Oxidised low density Lipoprotein decreases HIT-T15 pancreatic beta cells viability decrease in antioxidant activities
Increased serum levels of oxidised low density lipoprotein (oxLDL) as found in patients with Type 2 diabetes, can induce severe oxidative stress in exposed cells which then can lead to cell death. Our study aimed to determine how the decreased cells antioxidant status of pancreatic beta cells induce...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
malaysian society of applied biology
2017
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/19136/ http://ir.unimas.my/id/eprint/19136/1/46_04_09%20-%20%28abstrak%29.pdf |
| _version_ | 1848838648360861696 |
|---|---|
| author | Zunika, A. Muna, Sabri Rina, S. |
| author_facet | Zunika, A. Muna, Sabri Rina, S. |
| author_sort | Zunika, A. |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Increased serum levels of oxidised low density lipoprotein (oxLDL) as found in patients with Type 2 diabetes, can induce severe oxidative stress in exposed cells which then can lead to cell death. Our study aimed to determine how the decreased cells antioxidant status of pancreatic beta cells induced by oxLDL affects their viability. We used various concentrations of CuCl2 oxidised LDL to determine its cytotoxic effect as well as the influence on the antioxidant enzymes activity in HIT-T15 pancreatic beta cells. A significant cellular formation of reactive oxygen species (ROS) was detected within 3 hours incubation of HIT-T15 pancreatic beta cells with 1.5 mg/mL of oxLDL. The formation of ROS was accompanied by a simultaneously loss of cellular glutathione. However, a significant reduction in cell viability was only measured after 10 hours incubation
with oxLDL. The enzymatic activities of catalase and peroxidase remained unchanged for 24 hours. These results suggest that loss of glutathione induced by oxLDL is not sufficient to cause HIT-T15 cell death as they can be still protected from cytotoxic effects of oxLDL by the other antioxidant defenses such as catalase and peroxidase. |
| first_indexed | 2025-11-15T06:58:54Z |
| format | Article |
| id | unimas-19136 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:58:54Z |
| publishDate | 2017 |
| publisher | malaysian society of applied biology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-191362018-01-03T00:20:16Z http://ir.unimas.my/id/eprint/19136/ Oxidised low density Lipoprotein decreases HIT-T15 pancreatic beta cells viability decrease in antioxidant activities Zunika, A. Muna, Sabri Rina, S. Q Science (General) Increased serum levels of oxidised low density lipoprotein (oxLDL) as found in patients with Type 2 diabetes, can induce severe oxidative stress in exposed cells which then can lead to cell death. Our study aimed to determine how the decreased cells antioxidant status of pancreatic beta cells induced by oxLDL affects their viability. We used various concentrations of CuCl2 oxidised LDL to determine its cytotoxic effect as well as the influence on the antioxidant enzymes activity in HIT-T15 pancreatic beta cells. A significant cellular formation of reactive oxygen species (ROS) was detected within 3 hours incubation of HIT-T15 pancreatic beta cells with 1.5 mg/mL of oxLDL. The formation of ROS was accompanied by a simultaneously loss of cellular glutathione. However, a significant reduction in cell viability was only measured after 10 hours incubation with oxLDL. The enzymatic activities of catalase and peroxidase remained unchanged for 24 hours. These results suggest that loss of glutathione induced by oxLDL is not sufficient to cause HIT-T15 cell death as they can be still protected from cytotoxic effects of oxLDL by the other antioxidant defenses such as catalase and peroxidase. malaysian society of applied biology 2017-12 Article PeerReviewed text en http://ir.unimas.my/id/eprint/19136/1/46_04_09%20-%20%28abstrak%29.pdf Zunika, A. and Muna, Sabri and Rina, S. (2017) Oxidised low density Lipoprotein decreases HIT-T15 pancreatic beta cells viability decrease in antioxidant activities. Journal of the Malaysian Society of Applied Biology, 46 (4). pp. 73-83. ISSN 0126/8643 http://www.mabjournal.com |
| spellingShingle | Q Science (General) Zunika, A. Muna, Sabri Rina, S. Oxidised low density Lipoprotein decreases HIT-T15 pancreatic beta cells viability decrease in antioxidant activities |
| title | Oxidised low density Lipoprotein decreases HIT-T15 pancreatic beta cells viability decrease in antioxidant activities |
| title_full | Oxidised low density Lipoprotein decreases HIT-T15 pancreatic beta cells viability decrease in antioxidant activities |
| title_fullStr | Oxidised low density Lipoprotein decreases HIT-T15 pancreatic beta cells viability decrease in antioxidant activities |
| title_full_unstemmed | Oxidised low density Lipoprotein decreases HIT-T15 pancreatic beta cells viability decrease in antioxidant activities |
| title_short | Oxidised low density Lipoprotein decreases HIT-T15 pancreatic beta cells viability decrease in antioxidant activities |
| title_sort | oxidised low density lipoprotein decreases hit-t15 pancreatic beta cells viability decrease in antioxidant activities |
| topic | Q Science (General) |
| url | http://ir.unimas.my/id/eprint/19136/ http://ir.unimas.my/id/eprint/19136/ http://ir.unimas.my/id/eprint/19136/1/46_04_09%20-%20%28abstrak%29.pdf |