Preliminary screening of nicotianamine synthase (nas) gene in sago palm using PCR technique
Nicotianamine synthase (nas) gene is a gene involved in iron-uptake mechanisms in plants. The isolation and understanding of the function of nicotianamine synthase gene in sago palm is essential for genetic manipulation of the plant in the future. In order to accomplish this, we attempted to screen...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak (UNIMAS)
2005
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/18905/ http://ir.unimas.my/id/eprint/18905/1/Rohana%20Muhammad%20ft.pdf |
| _version_ | 1848838607225225216 |
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| author | Rohana, Binti Muhammad. |
| author_facet | Rohana, Binti Muhammad. |
| author_sort | Rohana, Binti Muhammad. |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Nicotianamine synthase (nas) gene is a gene involved in iron-uptake mechanisms in plants. The isolation and understanding of the function of nicotianamine synthase gene in sago palm is essential for genetic manipulation of the plant in the future. In order to accomplish this, we attempted to screen two sets of primers designed from rice to look for possible nas in sago. CTAB method for miniprep extraction produced satisfactory results where DNA bands were observed in 1% agarose gel electrophoresis. Once the unpurified DNA was subjected for PCR with two sets of primers, a single band approximately 650bp was observed in one set of primers. Unfortunately, the cloning step could not be proceed due to time constrain. |
| first_indexed | 2025-11-15T06:58:14Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-18905 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:58:14Z |
| publishDate | 2005 |
| publisher | Universiti Malaysia Sarawak (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-189052024-06-25T08:42:27Z http://ir.unimas.my/id/eprint/18905/ Preliminary screening of nicotianamine synthase (nas) gene in sago palm using PCR technique Rohana, Binti Muhammad. QK Botany SB Plant culture Nicotianamine synthase (nas) gene is a gene involved in iron-uptake mechanisms in plants. The isolation and understanding of the function of nicotianamine synthase gene in sago palm is essential for genetic manipulation of the plant in the future. In order to accomplish this, we attempted to screen two sets of primers designed from rice to look for possible nas in sago. CTAB method for miniprep extraction produced satisfactory results where DNA bands were observed in 1% agarose gel electrophoresis. Once the unpurified DNA was subjected for PCR with two sets of primers, a single band approximately 650bp was observed in one set of primers. Unfortunately, the cloning step could not be proceed due to time constrain. Universiti Malaysia Sarawak (UNIMAS) 2005 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/18905/1/Rohana%20Muhammad%20ft.pdf Rohana, Binti Muhammad. (2005) Preliminary screening of nicotianamine synthase (nas) gene in sago palm using PCR technique. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | QK Botany SB Plant culture Rohana, Binti Muhammad. Preliminary screening of nicotianamine synthase (nas) gene in sago palm using PCR technique |
| title | Preliminary screening of nicotianamine synthase (nas) gene in sago palm using PCR technique |
| title_full | Preliminary screening of nicotianamine synthase (nas) gene in sago palm using PCR technique |
| title_fullStr | Preliminary screening of nicotianamine synthase (nas) gene in sago palm using PCR technique |
| title_full_unstemmed | Preliminary screening of nicotianamine synthase (nas) gene in sago palm using PCR technique |
| title_short | Preliminary screening of nicotianamine synthase (nas) gene in sago palm using PCR technique |
| title_sort | preliminary screening of nicotianamine synthase (nas) gene in sago palm using pcr technique |
| topic | QK Botany SB Plant culture |
| url | http://ir.unimas.my/id/eprint/18905/ http://ir.unimas.my/id/eprint/18905/1/Rohana%20Muhammad%20ft.pdf |