Isolation and identification of carbazole-degrading bacteria from marine environment

Carbazole is a significant environmental pollutant and represents a severe health threat. Many coastal areas that are contaminated by petroleum hydrocarbon are heavily impacted by carbazole. This study was conducted to isolate CAR-degrading bacteria from marine environment. Seawater samples were col...

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Bibliographic Details
Main Author: Patsy, Rani
Format: Final Year Project Report / IMRAD
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2011
Subjects:
Online Access:http://ir.unimas.my/id/eprint/5324/
http://ir.unimas.my/id/eprint/5324/1/Patsy.pdf
http://ir.unimas.my/id/eprint/5324/4/Patsy%20Rani%20ft.pdf
Description
Summary:Carbazole is a significant environmental pollutant and represents a severe health threat. Many coastal areas that are contaminated by petroleum hydrocarbon are heavily impacted by carbazole. This study was conducted to isolate CAR-degrading bacteria from marine environment. Seawater samples were collected from Miri, Sarawak and the presence of marine CAR-degrading bacteria was confirmed through enrichment culture of the seawater sample. Isolate P1 was characterized using phenotypic and molecular techniques. The blast result suggested that P1 is a Thalassospira profundimaris with 97% identity similarity. T.profundimaris is not an established marine CAR-degrader. However, T. profundimaris and their close relatives were frequently detected in petrol-oil-degrading consortia which indicate they may play some role in carbazole degradation.