Establishing the protocol of molecular study of butterfly (lepidoptera: nymphalidae)
Optimization of polymerase chain reaction (PCR) using two kinds of primers of gene cytochrome oxidase subunit I (COI), which are the universal primer and COI_2; was done for establishing the protocol of molecular study of Nymphalidae. Five parts of the nymphalids were extracted to determine which yi...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, (UNIMAS)
2012
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| Online Access: | http://ir.unimas.my/id/eprint/6182/ http://ir.unimas.my/id/eprint/6182/8/ESTABLISHING%20THE%20PROTOCOL%20OF%20MOLECULAR%20STUDY%20OF%20BUTTERFLY%20%28OCR%29.pdf |
| _version_ | 1848835855360196608 |
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| author | Nur Alwanie, Binti Maruji |
| author_facet | Nur Alwanie, Binti Maruji |
| author_sort | Nur Alwanie, Binti Maruji |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Optimization of polymerase chain reaction (PCR) using two kinds of primers of gene cytochrome oxidase subunit I (COI), which are the universal primer and COI_2; was done for establishing the protocol of molecular study of Nymphalidae. Five parts of the nymphalids were extracted to determine which yield the DNA product. These parts were the head, thorax, abdomen, legs and antennae, and the extraction was done by using the CTAB method. The extraction for the part head, thorax and abdomen were successful. Dried and fresh specimens were also used in this study and both yield DNA extraction product. The parameters for the optimization were designed into three programmes. PCR gradient was run 15 times and only one sample sent for sequencing. The amplification of mtDNA of universal primer COI and COI_2 were tested to obtain the
PCR products, of which the amplification of DNA using COI_2 shows a positive result. Percentage of the successful PCR was calculated and of the positive result, the percentages for both good and poor bands were also calculated to interpret the successfulness of the PCR optimization. |
| first_indexed | 2025-11-15T06:14:30Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-6182 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:14:30Z |
| publishDate | 2012 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-61822023-11-15T06:31:50Z http://ir.unimas.my/id/eprint/6182/ Establishing the protocol of molecular study of butterfly (lepidoptera: nymphalidae) Nur Alwanie, Binti Maruji Q Science (General) QL Zoology Optimization of polymerase chain reaction (PCR) using two kinds of primers of gene cytochrome oxidase subunit I (COI), which are the universal primer and COI_2; was done for establishing the protocol of molecular study of Nymphalidae. Five parts of the nymphalids were extracted to determine which yield the DNA product. These parts were the head, thorax, abdomen, legs and antennae, and the extraction was done by using the CTAB method. The extraction for the part head, thorax and abdomen were successful. Dried and fresh specimens were also used in this study and both yield DNA extraction product. The parameters for the optimization were designed into three programmes. PCR gradient was run 15 times and only one sample sent for sequencing. The amplification of mtDNA of universal primer COI and COI_2 were tested to obtain the PCR products, of which the amplification of DNA using COI_2 shows a positive result. Percentage of the successful PCR was calculated and of the positive result, the percentages for both good and poor bands were also calculated to interpret the successfulness of the PCR optimization. Universiti Malaysia Sarawak, (UNIMAS) 2012 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/6182/8/ESTABLISHING%20THE%20PROTOCOL%20OF%20MOLECULAR%20STUDY%20OF%20BUTTERFLY%20%28OCR%29.pdf Nur Alwanie, Binti Maruji (2012) Establishing the protocol of molecular study of butterfly (lepidoptera: nymphalidae). [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | Q Science (General) QL Zoology Nur Alwanie, Binti Maruji Establishing the protocol of molecular study of butterfly (lepidoptera: nymphalidae) |
| title | Establishing the protocol of molecular study of butterfly (lepidoptera: nymphalidae) |
| title_full | Establishing the protocol of molecular study of butterfly (lepidoptera: nymphalidae) |
| title_fullStr | Establishing the protocol of molecular study of butterfly (lepidoptera: nymphalidae) |
| title_full_unstemmed | Establishing the protocol of molecular study of butterfly (lepidoptera: nymphalidae) |
| title_short | Establishing the protocol of molecular study of butterfly (lepidoptera: nymphalidae) |
| title_sort | establishing the protocol of molecular study of butterfly (lepidoptera: nymphalidae) |
| topic | Q Science (General) QL Zoology |
| url | http://ir.unimas.my/id/eprint/6182/ http://ir.unimas.my/id/eprint/6182/8/ESTABLISHING%20THE%20PROTOCOL%20OF%20MOLECULAR%20STUDY%20OF%20BUTTERFLY%20%28OCR%29.pdf |