Restriction site mapping analysis of the adenomatous poliposis coli (APC) gene in human colorectal system
Adenomatous Poliposis Coli (APC) anomalies are considered to playa gate-keeping role in colorectal tumorigenesis. Even though APC gene is extensively studied, there is a lack in effort towards creating a highly reproducible and less costly approach to diagnose APC anomalies. In this research, the AP...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak (UNIMAS)
2005
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| Online Access: | http://ir.unimas.my/id/eprint/18074/ http://ir.unimas.my/id/eprint/18074/8/Restriction%20site%20mapping%20analysis%20of%20the%20Adenomatous%20poliposis%20coli%20%28APC%29%20gene%20in%20human%20colorectal%20system%20%28fulltext%29.pdf |
| _version_ | 1848838429377298432 |
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| author | Chung, James Yaw Ming. |
| author_facet | Chung, James Yaw Ming. |
| author_sort | Chung, James Yaw Ming. |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Adenomatous Poliposis Coli (APC) anomalies are considered to playa gate-keeping role in colorectal tumorigenesis. Even though APC gene is extensively studied, there is a lack in effort towards creating a highly reproducible and less costly approach to diagnose APC anomalies. In this research, the APC cDNA fragments from the normal and tumour colorectal specimens are subjected to mutational analysis via restriction enzyme site characterization. Restriction digestions using AluI, HaeIII, Hinfl, HincH, HpaII, MboI, NdeI, RsaI, TaqI, and Trn9I were performed on all eight APC fragments of normal and tumour specimens accordingly. Restriction site map for the gene was constructed whereby assay on APC 2, APC 5, and APC 6 had generated differential restriction site pattern. Tumour APC 2 was showing aberrancy on RsaI, Hinfl, Trn9I, HaeIII, and HpaH; whereas tumour APC 5 was aberrant on AluI, and tumour APC 6 was aberrant on TaqI. This will facilitate the recognition of potential mutation marker to screen for APC aberrancy, allowing the comparative study between APC restriction site pattern of normal and tumour system. |
| first_indexed | 2025-11-15T06:55:25Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-18074 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:55:25Z |
| publishDate | 2005 |
| publisher | Universiti Malaysia Sarawak (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-180742023-02-13T08:28:39Z http://ir.unimas.my/id/eprint/18074/ Restriction site mapping analysis of the adenomatous poliposis coli (APC) gene in human colorectal system Chung, James Yaw Ming. QR Microbiology Adenomatous Poliposis Coli (APC) anomalies are considered to playa gate-keeping role in colorectal tumorigenesis. Even though APC gene is extensively studied, there is a lack in effort towards creating a highly reproducible and less costly approach to diagnose APC anomalies. In this research, the APC cDNA fragments from the normal and tumour colorectal specimens are subjected to mutational analysis via restriction enzyme site characterization. Restriction digestions using AluI, HaeIII, Hinfl, HincH, HpaII, MboI, NdeI, RsaI, TaqI, and Trn9I were performed on all eight APC fragments of normal and tumour specimens accordingly. Restriction site map for the gene was constructed whereby assay on APC 2, APC 5, and APC 6 had generated differential restriction site pattern. Tumour APC 2 was showing aberrancy on RsaI, Hinfl, Trn9I, HaeIII, and HpaH; whereas tumour APC 5 was aberrant on AluI, and tumour APC 6 was aberrant on TaqI. This will facilitate the recognition of potential mutation marker to screen for APC aberrancy, allowing the comparative study between APC restriction site pattern of normal and tumour system. Universiti Malaysia Sarawak (UNIMAS) 2005 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/18074/8/Restriction%20site%20mapping%20analysis%20of%20the%20Adenomatous%20poliposis%20coli%20%28APC%29%20gene%20in%20human%20colorectal%20system%20%28fulltext%29.pdf Chung, James Yaw Ming. (2005) Restriction site mapping analysis of the adenomatous poliposis coli (APC) gene in human colorectal system. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | QR Microbiology Chung, James Yaw Ming. Restriction site mapping analysis of the adenomatous poliposis coli (APC) gene in human colorectal system |
| title | Restriction site mapping analysis of the adenomatous poliposis coli (APC) gene in human colorectal system |
| title_full | Restriction site mapping analysis of the adenomatous poliposis coli (APC) gene in human colorectal system |
| title_fullStr | Restriction site mapping analysis of the adenomatous poliposis coli (APC) gene in human colorectal system |
| title_full_unstemmed | Restriction site mapping analysis of the adenomatous poliposis coli (APC) gene in human colorectal system |
| title_short | Restriction site mapping analysis of the adenomatous poliposis coli (APC) gene in human colorectal system |
| title_sort | restriction site mapping analysis of the adenomatous poliposis coli (apc) gene in human colorectal system |
| topic | QR Microbiology |
| url | http://ir.unimas.my/id/eprint/18074/ http://ir.unimas.my/id/eprint/18074/8/Restriction%20site%20mapping%20analysis%20of%20the%20Adenomatous%20poliposis%20coli%20%28APC%29%20gene%20in%20human%20colorectal%20system%20%28fulltext%29.pdf |