Population genetic structure of Hampala macrolepidota (Cyprinidae) in several populations in Malaysia using random amplified polymorphic DNA (RAPD) technique

This study examined thirty individuals of Hampala macrolepidota (Bleeker) using Random amplified polymorphic DNA (RAPD) -peR technique. Samples were collected from ten different locations in Malaysia. The RAPD study identified five primers useful for population analysis. 37.50% of the RAPD loci were...

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Main Author: Die, Ling Ling
Format: Final Year Project Report / IMRAD
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2005
Subjects:
Online Access:http://ir.unimas.my/id/eprint/17740/
http://ir.unimas.my/id/eprint/17740/1/Dia%20Ling%20Ling%20ft.pdf
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author Die, Ling Ling
author_facet Die, Ling Ling
author_sort Die, Ling Ling
building UNIMAS Institutional Repository
collection Online Access
description This study examined thirty individuals of Hampala macrolepidota (Bleeker) using Random amplified polymorphic DNA (RAPD) -peR technique. Samples were collected from ten different locations in Malaysia. The RAPD study identified five primers useful for population analysis. 37.50% of the RAPD loci were polymorhic. Mean Nei's (1973) gene diversity was 0.1016. While, mean GST value across loci was 0.735, and the average for Nm values was 0.1798. The population's genetic distance was similar with the observed individual's pair-wise distance. The close relationship between H. macrolepidota samples was indicated by their low pair-wise genetic distance. In addition, the high percentage of sharing of banding profiles obtained also suggested that the If. macrolepidota samples from Peninsular Malaysia were closely related to the samples from Sarawak.
first_indexed 2025-11-15T06:54:16Z
format Final Year Project Report / IMRAD
id unimas-17740
institution Universiti Malaysia Sarawak
institution_category Local University
language English
last_indexed 2025-11-15T06:54:16Z
publishDate 2005
publisher Universiti Malaysia Sarawak, (UNIMAS)
recordtype eprints
repository_type Digital Repository
spelling unimas-177402023-07-11T04:58:05Z http://ir.unimas.my/id/eprint/17740/ Population genetic structure of Hampala macrolepidota (Cyprinidae) in several populations in Malaysia using random amplified polymorphic DNA (RAPD) technique Die, Ling Ling QL Zoology SH Aquaculture. Fisheries. Angling This study examined thirty individuals of Hampala macrolepidota (Bleeker) using Random amplified polymorphic DNA (RAPD) -peR technique. Samples were collected from ten different locations in Malaysia. The RAPD study identified five primers useful for population analysis. 37.50% of the RAPD loci were polymorhic. Mean Nei's (1973) gene diversity was 0.1016. While, mean GST value across loci was 0.735, and the average for Nm values was 0.1798. The population's genetic distance was similar with the observed individual's pair-wise distance. The close relationship between H. macrolepidota samples was indicated by their low pair-wise genetic distance. In addition, the high percentage of sharing of banding profiles obtained also suggested that the If. macrolepidota samples from Peninsular Malaysia were closely related to the samples from Sarawak. Universiti Malaysia Sarawak, (UNIMAS) 2005 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/17740/1/Dia%20Ling%20Ling%20ft.pdf Die, Ling Ling (2005) Population genetic structure of Hampala macrolepidota (Cyprinidae) in several populations in Malaysia using random amplified polymorphic DNA (RAPD) technique. [Final Year Project Report / IMRAD] (Unpublished)
spellingShingle QL Zoology
SH Aquaculture. Fisheries. Angling
Die, Ling Ling
Population genetic structure of Hampala macrolepidota (Cyprinidae) in several populations in Malaysia using random amplified polymorphic DNA (RAPD) technique
title Population genetic structure of Hampala macrolepidota (Cyprinidae) in several populations in Malaysia using random amplified polymorphic DNA (RAPD) technique
title_full Population genetic structure of Hampala macrolepidota (Cyprinidae) in several populations in Malaysia using random amplified polymorphic DNA (RAPD) technique
title_fullStr Population genetic structure of Hampala macrolepidota (Cyprinidae) in several populations in Malaysia using random amplified polymorphic DNA (RAPD) technique
title_full_unstemmed Population genetic structure of Hampala macrolepidota (Cyprinidae) in several populations in Malaysia using random amplified polymorphic DNA (RAPD) technique
title_short Population genetic structure of Hampala macrolepidota (Cyprinidae) in several populations in Malaysia using random amplified polymorphic DNA (RAPD) technique
title_sort population genetic structure of hampala macrolepidota (cyprinidae) in several populations in malaysia using random amplified polymorphic dna (rapd) technique
topic QL Zoology
SH Aquaculture. Fisheries. Angling
url http://ir.unimas.my/id/eprint/17740/
http://ir.unimas.my/id/eprint/17740/1/Dia%20Ling%20Ling%20ft.pdf