Nucleotide polymorphism of sucrose synthase (susy) from kelampayan (neolamarckia cadamba)
Neolamarckia cadamba has high commercial value as its timber used as raw materials for production of plywood, pulp and paper, boxes and crates, light construction, dug-out canoes and furniture. Sucrose synthase (SuSy) catalyzes reversible formation of sucrose (Suc) and uridine diphosphate (UDP) into...
| Main Author: | |
|---|---|
| Format: | Final Year Project Report / IMRAD |
| Language: | English English |
| Published: |
University Malaysia Sarawak, (UNIMAS)
2013
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/3279/ http://ir.unimas.my/id/eprint/3279/1/Chong%20Hui%20Nei%20ft.pdf http://ir.unimas.my/id/eprint/3279/3/Nucleotide%20polymorphism%20of%20Sucrose%20Synthase%20%28Susy%29%20from%20Kelampayan%20%28Neolamarckia%20Cadamba%29%20%28fulltext%29.pdf |
| _version_ | 1848835177233514496 |
|---|---|
| author | Chong, Hui Nei |
| author_facet | Chong, Hui Nei |
| author_sort | Chong, Hui Nei |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Neolamarckia cadamba has high commercial value as its timber used as raw materials for production of plywood, pulp and paper, boxes and crates, light construction, dug-out canoes and furniture. Sucrose synthase (SuSy) catalyzes reversible formation of sucrose (Suc) and uridine diphosphate (UDP) into fructose (fru) and UDP-glucose (UDPG). UDPG is a major glycosyl donor for biosynthesis of cellulose, callose and starch. Single nucleotide polymorphism (SNP) is an important marker for selective breeding in the selection of desirable traits without growing plant until mature to observe phenotypic traits. The aim of this study was to identify the presence of SNP(s) in SuSy partial genomic sequence of Kelampayan. Targeted SuSy sequences were amplified by polymerase chain reaction (PCR). The ~800bp of SuSy partial genomic sequences was subjected to BLASTn analysis to search for sequence homology in NCBI. Sequences were then aligned by using CLC Sequence Viewer 6 software to identify SNP(s). There were 37 SNPs and 2 InDel polymorphisms detected in partial sequences of sucrose synthase. |
| first_indexed | 2025-11-15T06:03:43Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-3279 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T06:03:43Z |
| publishDate | 2013 |
| publisher | University Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-32792023-01-30T09:34:24Z http://ir.unimas.my/id/eprint/3279/ Nucleotide polymorphism of sucrose synthase (susy) from kelampayan (neolamarckia cadamba) Chong, Hui Nei Q Science (General) SD Forestry Neolamarckia cadamba has high commercial value as its timber used as raw materials for production of plywood, pulp and paper, boxes and crates, light construction, dug-out canoes and furniture. Sucrose synthase (SuSy) catalyzes reversible formation of sucrose (Suc) and uridine diphosphate (UDP) into fructose (fru) and UDP-glucose (UDPG). UDPG is a major glycosyl donor for biosynthesis of cellulose, callose and starch. Single nucleotide polymorphism (SNP) is an important marker for selective breeding in the selection of desirable traits without growing plant until mature to observe phenotypic traits. The aim of this study was to identify the presence of SNP(s) in SuSy partial genomic sequence of Kelampayan. Targeted SuSy sequences were amplified by polymerase chain reaction (PCR). The ~800bp of SuSy partial genomic sequences was subjected to BLASTn analysis to search for sequence homology in NCBI. Sequences were then aligned by using CLC Sequence Viewer 6 software to identify SNP(s). There were 37 SNPs and 2 InDel polymorphisms detected in partial sequences of sucrose synthase. University Malaysia Sarawak, (UNIMAS) 2013 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/3279/1/Chong%20Hui%20Nei%20ft.pdf text en http://ir.unimas.my/id/eprint/3279/3/Nucleotide%20polymorphism%20of%20Sucrose%20Synthase%20%28Susy%29%20from%20Kelampayan%20%28Neolamarckia%20Cadamba%29%20%28fulltext%29.pdf Chong, Hui Nei (2013) Nucleotide polymorphism of sucrose synthase (susy) from kelampayan (neolamarckia cadamba). [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | Q Science (General) SD Forestry Chong, Hui Nei Nucleotide polymorphism of sucrose synthase (susy) from kelampayan (neolamarckia cadamba) |
| title | Nucleotide polymorphism of sucrose synthase (susy) from kelampayan (neolamarckia cadamba) |
| title_full | Nucleotide polymorphism of sucrose synthase (susy) from kelampayan (neolamarckia cadamba) |
| title_fullStr | Nucleotide polymorphism of sucrose synthase (susy) from kelampayan (neolamarckia cadamba) |
| title_full_unstemmed | Nucleotide polymorphism of sucrose synthase (susy) from kelampayan (neolamarckia cadamba) |
| title_short | Nucleotide polymorphism of sucrose synthase (susy) from kelampayan (neolamarckia cadamba) |
| title_sort | nucleotide polymorphism of sucrose synthase (susy) from kelampayan (neolamarckia cadamba) |
| topic | Q Science (General) SD Forestry |
| url | http://ir.unimas.my/id/eprint/3279/ http://ir.unimas.my/id/eprint/3279/1/Chong%20Hui%20Nei%20ft.pdf http://ir.unimas.my/id/eprint/3279/3/Nucleotide%20polymorphism%20of%20Sucrose%20Synthase%20%28Susy%29%20from%20Kelampayan%20%28Neolamarckia%20Cadamba%29%20%28fulltext%29.pdf |