Assessment of microbiological activities of empurau fish (Tor Tambraides) of local fish farm

Enterobacteriaceae is a member of gram-negative bacilli and contain more than 30 genera. They act as the main indicator for the bacteriological water quality. Study was conducted to determine the occurrence of Enterobacteriaceae family in Empurau (Tor Tambraides) pond at Indigenous Fish Research...

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Main Author: Famila Farvin, Binti Ghulam Dasthagir
Format: Final Year Project Report / IMRAD
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2012
Subjects:
Online Access:http://ir.unimas.my/id/eprint/6045/
http://ir.unimas.my/id/eprint/6045/1/Famila%20Farvin%20ft.pdf
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author Famila Farvin, Binti Ghulam Dasthagir
author_facet Famila Farvin, Binti Ghulam Dasthagir
author_sort Famila Farvin, Binti Ghulam Dasthagir
building UNIMAS Institutional Repository
collection Online Access
description Enterobacteriaceae is a member of gram-negative bacilli and contain more than 30 genera. They act as the main indicator for the bacteriological water quality. Study was conducted to determine the occurrence of Enterobacteriaceae family in Empurau (Tor Tambraides) pond at Indigenous Fish Research & Production Centre (IFRPC) in Tarat, Sarawak in which this it could reflect the level of microbe found in the pond. Studies also been conducted for detection on any possible pathogenic bacteria that might cause infectious disease in fish pond. Besides that, studies were conducted for determining the relationship between physicochemical parameter and microbiological parameter with the development of the fish in the pond. Water sample from the pond were collected and analyzed for the presence of Enterobacteriaceae. Sample plated on Nutrient Agar and colonies formed were tested on gram staining. Colonies with Gram-negative were further analyzed with (GTG)5 PCR. The phylogenetic tree was constructed based on the DNA fingerprinting to illustrate the relationship among the bacteria isolates. 11 randomly picked samples were tested with API 20E kit for identification of the bacteria.
first_indexed 2025-11-15T06:13:55Z
format Final Year Project Report / IMRAD
id unimas-6045
institution Universiti Malaysia Sarawak
institution_category Local University
language English
last_indexed 2025-11-15T06:13:55Z
publishDate 2012
publisher Universiti Malaysia Sarawak, (UNIMAS)
recordtype eprints
repository_type Digital Repository
spelling unimas-60452023-10-25T08:29:32Z http://ir.unimas.my/id/eprint/6045/ Assessment of microbiological activities of empurau fish (Tor Tambraides) of local fish farm Famila Farvin, Binti Ghulam Dasthagir QE Geology SH Aquaculture. Fisheries. Angling Enterobacteriaceae is a member of gram-negative bacilli and contain more than 30 genera. They act as the main indicator for the bacteriological water quality. Study was conducted to determine the occurrence of Enterobacteriaceae family in Empurau (Tor Tambraides) pond at Indigenous Fish Research & Production Centre (IFRPC) in Tarat, Sarawak in which this it could reflect the level of microbe found in the pond. Studies also been conducted for detection on any possible pathogenic bacteria that might cause infectious disease in fish pond. Besides that, studies were conducted for determining the relationship between physicochemical parameter and microbiological parameter with the development of the fish in the pond. Water sample from the pond were collected and analyzed for the presence of Enterobacteriaceae. Sample plated on Nutrient Agar and colonies formed were tested on gram staining. Colonies with Gram-negative were further analyzed with (GTG)5 PCR. The phylogenetic tree was constructed based on the DNA fingerprinting to illustrate the relationship among the bacteria isolates. 11 randomly picked samples were tested with API 20E kit for identification of the bacteria. Universiti Malaysia Sarawak, (UNIMAS) 2012 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/6045/1/Famila%20Farvin%20ft.pdf Famila Farvin, Binti Ghulam Dasthagir (2012) Assessment of microbiological activities of empurau fish (Tor Tambraides) of local fish farm. [Final Year Project Report / IMRAD] (Unpublished)
spellingShingle QE Geology
SH Aquaculture. Fisheries. Angling
Famila Farvin, Binti Ghulam Dasthagir
Assessment of microbiological activities of empurau fish (Tor Tambraides) of local fish farm
title Assessment of microbiological activities of empurau fish (Tor Tambraides) of local fish farm
title_full Assessment of microbiological activities of empurau fish (Tor Tambraides) of local fish farm
title_fullStr Assessment of microbiological activities of empurau fish (Tor Tambraides) of local fish farm
title_full_unstemmed Assessment of microbiological activities of empurau fish (Tor Tambraides) of local fish farm
title_short Assessment of microbiological activities of empurau fish (Tor Tambraides) of local fish farm
title_sort assessment of microbiological activities of empurau fish (tor tambraides) of local fish farm
topic QE Geology
SH Aquaculture. Fisheries. Angling
url http://ir.unimas.my/id/eprint/6045/
http://ir.unimas.my/id/eprint/6045/1/Famila%20Farvin%20ft.pdf