Occurrence of Leptospira sp. In environmental water samples from wind cave, Bau, Sarawak
The genus Leptospira is composed of tightly coiled spirochetes that exist in two groups which are pathogenic strains and saprophytic strains. The pathogenic strains caused Leptospirosis, the most widespread zoonotic disease which is also known as the rat urine’s disease. The saprophytic strains are...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, UNIMAS
2013
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| Online Access: | http://ir.unimas.my/id/eprint/3242/ http://ir.unimas.my/id/eprint/3242/9/Occurrence%20of%20leptospira..%28fulltext%29.pdf |
| _version_ | 1848835169396457472 |
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| author | Irwin Emmanuel, ak Robin Jeli |
| author_facet | Irwin Emmanuel, ak Robin Jeli |
| author_sort | Irwin Emmanuel, ak Robin Jeli |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | The genus Leptospira is composed of tightly coiled spirochetes that exist in two groups which are pathogenic strains and saprophytic strains. The pathogenic strains caused Leptospirosis, the most widespread zoonotic disease which is also known as the rat urine’s disease. The saprophytic strains are usually found on surface water meanwhile the pathogenic strains requires animal host to survive. The aim of this research is determine the occurrence of Leptospira sp. isolated from environmental water samples collected from Wind Cave, Bau. A total of 17 water samples were collected from the sampling site. The samples were cultured in EMJH medium for 30 days and were observed under dark field microscope for the presence of Leptospira. No observation of leptospires was detected. Six samples were selected for comparison purposes. Further confirmation was done by utilizing polymerase chain reaction targeting the LipL32 gene. The findings reported that LipL32 gene was successfully amplified by PCR in three of the samples. Therefore, the use of PCR by targeting the LipL32 gene is more reliable compared to direct observation under dark field microscope in detecting Leptopsira in environmental water. |
| first_indexed | 2025-11-15T06:03:36Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-3242 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:03:36Z |
| publishDate | 2013 |
| publisher | Universiti Malaysia Sarawak, UNIMAS |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-32422023-06-15T08:34:11Z http://ir.unimas.my/id/eprint/3242/ Occurrence of Leptospira sp. In environmental water samples from wind cave, Bau, Sarawak Irwin Emmanuel, ak Robin Jeli GE Environmental Sciences The genus Leptospira is composed of tightly coiled spirochetes that exist in two groups which are pathogenic strains and saprophytic strains. The pathogenic strains caused Leptospirosis, the most widespread zoonotic disease which is also known as the rat urine’s disease. The saprophytic strains are usually found on surface water meanwhile the pathogenic strains requires animal host to survive. The aim of this research is determine the occurrence of Leptospira sp. isolated from environmental water samples collected from Wind Cave, Bau. A total of 17 water samples were collected from the sampling site. The samples were cultured in EMJH medium for 30 days and were observed under dark field microscope for the presence of Leptospira. No observation of leptospires was detected. Six samples were selected for comparison purposes. Further confirmation was done by utilizing polymerase chain reaction targeting the LipL32 gene. The findings reported that LipL32 gene was successfully amplified by PCR in three of the samples. Therefore, the use of PCR by targeting the LipL32 gene is more reliable compared to direct observation under dark field microscope in detecting Leptopsira in environmental water. Universiti Malaysia Sarawak, UNIMAS 2013 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/3242/9/Occurrence%20of%20leptospira..%28fulltext%29.pdf Irwin Emmanuel, ak Robin Jeli (2013) Occurrence of Leptospira sp. In environmental water samples from wind cave, Bau, Sarawak. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | GE Environmental Sciences Irwin Emmanuel, ak Robin Jeli Occurrence of Leptospira sp. In environmental water samples from wind cave, Bau, Sarawak |
| title | Occurrence of Leptospira sp. In environmental water samples from wind cave, Bau, Sarawak |
| title_full | Occurrence of Leptospira sp. In environmental water samples from wind cave, Bau, Sarawak |
| title_fullStr | Occurrence of Leptospira sp. In environmental water samples from wind cave, Bau, Sarawak |
| title_full_unstemmed | Occurrence of Leptospira sp. In environmental water samples from wind cave, Bau, Sarawak |
| title_short | Occurrence of Leptospira sp. In environmental water samples from wind cave, Bau, Sarawak |
| title_sort | occurrence of leptospira sp. in environmental water samples from wind cave, bau, sarawak |
| topic | GE Environmental Sciences |
| url | http://ir.unimas.my/id/eprint/3242/ http://ir.unimas.my/id/eprint/3242/9/Occurrence%20of%20leptospira..%28fulltext%29.pdf |