Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM

The axenic seedlings were kept in vitro as the source axenic explants. Shoot-tip explants derived from axenic off shoots were cultured in Gamborg B5 media incorporated with 30% sucrose, 3 g/L gelrite and different concentrations of cytokinins i.e. TDZ, BAP alone or in combination with NAA in the dir...

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Main Author: Bay, Hui Han
Format: Thesis
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2008
Subjects:
Online Access:http://ir.unimas.my/id/eprint/3588/
http://ir.unimas.my/id/eprint/3588/2/Bay%20Hui%20Han%20ft.pdf
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author Bay, Hui Han
author_facet Bay, Hui Han
author_sort Bay, Hui Han
building UNIMAS Institutional Repository
collection Online Access
description The axenic seedlings were kept in vitro as the source axenic explants. Shoot-tip explants derived from axenic off shoots were cultured in Gamborg B5 media incorporated with 30% sucrose, 3 g/L gelrite and different concentrations of cytokinins i.e. TDZ, BAP alone or in combination with NAA in the direct regeneration pathway. The primers produced amplification products which were monomorphic across the mother plants and the micropropagated plantlets, confirming the reliability of developed micropropagation protocol to produce true-to-tpe plantlets. Both leaves and leaf sheaths were used for callus induction in the indirect regeneration pathway.
first_indexed 2025-11-15T06:04:58Z
format Thesis
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institution Universiti Malaysia Sarawak
institution_category Local University
language English
last_indexed 2025-11-15T06:04:58Z
publishDate 2008
publisher Universiti Malaysia Sarawak, (UNIMAS)
recordtype eprints
repository_type Digital Repository
spelling unimas-35882025-06-12T08:00:42Z http://ir.unimas.my/id/eprint/3588/ Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM Bay, Hui Han QK Botany The axenic seedlings were kept in vitro as the source axenic explants. Shoot-tip explants derived from axenic off shoots were cultured in Gamborg B5 media incorporated with 30% sucrose, 3 g/L gelrite and different concentrations of cytokinins i.e. TDZ, BAP alone or in combination with NAA in the direct regeneration pathway. The primers produced amplification products which were monomorphic across the mother plants and the micropropagated plantlets, confirming the reliability of developed micropropagation protocol to produce true-to-tpe plantlets. Both leaves and leaf sheaths were used for callus induction in the indirect regeneration pathway. Universiti Malaysia Sarawak, (UNIMAS) 2008 Thesis NonPeerReviewed text en http://ir.unimas.my/id/eprint/3588/2/Bay%20Hui%20Han%20ft.pdf Bay, Hui Han (2008) Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM. Masters thesis, Universiti Malaysia Sarawak (UNIMAS).
spellingShingle QK Botany
Bay, Hui Han
Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM
title Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM
title_full Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM
title_fullStr Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM
title_full_unstemmed Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM
title_short Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM
title_sort micropropagation and monitoring of genetic stability of micropagated plantlets of hornstedtia reticulata k. schum
topic QK Botany
url http://ir.unimas.my/id/eprint/3588/
http://ir.unimas.my/id/eprint/3588/2/Bay%20Hui%20Han%20ft.pdf