
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/6185/ http://ir.unimas.my/id/eprint/6185/4/Sharizzaty%28fulltext%29.pdf |
| _version_ | 1848835856361586688 |
|---|---|
| author | Sharizzaty, binti Mohd Rais |
| author_facet | Sharizzaty, binti Mohd Rais |
| author_sort | Sharizzaty, binti Mohd Rais |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | The modified DNA extraction protocol of anuran skin was determined by using mitochondrial DNA from cytochrome oxidase I (COI) gene. There were nine species of 44 individuals comprising two family of anurans; family Bufonidae that comprised genera Phrynoidis, Pesdostibes, Ingerophrynus, Dutthaphrynus and Ansonia and family Ranidae were included in this study. Specimen were collected from eight localities in Sarawak including Universiti Malaysia Sarawak Campus (UNIMAS),Kubah National Park, Gading National Park, Bako National Park, Mount Santubong, Mount Jagoi, Annah Rais (Padawan) and Batang Ai National Park. In this study, cytochrome oxidase 1 (COI) of mitochondrial gene was used to investigate suitable parameters in amplifying PCR products for skin sampled. The annealing temperature for these selected species ranges from 50 - 63 based on the optimization. From this study, concluded that the modified DNA CTAB extraction protocol was useful to extracting DNA from anuran skin. |
| first_indexed | 2025-11-15T06:14:31Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-6185 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:14:31Z |
| publishDate | 2012 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-61852024-02-08T02:54:42Z http://ir.unimas.my/id/eprint/6185/ Extracting high-quality DNA and PCR amplification from anuran skin (Bornean toads) Sharizzaty, binti Mohd Rais Q Science (General) QL Zoology SF Animal culture The modified DNA extraction protocol of anuran skin was determined by using mitochondrial DNA from cytochrome oxidase I (COI) gene. There were nine species of 44 individuals comprising two family of anurans; family Bufonidae that comprised genera Phrynoidis, Pesdostibes, Ingerophrynus, Dutthaphrynus and Ansonia and family Ranidae were included in this study. Specimen were collected from eight localities in Sarawak including Universiti Malaysia Sarawak Campus (UNIMAS),Kubah National Park, Gading National Park, Bako National Park, Mount Santubong, Mount Jagoi, Annah Rais (Padawan) and Batang Ai National Park. In this study, cytochrome oxidase 1 (COI) of mitochondrial gene was used to investigate suitable parameters in amplifying PCR products for skin sampled. The annealing temperature for these selected species ranges from 50 - 63 based on the optimization. From this study, concluded that the modified DNA CTAB extraction protocol was useful to extracting DNA from anuran skin. Universiti Malaysia Sarawak, (UNIMAS) 2012 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/6185/4/Sharizzaty%28fulltext%29.pdf Sharizzaty, binti Mohd Rais (2012) Extracting high-quality DNA and PCR amplification from anuran skin (Bornean toads). [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | Q Science (General) QL Zoology SF Animal culture Sharizzaty, binti Mohd Rais Extracting high-quality DNA and PCR amplification from anuran skin (Bornean toads) |
| title | Extracting high-quality DNA and PCR amplification from anuran skin (Bornean toads) |
| title_full | Extracting high-quality DNA and PCR amplification from anuran skin (Bornean toads) |
| title_fullStr | Extracting high-quality DNA and PCR amplification from anuran skin (Bornean toads) |
| title_full_unstemmed | Extracting high-quality DNA and PCR amplification from anuran skin (Bornean toads) |
| title_short | Extracting high-quality DNA and PCR amplification from anuran skin (Bornean toads) |
| title_sort | extracting high-quality dna and pcr amplification from anuran skin (bornean toads) |
| topic | Q Science (General) QL Zoology SF Animal culture |
| url | http://ir.unimas.my/id/eprint/6185/ http://ir.unimas.my/id/eprint/6185/4/Sharizzaty%28fulltext%29.pdf |