DNA genotyping of Duabanga moluccana (Sawih) using ISSR Marker
The genetic diversity of Duabanga moluccana in 3 populations was characterized using inter-simple sequence repeats (ISSR) technique. Two ISSR primers: CAG(CA)8 and (ACC)6G were screened, of which both are polymorphic and provide informative patterns to determine genetic relationships. ISSR amplifi...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2012
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| Online Access: | http://ir.unimas.my/id/eprint/6174/ http://ir.unimas.my/id/eprint/6174/8/DNA%20Genotyping%20of%20Duabanga%20moluccana%20%28sawih%29%20using%20ISSR%20Markers%28OCR%29.pdf |
| Summary: | The genetic diversity of Duabanga moluccana in 3 populations was characterized using inter-simple sequence
repeats (ISSR) technique. Two ISSR primers: CAG(CA)8 and (ACC)6G were screened, of which both are
polymorphic and provide informative patterns to determine genetic relationships. ISSR amplification was conducted
on 90 samples from 3 populations, and all 40 loci detected were polymorphic. The percentage of PPL at Mukah,
Niah and Tatau were 82.5%, 90.00% and 62.50% respectively, with an average of 78.33%. Nei’s gene diversity (h)
and Shannon’s Information Index (I) of D. moluccana at the species level were 0.2206 and 0.3569, respectively. The
genetic differentiation coefficient (Gst) among populations was 0.1234. The gene flow (Nm) among populations was
1.7759, indicating that gene flow was high among populations of D. moluccana. |
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