Molecular cloning and sequence variations of cDNA encoding for trypsin inhibitor from kelampayan (Neolamarckia cadamba)
Proteinase inhibitors are important in plant defense mechanisms. The gene that is responsible for it is Kunitz-type trypsin inhibitor (KTI) which reduces the digestability and nutritional quality of the leaves of the tree which is infected by insect pests or microbes. The objectives of this study a...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, (UNIMAS)
2011
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/5335/ http://ir.unimas.my/id/eprint/5335/3/Melanie%20Ann%20Perera.pdf |
| _version_ | 1848835641333252096 |
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| author | Melanie Ann, Perera |
| author_facet | Melanie Ann, Perera |
| author_sort | Melanie Ann, Perera |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Proteinase inhibitors are important in plant defense mechanisms. The gene that is responsible for it is
Kunitz-type trypsin inhibitor (KTI) which reduces the digestability and nutritional quality of the leaves of the tree which is infected by insect pests or microbes. The objectives of this study are to clone the cDNA
encoding for trypsin inhibitor and to identify single nucleotide polymorphisms (SNPs) through in-silico
analyses. Neolamarckia cadamba (Kelampayan) is used in this study because of its fast growth rate and economic importance. Total RNA was isolated from developing xylem samples and reverse transcribed into cDNA. The ~323 bp gene was then amplified and cloned into pGEM-ⓇT Easy vector systems and sent for automated sequencing. Results showed that a miraculin-like gene was isolated. Further analysis revealed that the gene belongs to the plant Kunitz serine trypsin inhibitor (STI) family of proteinase inhibitors, with the same functions as KTI gene. A total of 27 SNPs were identified with 22 synonymous and 5 non-synonymous. |
| first_indexed | 2025-11-15T06:11:06Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-5335 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:11:06Z |
| publishDate | 2011 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-53352023-08-28T08:55:27Z http://ir.unimas.my/id/eprint/5335/ Molecular cloning and sequence variations of cDNA encoding for trypsin inhibitor from kelampayan (Neolamarckia cadamba) Melanie Ann, Perera Q Science (General) Proteinase inhibitors are important in plant defense mechanisms. The gene that is responsible for it is Kunitz-type trypsin inhibitor (KTI) which reduces the digestability and nutritional quality of the leaves of the tree which is infected by insect pests or microbes. The objectives of this study are to clone the cDNA encoding for trypsin inhibitor and to identify single nucleotide polymorphisms (SNPs) through in-silico analyses. Neolamarckia cadamba (Kelampayan) is used in this study because of its fast growth rate and economic importance. Total RNA was isolated from developing xylem samples and reverse transcribed into cDNA. The ~323 bp gene was then amplified and cloned into pGEM-ⓇT Easy vector systems and sent for automated sequencing. Results showed that a miraculin-like gene was isolated. Further analysis revealed that the gene belongs to the plant Kunitz serine trypsin inhibitor (STI) family of proteinase inhibitors, with the same functions as KTI gene. A total of 27 SNPs were identified with 22 synonymous and 5 non-synonymous. Universiti Malaysia Sarawak, (UNIMAS) 2011 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/5335/3/Melanie%20Ann%20Perera.pdf Melanie Ann, Perera (2011) Molecular cloning and sequence variations of cDNA encoding for trypsin inhibitor from kelampayan (Neolamarckia cadamba). [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | Q Science (General) Melanie Ann, Perera Molecular cloning and sequence variations of cDNA encoding for trypsin inhibitor from kelampayan (Neolamarckia cadamba) |
| title | Molecular cloning and sequence variations of cDNA encoding for trypsin inhibitor from kelampayan (Neolamarckia cadamba) |
| title_full | Molecular cloning and sequence variations of cDNA encoding for trypsin inhibitor from kelampayan (Neolamarckia cadamba) |
| title_fullStr | Molecular cloning and sequence variations of cDNA encoding for trypsin inhibitor from kelampayan (Neolamarckia cadamba) |
| title_full_unstemmed | Molecular cloning and sequence variations of cDNA encoding for trypsin inhibitor from kelampayan (Neolamarckia cadamba) |
| title_short | Molecular cloning and sequence variations of cDNA encoding for trypsin inhibitor from kelampayan (Neolamarckia cadamba) |
| title_sort | molecular cloning and sequence variations of cdna encoding for trypsin inhibitor from kelampayan (neolamarckia cadamba) |
| topic | Q Science (General) |
| url | http://ir.unimas.my/id/eprint/5335/ http://ir.unimas.my/id/eprint/5335/3/Melanie%20Ann%20Perera.pdf |