Preliminary work on molecular identification of markers linked to papaya sex gene locus
Carica papaya is a polygamous plant species with male, female and hermaphrodite sexes. Genetic studies have shown that sex in papaya is controlled by a major gene SexI with three alleles. However, the sex of the plant can not be identified through morphological characteristics. Since only the fruits...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak (UNIMAS)
2006
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| Online Access: | http://ir.unimas.my/id/eprint/18080/ http://ir.unimas.my/id/eprint/18080/3/Jenny%20Lee.pdf |
| _version_ | 1848838431067602944 |
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| author | Lee, Jenny |
| author_facet | Lee, Jenny |
| author_sort | Lee, Jenny |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Carica papaya is a polygamous plant species with male, female and hermaphrodite sexes. Genetic studies have shown that sex in papaya is controlled by a major gene SexI with three alleles. However, the sex of the plant can not be identified through morphological characteristics. Since only the fruits from hermaphrodite plants are in greater demand and the female plants can only be rouged out after reaching the flowering stage, development of a molecular marker would be usefu'l to papaya growers. The objective of this project is to identify any diagnostic bands that are linked to papaya sex determination gene. In this study, both female and hermaphrodite leaf samples of Sekaki cultivar were collected for analysis. The genomic DNA was successfully isolated from papaya leaves using the modified CT AB method. DAMD-PCR technique was used to detect any sex-specific patterns. Limited polymorphic banding profiles for female and hermaphrodite samples were generated using MI3 universal primer in this study. However, no diagnostic band was detected. |
| first_indexed | 2025-11-15T06:55:26Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-18080 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:55:26Z |
| publishDate | 2006 |
| publisher | Universiti Malaysia Sarawak (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-180802024-01-03T07:45:43Z http://ir.unimas.my/id/eprint/18080/ Preliminary work on molecular identification of markers linked to papaya sex gene locus Lee, Jenny QK Botany SB Plant culture Carica papaya is a polygamous plant species with male, female and hermaphrodite sexes. Genetic studies have shown that sex in papaya is controlled by a major gene SexI with three alleles. However, the sex of the plant can not be identified through morphological characteristics. Since only the fruits from hermaphrodite plants are in greater demand and the female plants can only be rouged out after reaching the flowering stage, development of a molecular marker would be usefu'l to papaya growers. The objective of this project is to identify any diagnostic bands that are linked to papaya sex determination gene. In this study, both female and hermaphrodite leaf samples of Sekaki cultivar were collected for analysis. The genomic DNA was successfully isolated from papaya leaves using the modified CT AB method. DAMD-PCR technique was used to detect any sex-specific patterns. Limited polymorphic banding profiles for female and hermaphrodite samples were generated using MI3 universal primer in this study. However, no diagnostic band was detected. Universiti Malaysia Sarawak (UNIMAS) 2006 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/18080/3/Jenny%20Lee.pdf Lee, Jenny (2006) Preliminary work on molecular identification of markers linked to papaya sex gene locus. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | QK Botany SB Plant culture Lee, Jenny Preliminary work on molecular identification of markers linked to papaya sex gene locus |
| title | Preliminary work on molecular identification of markers linked to papaya sex gene locus |
| title_full | Preliminary work on molecular identification of markers linked to papaya sex gene locus |
| title_fullStr | Preliminary work on molecular identification of markers linked to papaya sex gene locus |
| title_full_unstemmed | Preliminary work on molecular identification of markers linked to papaya sex gene locus |
| title_short | Preliminary work on molecular identification of markers linked to papaya sex gene locus |
| title_sort | preliminary work on molecular identification of markers linked to papaya sex gene locus |
| topic | QK Botany SB Plant culture |
| url | http://ir.unimas.my/id/eprint/18080/ http://ir.unimas.my/id/eprint/18080/3/Jenny%20Lee.pdf |