Ancient Divergence Time Estimates In Eutropis Rugifera Support The Existence Of Pleistocene Barriers On The Exposed Sunda Shelf

Episodic sea level changes that repeatedly exposed and inundated the Sunda Shelf characterize the Pleistocene. Available evidence points to a more xeric central Sunda Shelf during periods of low sea levels, and despite the broad land connections that persisted during this time, some organisms are...

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Main Authors: Karin, B.R, Das, I., Jackman, Todd R., Bauer, Aaron M.
Format: Article
Language:English
Published: PeerJ 2017
Subjects:
Online Access:http://ir.unimas.my/id/eprint/18584/
http://ir.unimas.my/id/eprint/18584/7/Ancient%20divergence%20time%20estimates%20%28abstract%29.pdf
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author Karin, B.R
Das, I.
Jackman, Todd R.
Bauer, Aaron M.
author_facet Karin, B.R
Das, I.
Jackman, Todd R.
Bauer, Aaron M.
author_sort Karin, B.R
building UNIMAS Institutional Repository
collection Online Access
description Episodic sea level changes that repeatedly exposed and inundated the Sunda Shelf characterize the Pleistocene. Available evidence points to a more xeric central Sunda Shelf during periods of low sea levels, and despite the broad land connections that persisted during this time, some organisms are assumed to have faced barriers to dispersal between land-masses on the Sunda Shelf. Eutropis rugifera is a secretive, forest adapted scincid lizard that ranges across the Sunda Shelf. In this study, we sequenced one mitochondrial (ND2) and four nuclear (BRCA1, BRCA2, RAG1, and MC1R) markers and generated a time-calibrated phylogeny in BEAST to test whether divergence times between Sundaic populations of E. rugifera occurred during Pleistocene sea-level changes, or if they predate the Pleistocene. We find that E. rugifera shows pre-Pleistocene divergences between populations on different Sundaic land-masses. The earliest divergence within E. rugifera separates the Philippine samples from the Sundaic samples approximately 16 Ma; the Philippine populations thus cannot be considered conspecific with Sundaic congeners. Sundaic populations diverged approximately 6 Ma, and populations within Borneo from Sabah and Sarawak separated approximately 4.5 Ma in the early Pliocene, followed by further cladogenesis in Sarawak through the Pleistocene. Divergence of peninsular Malaysian populations from the Mentawai Archipelago occurred approximately 5 Ma. Separation among island populations from the Mentawai Archipelago likely dates to the Pliocene/Pleistocene boundary approximately 3.5 Ma, and our samples from peninsular Malaysia appear to coalesce in the middle Pleistocene, about 1 Ma. Coupled with the monophyly of these populations, these divergence times suggest that despite consistent land-connections between these regions throughout the Pleistocene E. rugifera still faced barriers to dispersal, which may be a result of environmental shifts that accompanied the sea-level changes.
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spelling unimas-185842023-03-31T07:44:27Z http://ir.unimas.my/id/eprint/18584/ Ancient Divergence Time Estimates In Eutropis Rugifera Support The Existence Of Pleistocene Barriers On The Exposed Sunda Shelf Karin, B.R Das, I. Jackman, Todd R. Bauer, Aaron M. Q Science (General) Episodic sea level changes that repeatedly exposed and inundated the Sunda Shelf characterize the Pleistocene. Available evidence points to a more xeric central Sunda Shelf during periods of low sea levels, and despite the broad land connections that persisted during this time, some organisms are assumed to have faced barriers to dispersal between land-masses on the Sunda Shelf. Eutropis rugifera is a secretive, forest adapted scincid lizard that ranges across the Sunda Shelf. In this study, we sequenced one mitochondrial (ND2) and four nuclear (BRCA1, BRCA2, RAG1, and MC1R) markers and generated a time-calibrated phylogeny in BEAST to test whether divergence times between Sundaic populations of E. rugifera occurred during Pleistocene sea-level changes, or if they predate the Pleistocene. We find that E. rugifera shows pre-Pleistocene divergences between populations on different Sundaic land-masses. The earliest divergence within E. rugifera separates the Philippine samples from the Sundaic samples approximately 16 Ma; the Philippine populations thus cannot be considered conspecific with Sundaic congeners. Sundaic populations diverged approximately 6 Ma, and populations within Borneo from Sabah and Sarawak separated approximately 4.5 Ma in the early Pliocene, followed by further cladogenesis in Sarawak through the Pleistocene. Divergence of peninsular Malaysian populations from the Mentawai Archipelago occurred approximately 5 Ma. Separation among island populations from the Mentawai Archipelago likely dates to the Pliocene/Pleistocene boundary approximately 3.5 Ma, and our samples from peninsular Malaysia appear to coalesce in the middle Pleistocene, about 1 Ma. Coupled with the monophyly of these populations, these divergence times suggest that despite consistent land-connections between these regions throughout the Pleistocene E. rugifera still faced barriers to dispersal, which may be a result of environmental shifts that accompanied the sea-level changes. PeerJ 2017-10-27 Article PeerReviewed text en http://ir.unimas.my/id/eprint/18584/7/Ancient%20divergence%20time%20estimates%20%28abstract%29.pdf Karin, B.R and Das, I. and Jackman, Todd R. and Bauer, Aaron M. (2017) Ancient Divergence Time Estimates In Eutropis Rugifera Support The Existence Of Pleistocene Barriers On The Exposed Sunda Shelf. Peer J, 5. ISSN 2376-5992 https://peerj.com/articles/3762/ DOI 10.7717/peerj.3762
spellingShingle Q Science (General)
Karin, B.R
Das, I.
Jackman, Todd R.
Bauer, Aaron M.
Ancient Divergence Time Estimates In Eutropis Rugifera Support The Existence Of Pleistocene Barriers On The Exposed Sunda Shelf
title Ancient Divergence Time Estimates In Eutropis Rugifera Support The Existence Of Pleistocene Barriers On The Exposed Sunda Shelf
title_full Ancient Divergence Time Estimates In Eutropis Rugifera Support The Existence Of Pleistocene Barriers On The Exposed Sunda Shelf
title_fullStr Ancient Divergence Time Estimates In Eutropis Rugifera Support The Existence Of Pleistocene Barriers On The Exposed Sunda Shelf
title_full_unstemmed Ancient Divergence Time Estimates In Eutropis Rugifera Support The Existence Of Pleistocene Barriers On The Exposed Sunda Shelf
title_short Ancient Divergence Time Estimates In Eutropis Rugifera Support The Existence Of Pleistocene Barriers On The Exposed Sunda Shelf
title_sort ancient divergence time estimates in eutropis rugifera support the existence of pleistocene barriers on the exposed sunda shelf
topic Q Science (General)
url http://ir.unimas.my/id/eprint/18584/
http://ir.unimas.my/id/eprint/18584/
http://ir.unimas.my/id/eprint/18584/
http://ir.unimas.my/id/eprint/18584/7/Ancient%20divergence%20time%20estimates%20%28abstract%29.pdf