Molecular cloning and expression study of growth-factor receptor bound protein 7 (grb7) gene in zebrafish development
Grb7 is a multi-domain adaptor protein which has been postulated to play a role in the regulation of cell migration. The ability to regulate cell migration implies that the protein may be an important component in the progression of cancer as well as in the embryonic development of an organism. Ther...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English English |
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Universiti Malaysia Sarawak, (UNIMAS)
2011
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| Online Access: | http://ir.unimas.my/id/eprint/5331/ http://ir.unimas.my/id/eprint/5331/1/Shing%20%2824pgs%29.pdf http://ir.unimas.my/id/eprint/5331/3/Tan%20Shing%20Cheng.pdf |
| _version_ | 1848835640244830208 |
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| author | Tan, Shing Cheng |
| author_facet | Tan, Shing Cheng |
| author_sort | Tan, Shing Cheng |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Grb7 is a multi-domain adaptor protein which has been postulated to play a role in the regulation of cell migration. The ability to regulate cell migration implies that the protein may be an important component in the progression of cancer as well as in the embryonic development of an organism. Therefore, this study aims to investigate expression pattern of Grb7 throughout the development of zebrafish, whose cell migration process during embryonic development is well-understood. Semi-quantitative RT-PCR was employed to examine the pattern of Grb7 expression in twelve stages of zebrafish development. The results showed that the gene was expressed in all of the stages studied, although the levels of expression differed among the stages. Comparisons with previous studies revealed that the temporal expression pattern of Grb7 corresponded to that of some of its known binding partners, including PDGFR, EGFR and IR. This suggests the possible occurrence of interactions between Grb7 and these molecules during the process of development in zebrafish |
| first_indexed | 2025-11-15T06:11:05Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-5331 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T06:11:05Z |
| publishDate | 2011 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-53312024-05-02T08:36:25Z http://ir.unimas.my/id/eprint/5331/ Molecular cloning and expression study of growth-factor receptor bound protein 7 (grb7) gene in zebrafish development Tan, Shing Cheng QL Zoology Grb7 is a multi-domain adaptor protein which has been postulated to play a role in the regulation of cell migration. The ability to regulate cell migration implies that the protein may be an important component in the progression of cancer as well as in the embryonic development of an organism. Therefore, this study aims to investigate expression pattern of Grb7 throughout the development of zebrafish, whose cell migration process during embryonic development is well-understood. Semi-quantitative RT-PCR was employed to examine the pattern of Grb7 expression in twelve stages of zebrafish development. The results showed that the gene was expressed in all of the stages studied, although the levels of expression differed among the stages. Comparisons with previous studies revealed that the temporal expression pattern of Grb7 corresponded to that of some of its known binding partners, including PDGFR, EGFR and IR. This suggests the possible occurrence of interactions between Grb7 and these molecules during the process of development in zebrafish Universiti Malaysia Sarawak, (UNIMAS) 2011 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/5331/1/Shing%20%2824pgs%29.pdf text en http://ir.unimas.my/id/eprint/5331/3/Tan%20Shing%20Cheng.pdf Tan, Shing Cheng (2011) Molecular cloning and expression study of growth-factor receptor bound protein 7 (grb7) gene in zebrafish development. [Final Year Project Report / IMRAD] |
| spellingShingle | QL Zoology Tan, Shing Cheng Molecular cloning and expression study of growth-factor receptor bound protein 7 (grb7) gene in zebrafish development |
| title | Molecular cloning and expression study of growth-factor receptor bound protein 7 (grb7) gene in zebrafish development |
| title_full | Molecular cloning and expression study of growth-factor receptor bound protein 7 (grb7) gene in zebrafish development |
| title_fullStr | Molecular cloning and expression study of growth-factor receptor bound protein 7 (grb7) gene in zebrafish development |
| title_full_unstemmed | Molecular cloning and expression study of growth-factor receptor bound protein 7 (grb7) gene in zebrafish development |
| title_short | Molecular cloning and expression study of growth-factor receptor bound protein 7 (grb7) gene in zebrafish development |
| title_sort | molecular cloning and expression study of growth-factor receptor bound protein 7 (grb7) gene in zebrafish development |
| topic | QL Zoology |
| url | http://ir.unimas.my/id/eprint/5331/ http://ir.unimas.my/id/eprint/5331/1/Shing%20%2824pgs%29.pdf http://ir.unimas.my/id/eprint/5331/3/Tan%20Shing%20Cheng.pdf |