Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by PCR
This study aimed to determine the occurrence of Vibrio parahaemolyticus in cockles (Anadara granosa) at a harvesting area and to detect the presence of virulent strains carrying the thermostable direct hemolysin (tdh) and TDH-related hemolysin genes (trh) using PCR. Of 100 samples, 62 were positive...
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| Format: | Article |
| Language: | English |
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John Wiley & Sons Ltd
2005
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| Online Access: | http://ir.unimas.my/id/eprint/2128/ http://ir.unimas.my/id/eprint/2128/1/Detection%20of%20Vibrio%20parahaemolyticus%20in%20cockle%20%28Anadara%20granosa%29%20by%20PCR.pdf |
| _version_ | 1848834914893430784 |
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| author | Lesley Maurice, Bilung Son, Radu Abdul Rani, Bahaman Raha, Abdul Rahim Suhaimi, Napis Micheal Wong, Clemente Vui Ling Gwendelyne Bulan, Tanil Mitsuaki, Nishibuchi |
| author_facet | Lesley Maurice, Bilung Son, Radu Abdul Rani, Bahaman Raha, Abdul Rahim Suhaimi, Napis Micheal Wong, Clemente Vui Ling Gwendelyne Bulan, Tanil Mitsuaki, Nishibuchi |
| author_sort | Lesley Maurice, Bilung |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | This study aimed to determine the occurrence of Vibrio parahaemolyticus in cockles (Anadara granosa) at a harvesting area and to detect the presence of virulent strains carrying the thermostable direct hemolysin (tdh) and TDH-related hemolysin genes (trh) using PCR. Of 100 samples, 62 were positive for the presence of V. parahaemolyticus with an MPN (most probable number) value greater than 3.0 (>1100 MPN per g). The PCR analysis revealed 2 samples to be positive for the tdh gene and 11 to be positive for the trh gene. Hence, these results demonstrate the presence of pathogenic V. parahaemolyticus in cockles harvested in the study area and reveal the potential risk of illness associated with their consumption |
| first_indexed | 2025-11-15T05:59:33Z |
| format | Article |
| id | unimas-2128 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T05:59:33Z |
| publishDate | 2005 |
| publisher | John Wiley & Sons Ltd |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-21282023-05-29T01:55:39Z http://ir.unimas.my/id/eprint/2128/ Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by PCR Lesley Maurice, Bilung Son, Radu Abdul Rani, Bahaman Raha, Abdul Rahim Suhaimi, Napis Micheal Wong, Clemente Vui Ling Gwendelyne Bulan, Tanil Mitsuaki, Nishibuchi AC Collections. Series. Collected works This study aimed to determine the occurrence of Vibrio parahaemolyticus in cockles (Anadara granosa) at a harvesting area and to detect the presence of virulent strains carrying the thermostable direct hemolysin (tdh) and TDH-related hemolysin genes (trh) using PCR. Of 100 samples, 62 were positive for the presence of V. parahaemolyticus with an MPN (most probable number) value greater than 3.0 (>1100 MPN per g). The PCR analysis revealed 2 samples to be positive for the tdh gene and 11 to be positive for the trh gene. Hence, these results demonstrate the presence of pathogenic V. parahaemolyticus in cockles harvested in the study area and reveal the potential risk of illness associated with their consumption John Wiley & Sons Ltd 2005 Article NonPeerReviewed text en http://ir.unimas.my/id/eprint/2128/1/Detection%20of%20Vibrio%20parahaemolyticus%20in%20cockle%20%28Anadara%20granosa%29%20by%20PCR.pdf Lesley Maurice, Bilung and Son, Radu and Abdul Rani, Bahaman and Raha, Abdul Rahim and Suhaimi, Napis and Micheal Wong, Clemente Vui Ling and Gwendelyne Bulan, Tanil and Mitsuaki, Nishibuchi (2005) Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by PCR. FEMS Microbiology Letters 252 (2005) 85-88, 252 (1). pp. 85-88. ISSN 1574-6968 http://onlinelibrary.wiley.com/ https://doi.org/10.1016/j.femsle.2005.08.053 |
| spellingShingle | AC Collections. Series. Collected works Lesley Maurice, Bilung Son, Radu Abdul Rani, Bahaman Raha, Abdul Rahim Suhaimi, Napis Micheal Wong, Clemente Vui Ling Gwendelyne Bulan, Tanil Mitsuaki, Nishibuchi Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by PCR |
| title | Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by PCR |
| title_full | Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by PCR |
| title_fullStr | Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by PCR |
| title_full_unstemmed | Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by PCR |
| title_short | Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by PCR |
| title_sort | detection of vibrio parahaemolyticus in cockle (anadara granosa) by pcr |
| topic | AC Collections. Series. Collected works |
| url | http://ir.unimas.my/id/eprint/2128/ http://ir.unimas.my/id/eprint/2128/ http://ir.unimas.my/id/eprint/2128/ http://ir.unimas.my/id/eprint/2128/1/Detection%20of%20Vibrio%20parahaemolyticus%20in%20cockle%20%28Anadara%20granosa%29%20by%20PCR.pdf |