Prevalence and determination of chloramphenicol-resistant gene in Escerichia coli isolated from aquacultural environment
Aquaculture nowadays seems as a promising sector, both in food production and economic intention. However, recently the public health hazards related to antimicrobial use in aquaculture were become a major concern regarding this sector; including the development and spread of antimicrobial resistan...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak (UNIMAS)
2012
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| Online Access: | http://ir.unimas.my/id/eprint/5075/ http://ir.unimas.my/id/eprint/5075/3/Prevalence%20and%20determination%20of%20Chloramphenicol-resistant%20Gene%20in%20Escerichia%20coli%20isolated%20from%20aquacultural%20environment.pdf |
| _version_ | 1848835572514160640 |
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| author | Mohamad Al Aamin, Razali |
| author_facet | Mohamad Al Aamin, Razali |
| author_sort | Mohamad Al Aamin, Razali |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Aquaculture nowadays seems as a promising sector, both in food production and economic intention. However, recently the public health hazards related to antimicrobial use in
aquaculture were become a major concern regarding this sector; including the development and spread of antimicrobial resistant bacteria and resistance genes, and the occurrence of antimicrobial residues in products of aquaculture. Driven by above factor, the main
intention ofthis study is to determine the prevalence of antiobiotic-resistance gene selected bacterial strains from aquaculture environment. Escherichia coli isolates from aquaculture origin were grown on selective media, EMB agar. These isolatess were screened for antibiotic resistance by antibiotic susceptibility test using chloramphenicol (30 Jlg) disk and the respective chloramphenicol-resistance gene in the isolates then were further detected by multiplex PCR, using primers C-l, C-2, C-3, C-4 and C-R for cat I, cat II, cat III, and cat
IV genes respectively in a single reaction mixture. From all 19 isolates, 7 were resistance to chloramphenicol, and only 4 isolates indicates the presences of cat gene during agarose gel electrophoreses, which is cat II gene. The presence of cat gene in this study indicates the
potential chloramphenicol antimicrobial resistance hazard to consumers and aqualculture workers. Further studied should be carried out to determine the origin and the resistance mechanism ofcat gene in the studied isolates. |
| first_indexed | 2025-11-15T06:10:00Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-5075 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:10:00Z |
| publishDate | 2012 |
| publisher | Universiti Malaysia Sarawak (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-50752023-08-25T08:01:53Z http://ir.unimas.my/id/eprint/5075/ Prevalence and determination of chloramphenicol-resistant gene in Escerichia coli isolated from aquacultural environment Mohamad Al Aamin, Razali SH Aquaculture. Fisheries. Angling Aquaculture nowadays seems as a promising sector, both in food production and economic intention. However, recently the public health hazards related to antimicrobial use in aquaculture were become a major concern regarding this sector; including the development and spread of antimicrobial resistant bacteria and resistance genes, and the occurrence of antimicrobial residues in products of aquaculture. Driven by above factor, the main intention ofthis study is to determine the prevalence of antiobiotic-resistance gene selected bacterial strains from aquaculture environment. Escherichia coli isolates from aquaculture origin were grown on selective media, EMB agar. These isolatess were screened for antibiotic resistance by antibiotic susceptibility test using chloramphenicol (30 Jlg) disk and the respective chloramphenicol-resistance gene in the isolates then were further detected by multiplex PCR, using primers C-l, C-2, C-3, C-4 and C-R for cat I, cat II, cat III, and cat IV genes respectively in a single reaction mixture. From all 19 isolates, 7 were resistance to chloramphenicol, and only 4 isolates indicates the presences of cat gene during agarose gel electrophoreses, which is cat II gene. The presence of cat gene in this study indicates the potential chloramphenicol antimicrobial resistance hazard to consumers and aqualculture workers. Further studied should be carried out to determine the origin and the resistance mechanism ofcat gene in the studied isolates. Universiti Malaysia Sarawak (UNIMAS) 2012 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/5075/3/Prevalence%20and%20determination%20of%20Chloramphenicol-resistant%20Gene%20in%20Escerichia%20coli%20isolated%20from%20aquacultural%20environment.pdf Mohamad Al Aamin, Razali (2012) Prevalence and determination of chloramphenicol-resistant gene in Escerichia coli isolated from aquacultural environment. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | SH Aquaculture. Fisheries. Angling Mohamad Al Aamin, Razali Prevalence and determination of chloramphenicol-resistant gene in Escerichia coli isolated from aquacultural environment |
| title | Prevalence and determination of chloramphenicol-resistant gene in Escerichia coli isolated from aquacultural environment |
| title_full | Prevalence and determination of chloramphenicol-resistant gene in Escerichia coli isolated from aquacultural environment |
| title_fullStr | Prevalence and determination of chloramphenicol-resistant gene in Escerichia coli isolated from aquacultural environment |
| title_full_unstemmed | Prevalence and determination of chloramphenicol-resistant gene in Escerichia coli isolated from aquacultural environment |
| title_short | Prevalence and determination of chloramphenicol-resistant gene in Escerichia coli isolated from aquacultural environment |
| title_sort | prevalence and determination of chloramphenicol-resistant gene in escerichia coli isolated from aquacultural environment |
| topic | SH Aquaculture. Fisheries. Angling |
| url | http://ir.unimas.my/id/eprint/5075/ http://ir.unimas.my/id/eprint/5075/3/Prevalence%20and%20determination%20of%20Chloramphenicol-resistant%20Gene%20in%20Escerichia%20coli%20isolated%20from%20aquacultural%20environment.pdf |