Screening, isolation and molecular characterization of @-amylase enzyme from basillus amyloliquefaciens umas 1002
This study concerned about the screening, isolating and molecular characteristic of a-amylase gene of Bacillus amyloliquefaciens UMAS 1002. This cellulolytic and amylolytic bacterium was isolated from sago pith waste grew in minimal starch and carboxymethy1cellulose media. The production of a-amylas...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak (UNIMAS)
2003
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| Online Access: | http://ir.unimas.my/id/eprint/17930/ http://ir.unimas.my/id/eprint/17930/2/Husna%20Mohd%20Noor.pdf |
| Summary: | This study concerned about the screening, isolating and molecular characteristic of a-amylase gene of Bacillus amyloliquefaciens UMAS 1002. This cellulolytic and amylolytic bacterium was isolated from sago pith waste grew in minimal starch and carboxymethy1cellulose media. The production of a-amylase enzyme was undertaken by microbial degradation of the media that produced clear haloes around the colonies indicated amylolytic and cellulolytic activity. These colonies were selected for molecular analysis;
genomic DNA extraction, restriction endonuclease digestion, Polymerase Chain Reaction (PCR), and genomic cloning. Since there are some problems, a gene encoding extracellular
a-amylase enzyme could not be cloned and sequenced. |
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