Reconstruction of msadh gene
Alcohol dehydrogenase (Adh) genes are highly characterized in alcoholic pathway which normally occurs in most of plants. These genes are activated when under anaerobic stresses by producing measurable level of alcohol dehydrogenase enzyme which is capable in converting acetaldehyde into relativel...
| Main Author: | |
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2011
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/314/ http://ir.unimas.my/id/eprint/314/8/Ho%20Carl%20Miew.pdf |
| _version_ | 1848834514750537728 |
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| author | Ho, Carl Miew |
| author_facet | Ho, Carl Miew |
| author_sort | Ho, Carl Miew |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Alcohol dehydrogenase (Adh) genes are highly characterized in alcoholic pathway which
normally occurs in most of plants. These genes are activated when under anaerobic stresses
by producing measurable level of alcohol dehydrogenase enzyme which is capable in
converting acetaldehyde into relatively low toxicity compound, ethanol. This vital response
of plants has been proved in enhancing the potential for survival under hypoxic conditions
with a low production of A TP and regeneration of NAD+· The objective of this study was
to identify and verify the presence of AdhipET inserts within plasmid which is isolated
from Metroxylon sagu via Restriction endonuclease and Polymerase Chain Reaction. In
addition, reconstruction of the msAdh plasmid was carried out due to the frame shift of
single nucleotide. |
| first_indexed | 2025-11-15T05:53:11Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-314 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T05:53:11Z |
| publishDate | 2011 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-3142023-10-31T07:34:05Z http://ir.unimas.my/id/eprint/314/ Reconstruction of msadh gene Ho, Carl Miew Q Science (General) Alcohol dehydrogenase (Adh) genes are highly characterized in alcoholic pathway which normally occurs in most of plants. These genes are activated when under anaerobic stresses by producing measurable level of alcohol dehydrogenase enzyme which is capable in converting acetaldehyde into relatively low toxicity compound, ethanol. This vital response of plants has been proved in enhancing the potential for survival under hypoxic conditions with a low production of A TP and regeneration of NAD+· The objective of this study was to identify and verify the presence of AdhipET inserts within plasmid which is isolated from Metroxylon sagu via Restriction endonuclease and Polymerase Chain Reaction. In addition, reconstruction of the msAdh plasmid was carried out due to the frame shift of single nucleotide. Universiti Malaysia Sarawak, (UNIMAS) 2011 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/314/8/Ho%20Carl%20Miew.pdf Ho, Carl Miew (2011) Reconstruction of msadh gene. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | Q Science (General) Ho, Carl Miew Reconstruction of msadh gene |
| title | Reconstruction of msadh gene |
| title_full | Reconstruction of msadh gene |
| title_fullStr | Reconstruction of msadh gene |
| title_full_unstemmed | Reconstruction of msadh gene |
| title_short | Reconstruction of msadh gene |
| title_sort | reconstruction of msadh gene |
| topic | Q Science (General) |
| url | http://ir.unimas.my/id/eprint/314/ http://ir.unimas.my/id/eprint/314/8/Ho%20Carl%20Miew.pdf |