Repeated Batch Fermentation For Lactic Acid Production By Enteroccocus Faecium In Liquefied Sago Starch
Enterococcus faecium No. 78 (BIOTECH 10375) isolated from puto, a type of fermented rice in the Philippines was used to produce lactic acid (LA) in repeated batch fermentation (RBF) mode. Enzymatically liquefied sago starch (LSS) was used as the sole carbon source. LSS was inoculated with E. faecium...
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| Format: | Article |
| Language: | English |
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AMB Express
2012
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| Online Access: | http://ir.unimas.my/id/eprint/2356/ http://ir.unimas.my/id/eprint/2356/1/Repeated%20Batch%20Fermentation%20For%20Lactic%20Acid%20Production%20By%20Enteroccocus%20Faecium%20In%20Liquefied%20Sago%20Starch.pdf |
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| author | Nolasco-Hipolito, Cirilo Zarrabal, Octavio Carvajal Kopli, Bujang Rubena Mafia, Kamaldin Samuel, Lihan Ling, Teck Yee |
| author_facet | Nolasco-Hipolito, Cirilo Zarrabal, Octavio Carvajal Kopli, Bujang Rubena Mafia, Kamaldin Samuel, Lihan Ling, Teck Yee |
| author_sort | Nolasco-Hipolito, Cirilo |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Enterococcus faecium No. 78 (BIOTECH 10375) isolated from puto, a type of fermented rice in the Philippines was used to produce lactic acid (LA) in repeated batch fermentation (RBF) mode. Enzymatically liquefied sago starch (LSS) was used as the sole carbon source. LSS was inoculated with E. faecium to perform the saccharification and fermentation processes simultaneously. Results demonstrated that E. faecium was reused for up to 11 fermentation cycles with an average LA yield of 36.3 g/L obtained in 15 hrs at the end of the log phase with the ability to produce 42.5 g/L LA in 30 hrs. By using RBF mode the strain was able to maintain a volumetric productivity of 1.96±.0.241 g/Lh during more than 280 hrs of fermentation. Work is in progress to maintain and increase the usability of the cells over higher fermentation cycles. |
| first_indexed | 2025-11-15T06:00:22Z |
| format | Article |
| id | unimas-2356 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:00:22Z |
| publishDate | 2012 |
| publisher | AMB Express |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-23562016-04-12T04:02:26Z http://ir.unimas.my/id/eprint/2356/ Repeated Batch Fermentation For Lactic Acid Production By Enteroccocus Faecium In Liquefied Sago Starch Nolasco-Hipolito, Cirilo Zarrabal, Octavio Carvajal Kopli, Bujang Rubena Mafia, Kamaldin Samuel, Lihan Ling, Teck Yee S Agriculture (General) Enterococcus faecium No. 78 (BIOTECH 10375) isolated from puto, a type of fermented rice in the Philippines was used to produce lactic acid (LA) in repeated batch fermentation (RBF) mode. Enzymatically liquefied sago starch (LSS) was used as the sole carbon source. LSS was inoculated with E. faecium to perform the saccharification and fermentation processes simultaneously. Results demonstrated that E. faecium was reused for up to 11 fermentation cycles with an average LA yield of 36.3 g/L obtained in 15 hrs at the end of the log phase with the ability to produce 42.5 g/L LA in 30 hrs. By using RBF mode the strain was able to maintain a volumetric productivity of 1.96±.0.241 g/Lh during more than 280 hrs of fermentation. Work is in progress to maintain and increase the usability of the cells over higher fermentation cycles. AMB Express 2012 Article PeerReviewed text en http://ir.unimas.my/id/eprint/2356/1/Repeated%20Batch%20Fermentation%20For%20Lactic%20Acid%20Production%20By%20Enteroccocus%20Faecium%20In%20Liquefied%20Sago%20Starch.pdf Nolasco-Hipolito, Cirilo and Zarrabal, Octavio Carvajal and Kopli, Bujang and Rubena Mafia, Kamaldin and Samuel, Lihan and Ling, Teck Yee (2012) Repeated Batch Fermentation For Lactic Acid Production By Enteroccocus Faecium In Liquefied Sago Starch. -. http://www.amb-express.com/content/2/1/53 |
| spellingShingle | S Agriculture (General) Nolasco-Hipolito, Cirilo Zarrabal, Octavio Carvajal Kopli, Bujang Rubena Mafia, Kamaldin Samuel, Lihan Ling, Teck Yee Repeated Batch Fermentation For Lactic Acid Production By Enteroccocus Faecium In Liquefied Sago Starch |
| title | Repeated Batch Fermentation For Lactic Acid Production By Enteroccocus Faecium In Liquefied Sago Starch |
| title_full | Repeated Batch Fermentation For Lactic Acid Production By Enteroccocus Faecium In Liquefied Sago Starch |
| title_fullStr | Repeated Batch Fermentation For Lactic Acid Production By Enteroccocus Faecium In Liquefied Sago Starch |
| title_full_unstemmed | Repeated Batch Fermentation For Lactic Acid Production By Enteroccocus Faecium In Liquefied Sago Starch |
| title_short | Repeated Batch Fermentation For Lactic Acid Production By Enteroccocus Faecium In Liquefied Sago Starch |
| title_sort | repeated batch fermentation for lactic acid production by enteroccocus faecium in liquefied sago starch |
| topic | S Agriculture (General) |
| url | http://ir.unimas.my/id/eprint/2356/ http://ir.unimas.my/id/eprint/2356/ http://ir.unimas.my/id/eprint/2356/1/Repeated%20Batch%20Fermentation%20For%20Lactic%20Acid%20Production%20By%20Enteroccocus%20Faecium%20In%20Liquefied%20Sago%20Starch.pdf |