Molecular cloning and expression study of proline-rich ena/vasp ligand 2a (PREL-2a) gene in zebrafish development
Proline rich Ena/VASP Ligand 2 (PREL 2) is designated as Lamellipodin (Lpd) in mammals. PREL-2 possesses the ability to colocalize with Ena/VASP proteins at the tips of lamellipodia. Mammalian PREL-2 was found to have significant functions on cell signalling, direct migration, promoting axon guidan...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, (UNIMAS)
2011
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| Online Access: | http://ir.unimas.my/id/eprint/5333/ http://ir.unimas.my/id/eprint/5333/1/Winnie%20Chuo%20ft.pdf |
| _version_ | 1848835640779603968 |
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| author | Chuo, Winnie |
| author_facet | Chuo, Winnie |
| author_sort | Chuo, Winnie |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Proline rich Ena/VASP Ligand 2 (PREL 2) is designated as Lamellipodin (Lpd) in mammals. PREL-2 possesses the ability to colocalize with Ena/VASP proteins at the tips
of lamellipodia. Mammalian PREL-2 was found to have significant functions on cell signalling, direct migration, promoting axon guidance, affecting adhesion and regulating lamellipodial dynamics. Zebrafish PREL-2 gene is duplicated into two copies namely, PREL-2a and PREL-2b during a genome duplication event. The expression data of PREL-2 family only available for selected adult organs in human and mice but still no detailed information of PREL-2a in the embryonic stages. In this study, the cloning and expression profile of zebrafish PREL-2a gene was described. Semiquantitative RT-PCR analysis revealed that zebrafish PREL-2a gene is expressed throughout the development. PREL-2a gene expression was first expressed at 1K cells stage (3hpf) and constantly expressed throughout zebrafish development up to 5dpf. The RT-PCR result indicated that PREL-2a
gene show maternal and zygotic expression. |
| first_indexed | 2025-11-15T06:11:05Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-5333 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:11:05Z |
| publishDate | 2011 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-53332023-10-31T02:34:07Z http://ir.unimas.my/id/eprint/5333/ Molecular cloning and expression study of proline-rich ena/vasp ligand 2a (PREL-2a) gene in zebrafish development Chuo, Winnie Q Science (General) Proline rich Ena/VASP Ligand 2 (PREL 2) is designated as Lamellipodin (Lpd) in mammals. PREL-2 possesses the ability to colocalize with Ena/VASP proteins at the tips of lamellipodia. Mammalian PREL-2 was found to have significant functions on cell signalling, direct migration, promoting axon guidance, affecting adhesion and regulating lamellipodial dynamics. Zebrafish PREL-2 gene is duplicated into two copies namely, PREL-2a and PREL-2b during a genome duplication event. The expression data of PREL-2 family only available for selected adult organs in human and mice but still no detailed information of PREL-2a in the embryonic stages. In this study, the cloning and expression profile of zebrafish PREL-2a gene was described. Semiquantitative RT-PCR analysis revealed that zebrafish PREL-2a gene is expressed throughout the development. PREL-2a gene expression was first expressed at 1K cells stage (3hpf) and constantly expressed throughout zebrafish development up to 5dpf. The RT-PCR result indicated that PREL-2a gene show maternal and zygotic expression. Universiti Malaysia Sarawak, (UNIMAS) 2011 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/5333/1/Winnie%20Chuo%20ft.pdf Chuo, Winnie (2011) Molecular cloning and expression study of proline-rich ena/vasp ligand 2a (PREL-2a) gene in zebrafish development. [Final Year Project Report / IMRAD] |
| spellingShingle | Q Science (General) Chuo, Winnie Molecular cloning and expression study of proline-rich ena/vasp ligand 2a (PREL-2a) gene in zebrafish development |
| title | Molecular cloning and expression study of proline-rich ena/vasp ligand 2a (PREL-2a) gene in zebrafish development |
| title_full | Molecular cloning and expression study of proline-rich ena/vasp ligand 2a (PREL-2a) gene in zebrafish development |
| title_fullStr | Molecular cloning and expression study of proline-rich ena/vasp ligand 2a (PREL-2a) gene in zebrafish development |
| title_full_unstemmed | Molecular cloning and expression study of proline-rich ena/vasp ligand 2a (PREL-2a) gene in zebrafish development |
| title_short | Molecular cloning and expression study of proline-rich ena/vasp ligand 2a (PREL-2a) gene in zebrafish development |
| title_sort | molecular cloning and expression study of proline-rich ena/vasp ligand 2a (prel-2a) gene in zebrafish development |
| topic | Q Science (General) |
| url | http://ir.unimas.my/id/eprint/5333/ http://ir.unimas.my/id/eprint/5333/1/Winnie%20Chuo%20ft.pdf |