Optimization of dna extraction from orang utan using mitochondrial cytochrome b gene
This study examines the optimization of DNA extraction from Orang Utans' hair samples. The DNA sequence analyses were carried out using mitochondrial Cytochrome b gene. A total of 8 samples of Orang Utan were used in this study, which were collected from the Matang Wildlife Centre. The metho...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English English |
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Universiti Malaysia Sarawak, (UNIMAS)
2011
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/316/ http://ir.unimas.my/id/eprint/316/1/Kennedy.pdf http://ir.unimas.my/id/eprint/316/4/Kennedy%20Lim%20ft.pdf |
| _version_ | 1848834515012681728 |
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| author | Lim, Kennedy Chong Ming |
| author_facet | Lim, Kennedy Chong Ming |
| author_sort | Lim, Kennedy Chong Ming |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | This study examines the optimization of DNA extraction from Orang Utans' hair samples. The DNA sequence analyses were carried out using mitochondrial Cytochrome b gene. A total of 8 samples of Orang Utan were used in this study, which were collected from the Matang Wildlife Centre. The methods used were conventional CTAB method and commercial DNA Extraction Kit (Promega). The results showed that extraction was possible using both methods but the DNAs present were contaminated with organic materials. Due to the contaminants and insufficient DNA concentration, optimization of the extracted DNA was not successfully achieved. |
| first_indexed | 2025-11-15T05:53:12Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-316 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T05:53:12Z |
| publishDate | 2011 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-3162024-03-07T03:54:46Z http://ir.unimas.my/id/eprint/316/ Optimization of dna extraction from orang utan using mitochondrial cytochrome b gene Lim, Kennedy Chong Ming Q Science (General) S Agriculture (General) SF Animal culture This study examines the optimization of DNA extraction from Orang Utans' hair samples. The DNA sequence analyses were carried out using mitochondrial Cytochrome b gene. A total of 8 samples of Orang Utan were used in this study, which were collected from the Matang Wildlife Centre. The methods used were conventional CTAB method and commercial DNA Extraction Kit (Promega). The results showed that extraction was possible using both methods but the DNAs present were contaminated with organic materials. Due to the contaminants and insufficient DNA concentration, optimization of the extracted DNA was not successfully achieved. Universiti Malaysia Sarawak, (UNIMAS) 2011 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/316/1/Kennedy.pdf text en http://ir.unimas.my/id/eprint/316/4/Kennedy%20Lim%20ft.pdf Lim, Kennedy Chong Ming (2011) Optimization of dna extraction from orang utan using mitochondrial cytochrome b gene. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | Q Science (General) S Agriculture (General) SF Animal culture Lim, Kennedy Chong Ming Optimization of dna extraction from orang utan using mitochondrial cytochrome b gene |
| title | Optimization of dna extraction from orang utan using mitochondrial cytochrome b gene |
| title_full | Optimization of dna extraction from orang utan using mitochondrial cytochrome b gene |
| title_fullStr | Optimization of dna extraction from orang utan using mitochondrial cytochrome b gene |
| title_full_unstemmed | Optimization of dna extraction from orang utan using mitochondrial cytochrome b gene |
| title_short | Optimization of dna extraction from orang utan using mitochondrial cytochrome b gene |
| title_sort | optimization of dna extraction from orang utan using mitochondrial cytochrome b gene |
| topic | Q Science (General) S Agriculture (General) SF Animal culture |
| url | http://ir.unimas.my/id/eprint/316/ http://ir.unimas.my/id/eprint/316/1/Kennedy.pdf http://ir.unimas.my/id/eprint/316/4/Kennedy%20Lim%20ft.pdf |