Chemical studies and biological activities of extractives from piper betle leaves
Isolation of compounds and the biological activities of the extracts from the Piper betle leaves were carried out. The sample was extracted by using solvent extraction method using four different solvents with increasing polarity which are hexane, dichloromethane (DCM), ethyl acetate and methanol. F...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English English |
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Universiti Malaysia Sarawak (UNIMAS)
2008
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| Online Access: | http://ir.unimas.my/id/eprint/5640/ http://ir.unimas.my/id/eprint/5640/1/DAYANG%20HALIMATULZAHRAH%20BT.%20ABANG%20KAMALUDDIN%2024pgs.pdf http://ir.unimas.my/id/eprint/5640/4/Dayang%20Halimatulzahrah%20ft.pdf |
| _version_ | 1848835717900271616 |
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| author | Dayang Halimatulzahrah, bt. Abang Kamaluddin |
| author_facet | Dayang Halimatulzahrah, bt. Abang Kamaluddin |
| author_sort | Dayang Halimatulzahrah, bt. Abang Kamaluddin |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Isolation of compounds and the biological activities of the extracts from the Piper betle leaves were carried out. The sample was extracted by using solvent extraction method using four different solvents with increasing polarity which are hexane, dichloromethane (DCM), ethyl acetate and methanol. Further fractionation and isolation by using chromatographic methods afforded a pure compound with molecular mass of 204.2 when subjected to GC-MS analysis, which is the compound amorphene. A semi pure compound was also obtained, giving one sharp peak when subjected to GC-FID analysis. Toxicity test revealed that the extractives of Piper betle leaves are not toxic against brine shrimp, Artemia salina, with LC50 greater than 100ppm. In antitermite toxicity test, DCM crude extract was found to be the most toxic, with 100.0% mortality after three days for three different concentrations. The antibacterial test showed that the DCM extract gave growth inhibition to the bacteria at 10.0% concentration. It was also found that the DCM crude extract is more toxic towards Escherichia coli as compared to Staphylococcus aureus. |
| first_indexed | 2025-11-15T06:12:19Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-5640 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T06:12:19Z |
| publishDate | 2008 |
| publisher | Universiti Malaysia Sarawak (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-56402023-07-05T09:17:13Z http://ir.unimas.my/id/eprint/5640/ Chemical studies and biological activities of extractives from piper betle leaves Dayang Halimatulzahrah, bt. Abang Kamaluddin SB Plant culture Isolation of compounds and the biological activities of the extracts from the Piper betle leaves were carried out. The sample was extracted by using solvent extraction method using four different solvents with increasing polarity which are hexane, dichloromethane (DCM), ethyl acetate and methanol. Further fractionation and isolation by using chromatographic methods afforded a pure compound with molecular mass of 204.2 when subjected to GC-MS analysis, which is the compound amorphene. A semi pure compound was also obtained, giving one sharp peak when subjected to GC-FID analysis. Toxicity test revealed that the extractives of Piper betle leaves are not toxic against brine shrimp, Artemia salina, with LC50 greater than 100ppm. In antitermite toxicity test, DCM crude extract was found to be the most toxic, with 100.0% mortality after three days for three different concentrations. The antibacterial test showed that the DCM extract gave growth inhibition to the bacteria at 10.0% concentration. It was also found that the DCM crude extract is more toxic towards Escherichia coli as compared to Staphylococcus aureus. Universiti Malaysia Sarawak (UNIMAS) 2008 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/5640/1/DAYANG%20HALIMATULZAHRAH%20BT.%20ABANG%20KAMALUDDIN%2024pgs.pdf text en http://ir.unimas.my/id/eprint/5640/4/Dayang%20Halimatulzahrah%20ft.pdf Dayang Halimatulzahrah, bt. Abang Kamaluddin (2008) Chemical studies and biological activities of extractives from piper betle leaves. [Final Year Project Report / IMRAD] |
| spellingShingle | SB Plant culture Dayang Halimatulzahrah, bt. Abang Kamaluddin Chemical studies and biological activities of extractives from piper betle leaves |
| title | Chemical studies and biological activities of extractives from piper betle leaves |
| title_full | Chemical studies and biological activities of extractives from piper betle leaves |
| title_fullStr | Chemical studies and biological activities of extractives from piper betle leaves |
| title_full_unstemmed | Chemical studies and biological activities of extractives from piper betle leaves |
| title_short | Chemical studies and biological activities of extractives from piper betle leaves |
| title_sort | chemical studies and biological activities of extractives from piper betle leaves |
| topic | SB Plant culture |
| url | http://ir.unimas.my/id/eprint/5640/ http://ir.unimas.my/id/eprint/5640/1/DAYANG%20HALIMATULZAHRAH%20BT.%20ABANG%20KAMALUDDIN%2024pgs.pdf http://ir.unimas.my/id/eprint/5640/4/Dayang%20Halimatulzahrah%20ft.pdf |