Preliminary study on isolation and identification of pasteurella multocida isolated from healthy local breed rabbits
A total of 55 isolates were tentatively identified as P. mllltocida. The isolates were isolated ITom two local falms -Yu Zhou Fann in Kota Samarahan and Tan Domestic Breeder in Kuching area. Out the 55 isolates, 16 of them were tentatively identified as bacterium P. 111111tocida based on 5 isolates...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English English |
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Universiti Malaysia Sarawak (UNIMAS)
2006
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| Online Access: | http://ir.unimas.my/id/eprint/18754/ http://ir.unimas.my/id/eprint/18754/1/Kathy%20%20%2824%20pages%29.pdf http://ir.unimas.my/id/eprint/18754/4/Kathy%20%28fulltext%29.pdf |
| _version_ | 1848838575598075904 |
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| author | Sia, Kathy Shin Shin. |
| author_facet | Sia, Kathy Shin Shin. |
| author_sort | Sia, Kathy Shin Shin. |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | A total of 55 isolates were tentatively identified as P. mllltocida. The isolates were isolated ITom two local falms -Yu Zhou Fann in Kota Samarahan and Tan Domestic Breeder in Kuching area. Out the 55 isolates, 16 of them were tentatively identified as bacterium P. 111111tocida based on 5 isolates positive for oxidase production and the remanding II were negative in oxidase production. The results for conventional biochemical tests were compared with API 20E and API 20NE kits' results. However, the kits were unsatisfactory in identifying this bacterium. PFGE method were also used for genotyping the isolates. Nonetheless, the method fail to show any relatedness between the isolates due to the over excision of restriction enzyme Apal to the bacteria genome. Therefore, a few samples were selected for 16S rRNA sequencing analysis in order to identify the bacterium. The sequencing results identified that the isolates as non P. multocida. Based on the results obtained, the isolates initially identify as P. multocida were proven to be a difficult process. This indicates the difficultly in isolating this organism from healthy rabbits or this bacterium is actually uncommon in the local breed rabbits. |
| first_indexed | 2025-11-15T06:57:44Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-18754 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T06:57:44Z |
| publishDate | 2006 |
| publisher | Universiti Malaysia Sarawak (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-187542024-03-06T07:21:23Z http://ir.unimas.my/id/eprint/18754/ Preliminary study on isolation and identification of pasteurella multocida isolated from healthy local breed rabbits Sia, Kathy Shin Shin. QD Chemistry QR Microbiology A total of 55 isolates were tentatively identified as P. mllltocida. The isolates were isolated ITom two local falms -Yu Zhou Fann in Kota Samarahan and Tan Domestic Breeder in Kuching area. Out the 55 isolates, 16 of them were tentatively identified as bacterium P. 111111tocida based on 5 isolates positive for oxidase production and the remanding II were negative in oxidase production. The results for conventional biochemical tests were compared with API 20E and API 20NE kits' results. However, the kits were unsatisfactory in identifying this bacterium. PFGE method were also used for genotyping the isolates. Nonetheless, the method fail to show any relatedness between the isolates due to the over excision of restriction enzyme Apal to the bacteria genome. Therefore, a few samples were selected for 16S rRNA sequencing analysis in order to identify the bacterium. The sequencing results identified that the isolates as non P. multocida. Based on the results obtained, the isolates initially identify as P. multocida were proven to be a difficult process. This indicates the difficultly in isolating this organism from healthy rabbits or this bacterium is actually uncommon in the local breed rabbits. Universiti Malaysia Sarawak (UNIMAS) 2006 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/18754/1/Kathy%20%20%2824%20pages%29.pdf text en http://ir.unimas.my/id/eprint/18754/4/Kathy%20%28fulltext%29.pdf Sia, Kathy Shin Shin. (2006) Preliminary study on isolation and identification of pasteurella multocida isolated from healthy local breed rabbits. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | QD Chemistry QR Microbiology Sia, Kathy Shin Shin. Preliminary study on isolation and identification of pasteurella multocida isolated from healthy local breed rabbits |
| title | Preliminary study on isolation and identification of pasteurella multocida isolated from healthy local breed rabbits |
| title_full | Preliminary study on isolation and identification of pasteurella multocida isolated from healthy local breed rabbits |
| title_fullStr | Preliminary study on isolation and identification of pasteurella multocida isolated from healthy local breed rabbits |
| title_full_unstemmed | Preliminary study on isolation and identification of pasteurella multocida isolated from healthy local breed rabbits |
| title_short | Preliminary study on isolation and identification of pasteurella multocida isolated from healthy local breed rabbits |
| title_sort | preliminary study on isolation and identification of pasteurella multocida isolated from healthy local breed rabbits |
| topic | QD Chemistry QR Microbiology |
| url | http://ir.unimas.my/id/eprint/18754/ http://ir.unimas.my/id/eprint/18754/1/Kathy%20%20%2824%20pages%29.pdf http://ir.unimas.my/id/eprint/18754/4/Kathy%20%28fulltext%29.pdf |