Developing an effective microbial formulation for biodegradation of pineapple waste vis solid state fermentation

This project aims to formulate an effective microbial formulation for biodegradation of pineapple waste. A strain of fungus, Trichoderma harzianum UMAS SD17 and two strains of Bacillussp., Bacillus licheniformis UMAS P7 and Bacillus amyloliquefaciens UMAS 1002 were used with varying co-cultivation...

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Main Author: Siti Aisyah, Binti Mohd Zaman
Format: Final Year Project Report / IMRAD
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2012
Subjects:
Online Access:http://ir.unimas.my/id/eprint/6172/
http://ir.unimas.my/id/eprint/6172/8/Siti%20Aisyah.pdf
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author Siti Aisyah, Binti Mohd Zaman
author_facet Siti Aisyah, Binti Mohd Zaman
author_sort Siti Aisyah, Binti Mohd Zaman
building UNIMAS Institutional Repository
collection Online Access
description This project aims to formulate an effective microbial formulation for biodegradation of pineapple waste. A strain of fungus, Trichoderma harzianum UMAS SD17 and two strains of Bacillussp., Bacillus licheniformis UMAS P7 and Bacillus amyloliquefaciens UMAS 1002 were used with varying co-cultivation in solid state fermentation where the pineapple waste are used as the substrate. Eight analytical analysis were done to determine the most efficient microbial formulation, which includes, protein content, carbon to nitrogen ratio, biomass dry weight, glucose and reducing sugar concentration and the level of cellulose, hemicellulose and lignin degradation. Based on the data obtained, the fermentation with co-cultivation of all three microbes shows the highest cellulose degradation from 37.65% to 12.93%, and the highest hemicelluloses degradation from 6.87% to 2.36%. It also shows the second highest rate of lignin degradation after formulation of B.licheniformis and T.harzianum, from 14.5% to 9.5%. After 24 days of fermentation, this formulation also shows the closest C:N ratio to the ideal compost ratio of 25:1 at 50.12:1, thus suggesting that this formulation of microbes consisting of T.harzianum, B.licheniformis and B.amyloliquefaciens is the most efficient formulation for biodegradation of pineapple waste and can be further used for a complete composting process.
first_indexed 2025-11-15T06:14:29Z
format Final Year Project Report / IMRAD
id unimas-6172
institution Universiti Malaysia Sarawak
institution_category Local University
language English
last_indexed 2025-11-15T06:14:29Z
publishDate 2012
publisher Universiti Malaysia Sarawak, (UNIMAS)
recordtype eprints
repository_type Digital Repository
spelling unimas-61722024-02-08T02:51:34Z http://ir.unimas.my/id/eprint/6172/ Developing an effective microbial formulation for biodegradation of pineapple waste vis solid state fermentation Siti Aisyah, Binti Mohd Zaman GE Environmental Sciences Q Science (General) This project aims to formulate an effective microbial formulation for biodegradation of pineapple waste. A strain of fungus, Trichoderma harzianum UMAS SD17 and two strains of Bacillussp., Bacillus licheniformis UMAS P7 and Bacillus amyloliquefaciens UMAS 1002 were used with varying co-cultivation in solid state fermentation where the pineapple waste are used as the substrate. Eight analytical analysis were done to determine the most efficient microbial formulation, which includes, protein content, carbon to nitrogen ratio, biomass dry weight, glucose and reducing sugar concentration and the level of cellulose, hemicellulose and lignin degradation. Based on the data obtained, the fermentation with co-cultivation of all three microbes shows the highest cellulose degradation from 37.65% to 12.93%, and the highest hemicelluloses degradation from 6.87% to 2.36%. It also shows the second highest rate of lignin degradation after formulation of B.licheniformis and T.harzianum, from 14.5% to 9.5%. After 24 days of fermentation, this formulation also shows the closest C:N ratio to the ideal compost ratio of 25:1 at 50.12:1, thus suggesting that this formulation of microbes consisting of T.harzianum, B.licheniformis and B.amyloliquefaciens is the most efficient formulation for biodegradation of pineapple waste and can be further used for a complete composting process. Universiti Malaysia Sarawak, (UNIMAS) 2012 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/6172/8/Siti%20Aisyah.pdf Siti Aisyah, Binti Mohd Zaman (2012) Developing an effective microbial formulation for biodegradation of pineapple waste vis solid state fermentation. [Final Year Project Report / IMRAD] (Unpublished)
spellingShingle GE Environmental Sciences
Q Science (General)
Siti Aisyah, Binti Mohd Zaman
Developing an effective microbial formulation for biodegradation of pineapple waste vis solid state fermentation
title Developing an effective microbial formulation for biodegradation of pineapple waste vis solid state fermentation
title_full Developing an effective microbial formulation for biodegradation of pineapple waste vis solid state fermentation
title_fullStr Developing an effective microbial formulation for biodegradation of pineapple waste vis solid state fermentation
title_full_unstemmed Developing an effective microbial formulation for biodegradation of pineapple waste vis solid state fermentation
title_short Developing an effective microbial formulation for biodegradation of pineapple waste vis solid state fermentation
title_sort developing an effective microbial formulation for biodegradation of pineapple waste vis solid state fermentation
topic GE Environmental Sciences
Q Science (General)
url http://ir.unimas.my/id/eprint/6172/
http://ir.unimas.my/id/eprint/6172/8/Siti%20Aisyah.pdf