Detection of oprl gene and antibiotic resistance of pseudomonas aeruginosa from aquaculture enviroment
Pseudomonas aeruginosa is a gram-negative rod shape bacterium belonging to family Pseudomonadaceae. The species is a highly adaptable opportunistic pathogen capable of surviving in a variety of environment, including aquaculture environment. Antibiotics are used in the aquaculture environment, and...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English English |
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Universiti Malaysia Sarawak, (UNIMAS)
2012
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/6168/ http://ir.unimas.my/id/eprint/6168/1/Spencer%20%2824pgs%29.pdf http://ir.unimas.my/id/eprint/6168/4/Spencer%20%28fulltext%29.pdf |
| _version_ | 1848835853187547136 |
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| author | Spencer Carlos, anak Bennet |
| author_facet | Spencer Carlos, anak Bennet |
| author_sort | Spencer Carlos, anak Bennet |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Pseudomonas aeruginosa is a gram-negative rod shape bacterium belonging to family Pseudomonadaceae. The species is a highly adaptable opportunistic pathogen capable of surviving in a variety of environment, including aquaculture environment. Antibiotics are
used in the aquaculture environment, and their usage poses a risk of potential transfer of resistance to human and animal pathogens. This study was conducted to isolate P. aeruginosa and identify them based on the PCR detection of oprL gene locus. These species were isolated from fish, prawn and water. Antibiotic resistance pattern was determined by conducting antibiotic susceptibility tests on the isolates. Thirteen isolates of P. aeruginosa were isolated and all of the isolates show resistance to at least one antibiotic. Highest level of resistance was observed against ampicillin and erythromycin and the
lowest group was against gentamicin, norflaxin and nalidixic acid. |
| first_indexed | 2025-11-15T06:14:28Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-6168 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T06:14:28Z |
| publishDate | 2012 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-61682024-02-08T02:49:33Z http://ir.unimas.my/id/eprint/6168/ Detection of oprl gene and antibiotic resistance of pseudomonas aeruginosa from aquaculture enviroment Spencer Carlos, anak Bennet GE Environmental Sciences SH Aquaculture. Fisheries. Angling Pseudomonas aeruginosa is a gram-negative rod shape bacterium belonging to family Pseudomonadaceae. The species is a highly adaptable opportunistic pathogen capable of surviving in a variety of environment, including aquaculture environment. Antibiotics are used in the aquaculture environment, and their usage poses a risk of potential transfer of resistance to human and animal pathogens. This study was conducted to isolate P. aeruginosa and identify them based on the PCR detection of oprL gene locus. These species were isolated from fish, prawn and water. Antibiotic resistance pattern was determined by conducting antibiotic susceptibility tests on the isolates. Thirteen isolates of P. aeruginosa were isolated and all of the isolates show resistance to at least one antibiotic. Highest level of resistance was observed against ampicillin and erythromycin and the lowest group was against gentamicin, norflaxin and nalidixic acid. Universiti Malaysia Sarawak, (UNIMAS) 2012 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/6168/1/Spencer%20%2824pgs%29.pdf text en http://ir.unimas.my/id/eprint/6168/4/Spencer%20%28fulltext%29.pdf Spencer Carlos, anak Bennet (2012) Detection of oprl gene and antibiotic resistance of pseudomonas aeruginosa from aquaculture enviroment. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | GE Environmental Sciences SH Aquaculture. Fisheries. Angling Spencer Carlos, anak Bennet Detection of oprl gene and antibiotic resistance of pseudomonas aeruginosa from aquaculture enviroment |
| title | Detection of oprl gene and antibiotic resistance of pseudomonas aeruginosa from aquaculture enviroment |
| title_full | Detection of oprl gene and antibiotic resistance of pseudomonas aeruginosa from aquaculture enviroment |
| title_fullStr | Detection of oprl gene and antibiotic resistance of pseudomonas aeruginosa from aquaculture enviroment |
| title_full_unstemmed | Detection of oprl gene and antibiotic resistance of pseudomonas aeruginosa from aquaculture enviroment |
| title_short | Detection of oprl gene and antibiotic resistance of pseudomonas aeruginosa from aquaculture enviroment |
| title_sort | detection of oprl gene and antibiotic resistance of pseudomonas aeruginosa from aquaculture enviroment |
| topic | GE Environmental Sciences SH Aquaculture. Fisheries. Angling |
| url | http://ir.unimas.my/id/eprint/6168/ http://ir.unimas.my/id/eprint/6168/1/Spencer%20%2824pgs%29.pdf http://ir.unimas.my/id/eprint/6168/4/Spencer%20%28fulltext%29.pdf |