Production of Kojic acid from sago hampas using combination of different aspergillus strains via solid state fermentation
Kojic acid (5-hydroxy-2-hydroxymethyl-4-pyrone) is a depigmenting agent produced by several species of fungi, particularly Aspergillus flavus. In this work, the production of kojic acid using combination of different Aspergillus strains was investigated. Three sets of fermentation were carried ou...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, UNIMAS
2013
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/4459/ http://ir.unimas.my/id/eprint/4459/7/Production%20of%20Kojic%20acid%20%28fulltext%29.pdf |
| Summary: | Kojic acid (5-hydroxy-2-hydroxymethyl-4-pyrone) is a depigmenting agent produced by
several species of fungi, particularly Aspergillus flavus. In this work, the production of kojic
acid using combination of different Aspergillus strains was investigated. Three sets of
fermentation were carried out using individual culture of A. flavus Link 44-1, A. flavus NSH9
and combination of both strains in a culture. Single-strain A.flavus Link 44-1 at inoculum size
of 107
spores/ml exhibit the highest production of kojic acid which is 9.5 g/L. This result
indicates the feasibility of single-strain A. flavus Link 44-1 as potential strain for the
production of kojic acid using sago hampas as substrate. The effects of different spore
concentration ranging from 105 spores/ ml to 108
spores/ ml on kojic acid production was
tested. Throughout this work, the potential of utilizing multi-strains as an alternative to
single-strain inoculum for kojic acid fermentation was examined. |
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