Transformation of isochorismate synthase in tomato plants
Isochorismate synthase (ICS) is an important enzyme during the conversion of chorismate into isochorismate in the shikimate pathway. The cDNA was transformed into tomato plant to observe the functional and expression activity. The objective of this study is to insert the ICS gene isolated from Morin...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, UNIMAS
2013
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| Online Access: | http://ir.unimas.my/id/eprint/2965/ http://ir.unimas.my/id/eprint/2965/7/Siti%20Nurul%20Ain.pdf |
| _version_ | 1848835113773694976 |
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| author | Sidi, Siti Nurul Ain Binti |
| author_facet | Sidi, Siti Nurul Ain Binti |
| author_sort | Sidi, Siti Nurul Ain Binti |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Isochorismate synthase (ICS) is an important enzyme during the conversion of chorismate into isochorismate in the shikimate pathway. The cDNA was transformed into tomato plant to observe the functional and expression activity. The objective of this study is to insert the ICS gene isolated from Morinda citrifolia into tomato by seed via infiltration method. Mardi Tomato 1 (MT1) seeds were treated with sonication before immersed in infiltration broth on a shaking incubator and subsequently sown into soils. About 887 bp size of ICS cDNA was not successfully transformed into the MT1 plants confirmed by polymerase chain reaction (PCR). |
| first_indexed | 2025-11-15T06:02:43Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-2965 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:02:43Z |
| publishDate | 2013 |
| publisher | Universiti Malaysia Sarawak, UNIMAS |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-29652024-02-05T08:01:25Z http://ir.unimas.my/id/eprint/2965/ Transformation of isochorismate synthase in tomato plants Sidi, Siti Nurul Ain Binti AC Collections. Series. Collected works SB Plant culture T Technology (General) Isochorismate synthase (ICS) is an important enzyme during the conversion of chorismate into isochorismate in the shikimate pathway. The cDNA was transformed into tomato plant to observe the functional and expression activity. The objective of this study is to insert the ICS gene isolated from Morinda citrifolia into tomato by seed via infiltration method. Mardi Tomato 1 (MT1) seeds were treated with sonication before immersed in infiltration broth on a shaking incubator and subsequently sown into soils. About 887 bp size of ICS cDNA was not successfully transformed into the MT1 plants confirmed by polymerase chain reaction (PCR). Universiti Malaysia Sarawak, UNIMAS 2013 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/2965/7/Siti%20Nurul%20Ain.pdf Sidi, Siti Nurul Ain Binti (2013) Transformation of isochorismate synthase in tomato plants. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | AC Collections. Series. Collected works SB Plant culture T Technology (General) Sidi, Siti Nurul Ain Binti Transformation of isochorismate synthase in tomato plants |
| title | Transformation of isochorismate synthase in tomato plants |
| title_full | Transformation of isochorismate synthase in tomato plants |
| title_fullStr | Transformation of isochorismate synthase in tomato plants |
| title_full_unstemmed | Transformation of isochorismate synthase in tomato plants |
| title_short | Transformation of isochorismate synthase in tomato plants |
| title_sort | transformation of isochorismate synthase in tomato plants |
| topic | AC Collections. Series. Collected works SB Plant culture T Technology (General) |
| url | http://ir.unimas.my/id/eprint/2965/ http://ir.unimas.my/id/eprint/2965/7/Siti%20Nurul%20Ain.pdf |