Application of the real-time polymerase chain reaction in the detection and enumeration of vibrio cholera from shrimp ponds
Cholera is an enteric disease which causes severe pandemics and it is caused by a bacterium known as Vibrio cholerae. This project focused on the use of real-time PCR to detect and enumerate the presence of V. cholerae from selected shrimp ponds from Asia Aquaculture Sdn. Bhd in Bako Kuching, Sarawa...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, UNIMAS
2013
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| Online Access: | http://ir.unimas.my/id/eprint/3274/ http://ir.unimas.my/id/eprint/3274/13/Shafillah%20%28fulltext%29.pdf |
| _version_ | 1848835176996536320 |
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| author | Shafillah, binti Sulaiman |
| author_facet | Shafillah, binti Sulaiman |
| author_sort | Shafillah, binti Sulaiman |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Cholera is an enteric disease which causes severe pandemics and it is caused by a bacterium known as Vibrio cholerae. This project focused on the use of real-time PCR to detect and enumerate the presence of V. cholerae from selected shrimp ponds from Asia Aquaculture Sdn. Bhd in Bako Kuching, Sarawak. In this research, repeat in toxin (rtxA) gene was used as the target gene of V. cholerae and the presence of V. cholerae were detected and enumerated of V. cholerae also been done by Real-time PCR. The dynamics of V. cholerae shows a decreasing population during culturing period. The results showed that the V. cholerae population densities was on a decreasing trend with the R2 value in Pond 1 at 0.85,
meanwhile R2 value in Pond 14 at 0.68. Thus, it shows a decreasing trend for both shrimp ponds concentration of V. cholerae throughout culturing period. It is believed that real-time PCR assay allows for more rapid detection and identification of V. cholerae which is faster than current conventional assay. |
| first_indexed | 2025-11-15T06:03:43Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-3274 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:03:43Z |
| publishDate | 2013 |
| publisher | Universiti Malaysia Sarawak, UNIMAS |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-32742024-02-06T03:49:38Z http://ir.unimas.my/id/eprint/3274/ Application of the real-time polymerase chain reaction in the detection and enumeration of vibrio cholera from shrimp ponds Shafillah, binti Sulaiman QH426 Genetics T Technology (General) Cholera is an enteric disease which causes severe pandemics and it is caused by a bacterium known as Vibrio cholerae. This project focused on the use of real-time PCR to detect and enumerate the presence of V. cholerae from selected shrimp ponds from Asia Aquaculture Sdn. Bhd in Bako Kuching, Sarawak. In this research, repeat in toxin (rtxA) gene was used as the target gene of V. cholerae and the presence of V. cholerae were detected and enumerated of V. cholerae also been done by Real-time PCR. The dynamics of V. cholerae shows a decreasing population during culturing period. The results showed that the V. cholerae population densities was on a decreasing trend with the R2 value in Pond 1 at 0.85, meanwhile R2 value in Pond 14 at 0.68. Thus, it shows a decreasing trend for both shrimp ponds concentration of V. cholerae throughout culturing period. It is believed that real-time PCR assay allows for more rapid detection and identification of V. cholerae which is faster than current conventional assay. Universiti Malaysia Sarawak, UNIMAS 2013 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/3274/13/Shafillah%20%28fulltext%29.pdf Shafillah, binti Sulaiman (2013) Application of the real-time polymerase chain reaction in the detection and enumeration of vibrio cholera from shrimp ponds. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | QH426 Genetics T Technology (General) Shafillah, binti Sulaiman Application of the real-time polymerase chain reaction in the detection and enumeration of vibrio cholera from shrimp ponds |
| title | Application of the real-time polymerase chain reaction in the detection and enumeration of vibrio cholera from shrimp ponds |
| title_full | Application of the real-time polymerase chain reaction in the detection and enumeration of vibrio cholera from shrimp ponds |
| title_fullStr | Application of the real-time polymerase chain reaction in the detection and enumeration of vibrio cholera from shrimp ponds |
| title_full_unstemmed | Application of the real-time polymerase chain reaction in the detection and enumeration of vibrio cholera from shrimp ponds |
| title_short | Application of the real-time polymerase chain reaction in the detection and enumeration of vibrio cholera from shrimp ponds |
| title_sort | application of the real-time polymerase chain reaction in the detection and enumeration of vibrio cholera from shrimp ponds |
| topic | QH426 Genetics T Technology (General) |
| url | http://ir.unimas.my/id/eprint/3274/ http://ir.unimas.my/id/eprint/3274/13/Shafillah%20%28fulltext%29.pdf |