Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production
Background: The increasing market demand for oligosaccharides has intensified the need for efficient biocatalysts. Glycosyl hydrolases (GHs) are still gaining popularity as biocatalyst for oligosaccharides synthesis owing to its simple reaction and high selectivity. Purpose: Over the years, research...
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| Format: | Article |
| Language: | English |
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Taylor and Francis Ltd
2017
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| Online Access: | http://ir.unimas.my/id/eprint/18182/ http://ir.unimas.my/id/eprint/18182/2/Strategy%20in.pdf |
| _version_ | 1848838456198823936 |
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| author | Nor Hasmaliana, Abdul Manas Rosli, Md. Illias Nor Muhammad, Mahadi |
| author_facet | Nor Hasmaliana, Abdul Manas Rosli, Md. Illias Nor Muhammad, Mahadi |
| author_sort | Nor Hasmaliana, Abdul Manas |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Background: The increasing market demand for oligosaccharides has intensified the need for efficient biocatalysts. Glycosyl hydrolases (GHs) are still gaining popularity as biocatalyst for oligosaccharides synthesis owing to its simple reaction and high selectivity. Purpose: Over the years, research has advanced mainly directing to one goal; to reduce hydrolysis activity of GHs for increased transglycosylation activity in achieving high production of oligosaccharides. Design and methods: This review concisely presents the strategies to increase transglycosylation activity of GHs for oligosaccharides synthesis, focusing on controlling the reaction equilibrium, and protein engineering. Various modifications of the subsites of GHs have been demonstrated to significantly modulate the hydrolysis and transglycosylation activity of the enzymes. The clear insight of the roles of each amino acid in these sites provides a platform for designing an enzyme that could synthesize a specific oligosaccharide product. Conclusions: The key strategies presented here are important for future improvement of GHs as a biocatalyst for oligosaccharide synthesis. © 2017 Informa UK Limited, trading as Taylor & Francis Group |
| first_indexed | 2025-11-15T06:55:50Z |
| format | Article |
| id | unimas-18182 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:55:50Z |
| publishDate | 2017 |
| publisher | Taylor and Francis Ltd |
| recordtype | eprints |
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| spelling | unimas-181822022-06-22T02:07:44Z http://ir.unimas.my/id/eprint/18182/ Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production Nor Hasmaliana, Abdul Manas Rosli, Md. Illias Nor Muhammad, Mahadi QR180 Immunology Background: The increasing market demand for oligosaccharides has intensified the need for efficient biocatalysts. Glycosyl hydrolases (GHs) are still gaining popularity as biocatalyst for oligosaccharides synthesis owing to its simple reaction and high selectivity. Purpose: Over the years, research has advanced mainly directing to one goal; to reduce hydrolysis activity of GHs for increased transglycosylation activity in achieving high production of oligosaccharides. Design and methods: This review concisely presents the strategies to increase transglycosylation activity of GHs for oligosaccharides synthesis, focusing on controlling the reaction equilibrium, and protein engineering. Various modifications of the subsites of GHs have been demonstrated to significantly modulate the hydrolysis and transglycosylation activity of the enzymes. The clear insight of the roles of each amino acid in these sites provides a platform for designing an enzyme that could synthesize a specific oligosaccharide product. Conclusions: The key strategies presented here are important for future improvement of GHs as a biocatalyst for oligosaccharide synthesis. © 2017 Informa UK Limited, trading as Taylor & Francis Group Taylor and Francis Ltd 2017 Article PeerReviewed text en http://ir.unimas.my/id/eprint/18182/2/Strategy%20in.pdf Nor Hasmaliana, Abdul Manas and Rosli, Md. Illias and Nor Muhammad, Mahadi (2017) Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production. Critical Reviews in Biotechnology, 38 (2). pp. 1-22. ISSN 0738-8551 https://www.tandfonline.com/doi/abs/10.1080/07388551.2017.1339664?journalCode=ibty20 DOI: 10.1080/07388551.2017.1339664 |
| spellingShingle | QR180 Immunology Nor Hasmaliana, Abdul Manas Rosli, Md. Illias Nor Muhammad, Mahadi Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production |
| title | Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production |
| title_full | Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production |
| title_fullStr | Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production |
| title_full_unstemmed | Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production |
| title_short | Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production |
| title_sort | strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production |
| topic | QR180 Immunology |
| url | http://ir.unimas.my/id/eprint/18182/ http://ir.unimas.my/id/eprint/18182/ http://ir.unimas.my/id/eprint/18182/ http://ir.unimas.my/id/eprint/18182/2/Strategy%20in.pdf |