Screening, isolation and molecular characterization of @-amylase enzyme from basillus amyloliquefaciens umas 1002
This study concerned about the screening, isolating and molecular characteristic of a-amylase gene of Bacillus amyloliquefaciens UMAS 1002. This cellulolytic and amylolytic bacterium was isolated from sago pith waste grew in minimal starch and carboxymethy1cellulose media. The production of a-amylas...
| Main Author: | |
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak (UNIMAS)
2003
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/17930/ http://ir.unimas.my/id/eprint/17930/2/Husna%20Mohd%20Noor.pdf |
| _version_ | 1848838399048286208 |
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| author | Husna, Hj. Mohd Noor. |
| author_facet | Husna, Hj. Mohd Noor. |
| author_sort | Husna, Hj. Mohd Noor. |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | This study concerned about the screening, isolating and molecular characteristic of a-amylase gene of Bacillus amyloliquefaciens UMAS 1002. This cellulolytic and amylolytic bacterium was isolated from sago pith waste grew in minimal starch and carboxymethy1cellulose media. The production of a-amylase enzyme was undertaken by microbial degradation of the media that produced clear haloes around the colonies indicated amylolytic and cellulolytic activity. These colonies were selected for molecular analysis;
genomic DNA extraction, restriction endonuclease digestion, Polymerase Chain Reaction (PCR), and genomic cloning. Since there are some problems, a gene encoding extracellular
a-amylase enzyme could not be cloned and sequenced. |
| first_indexed | 2025-11-15T06:54:56Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-17930 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:54:56Z |
| publishDate | 2003 |
| publisher | Universiti Malaysia Sarawak (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-179302023-11-27T09:04:51Z http://ir.unimas.my/id/eprint/17930/ Screening, isolation and molecular characterization of @-amylase enzyme from basillus amyloliquefaciens umas 1002 Husna, Hj. Mohd Noor. QR Microbiology This study concerned about the screening, isolating and molecular characteristic of a-amylase gene of Bacillus amyloliquefaciens UMAS 1002. This cellulolytic and amylolytic bacterium was isolated from sago pith waste grew in minimal starch and carboxymethy1cellulose media. The production of a-amylase enzyme was undertaken by microbial degradation of the media that produced clear haloes around the colonies indicated amylolytic and cellulolytic activity. These colonies were selected for molecular analysis; genomic DNA extraction, restriction endonuclease digestion, Polymerase Chain Reaction (PCR), and genomic cloning. Since there are some problems, a gene encoding extracellular a-amylase enzyme could not be cloned and sequenced. Universiti Malaysia Sarawak (UNIMAS) 2003 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/17930/2/Husna%20Mohd%20Noor.pdf Husna, Hj. Mohd Noor. (2003) Screening, isolation and molecular characterization of @-amylase enzyme from basillus amyloliquefaciens umas 1002. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | QR Microbiology Husna, Hj. Mohd Noor. Screening, isolation and molecular characterization of @-amylase enzyme from basillus amyloliquefaciens umas 1002 |
| title | Screening, isolation and molecular characterization of @-amylase enzyme from basillus amyloliquefaciens umas 1002 |
| title_full | Screening, isolation and molecular characterization of @-amylase enzyme from basillus amyloliquefaciens umas 1002 |
| title_fullStr | Screening, isolation and molecular characterization of @-amylase enzyme from basillus amyloliquefaciens umas 1002 |
| title_full_unstemmed | Screening, isolation and molecular characterization of @-amylase enzyme from basillus amyloliquefaciens umas 1002 |
| title_short | Screening, isolation and molecular characterization of @-amylase enzyme from basillus amyloliquefaciens umas 1002 |
| title_sort | screening, isolation and molecular characterization of @-amylase enzyme from basillus amyloliquefaciens umas 1002 |
| topic | QR Microbiology |
| url | http://ir.unimas.my/id/eprint/17930/ http://ir.unimas.my/id/eprint/17930/2/Husna%20Mohd%20Noor.pdf |