DNA barcoding of kelampayan (Neolamarkia cadamba) progenies (half sib family) using ISSR markers
Neolamarckia cadamba (Kelampayan) is a multipurpose and fast growing plantation tree species. Nowadays, the research done on the Kelampayan trees is very limited. In this study, DNA barcoding of Kelampayan was carried out using the inter simple sequence repeat (ISSR) markers. The DNA was extracted f...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, (UNIMAS)
2011
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| Online Access: | http://ir.unimas.my/id/eprint/5305/ http://ir.unimas.my/id/eprint/5305/8/DNA%20barcoding%20of%20Kelampayan%20%28Neolamarckia%20cadamba%29...%28fulltext%29.pdf |
| _version_ | 1848835633589518336 |
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| author | Moh, Ivy Heng Shi |
| author_facet | Moh, Ivy Heng Shi |
| author_sort | Moh, Ivy Heng Shi |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Neolamarckia cadamba (Kelampayan) is a multipurpose and fast growing plantation tree species. Nowadays, the research done on the Kelampayan trees is very limited. In this study, DNA barcoding of Kelampayan was carried out using the inter simple sequence repeat (ISSR) markers. The DNA was extracted from the plant leaves using fasTip-X method. Three ISSR primers which are (ACC)6G, (AC)8G and (AC)8T were used to determine the genetic relatedness of the Kelampayan progenies (half-sib family). A total of 94 loci were generated in order to determine the genetic relatedness for the progenies. Data analysis was carried out using NTSYS-pc and dendrogram was constructed using UPGMA method. The unrooted neighbour-joining tree was constructed also in order to get a more accurate result. From the dendograms and unrooted neighbourjoining trees constructed, the mother trees and progenies were been grouped into two clusters, cluster I and cluster II. The average similarity based on Jaccard’s similarity coefficient calculated was in the range of 0.433 to 0.584. This indicate that there are no self pollination occur for the Kelampayan. The ISSR markers were successfully been used to determine the genetic relatedness of Kelampayan mother trees and progenies. |
| first_indexed | 2025-11-15T06:10:58Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-5305 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:10:58Z |
| publishDate | 2011 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-53052023-08-28T08:44:04Z http://ir.unimas.my/id/eprint/5305/ DNA barcoding of kelampayan (Neolamarkia cadamba) progenies (half sib family) using ISSR markers Moh, Ivy Heng Shi SD Forestry Neolamarckia cadamba (Kelampayan) is a multipurpose and fast growing plantation tree species. Nowadays, the research done on the Kelampayan trees is very limited. In this study, DNA barcoding of Kelampayan was carried out using the inter simple sequence repeat (ISSR) markers. The DNA was extracted from the plant leaves using fasTip-X method. Three ISSR primers which are (ACC)6G, (AC)8G and (AC)8T were used to determine the genetic relatedness of the Kelampayan progenies (half-sib family). A total of 94 loci were generated in order to determine the genetic relatedness for the progenies. Data analysis was carried out using NTSYS-pc and dendrogram was constructed using UPGMA method. The unrooted neighbour-joining tree was constructed also in order to get a more accurate result. From the dendograms and unrooted neighbourjoining trees constructed, the mother trees and progenies were been grouped into two clusters, cluster I and cluster II. The average similarity based on Jaccard’s similarity coefficient calculated was in the range of 0.433 to 0.584. This indicate that there are no self pollination occur for the Kelampayan. The ISSR markers were successfully been used to determine the genetic relatedness of Kelampayan mother trees and progenies. Universiti Malaysia Sarawak, (UNIMAS) 2011 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/5305/8/DNA%20barcoding%20of%20Kelampayan%20%28Neolamarckia%20cadamba%29...%28fulltext%29.pdf Moh, Ivy Heng Shi (2011) DNA barcoding of kelampayan (Neolamarkia cadamba) progenies (half sib family) using ISSR markers. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | SD Forestry Moh, Ivy Heng Shi DNA barcoding of kelampayan (Neolamarkia cadamba) progenies (half sib family) using ISSR markers |
| title | DNA barcoding of kelampayan (Neolamarkia cadamba) progenies (half sib family) using ISSR markers |
| title_full | DNA barcoding of kelampayan (Neolamarkia cadamba) progenies (half sib family) using ISSR markers |
| title_fullStr | DNA barcoding of kelampayan (Neolamarkia cadamba) progenies (half sib family) using ISSR markers |
| title_full_unstemmed | DNA barcoding of kelampayan (Neolamarkia cadamba) progenies (half sib family) using ISSR markers |
| title_short | DNA barcoding of kelampayan (Neolamarkia cadamba) progenies (half sib family) using ISSR markers |
| title_sort | dna barcoding of kelampayan (neolamarkia cadamba) progenies (half sib family) using issr markers |
| topic | SD Forestry |
| url | http://ir.unimas.my/id/eprint/5305/ http://ir.unimas.my/id/eprint/5305/8/DNA%20barcoding%20of%20Kelampayan%20%28Neolamarckia%20cadamba%29...%28fulltext%29.pdf |